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An FTIR study of Pseudomonas aeruginosa PAO1 biofilm growth and dispersion. An improved ATR method for studying biofilms: the C–H stretch spectral region

Published online by Cambridge University Press:  24 January 2005

J. Pink ,
T. Smith-Palmer ,
T. J. Beveridge  and
D. A. Pink
J. Pink
Affiliation:
Department of Human Nutrition, St Francis Xavier University, Antigonish, NS, B2G 2W5, Canada
T. Smith-Palmer
Affiliation:
Department of Chemistry, St Francis Xavier University, Antigonish, NS, B2G 2W5, Canada
T. J. Beveridge
Affiliation:
Department of Microbiology and Advanced Foods & Materials Network – National Centre of Excellence (AFMnet-NCE), College of Biological Science, University of Guelph, Guelph, ON, N1G 2W1, Canada
D. A. Pink
Affiliation:
Department of Physics and Advanced Foods & Materials Network – National Centre of Excellence (AFMnet-NCE), St Francis Xavier University, Antigonish, NS, B2G 2W5 Canada
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Abstract

Pseudomonas aeruginosa PAO1 was grown on 40° ZnSe and 40° AMTIR® (Amorphous Material Transmitting Infrared Radiation, Ge33As12Se55) internal reflection elements in a thermostatted flow cell. Information about the chemistry of the biofilm development was obtained using Fourier transform infrared spectroscopy. The weakly absorbing C–H stretching region of the infrared spectrum was measured for the first time and revealed qualitative and quantitative changes not evident in the amide region studied by previous investigators. There is a chemical difference between the early growth period and subsequent development of the biofilm.

Type
Research Articles
Information
Biofilms , Volume 1 , Issue 3 , July 2004 , pp. 157 - 163
Copyright
© 2004 Cambridge University Press

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