This paper presents the simulator SHADYS (‘simulator halieutique de dynamiques spatiales’). SHADYS is a tool devoted to the representation of spatial processes involved in fisheries dynamics. It puts together three fundamental entities in an explicit spatio-temporal way: the environment, the fish populations under consideration and the fishing fleet. It is based on simple, realistic and well identified mechanisms (density-dependent habitat selection, advection and diffusion of a fish population, fishermen search strategy, etc.) implemented by interfacing numerical models with a GIS (geographic information system). As a virtual laboratory, SHADYS allows experiments to be performed in a theoretical world, such experiments being generally impossible to perform in reality. As an illustration, different scenarios of a marine refugia-based management have been explored using the SHADYS simulator. Different simulations are conducted to assess the short-term effects on yield of a marine protected area. It is shown that for diffusive or migratory species, the yield per recruit as a function of the protected surface can reach a maximum. Under this condition, the concept of ‘space overfishing’ is meaningful. Protected areas then behave like ‘sources’ and exploited areas like ‘sinks’. For resident populations, the larger the protected area is, the lower the catches per recruit are. Regarding the spatial distribution of the fishing effort, it is shown that if it is spatially distributed in order to maximize catches, then fishing boats will slowly tend to be distributed all along the boundaries of the protected area.

The behaviour and spatial distribution of tuna, aggregated beneath fish aggregating devices (FADs), have been studied through ultrasonic tagging experiments but, surprisingly, very few studies on FADs have used underwater acoustic devices. We present techniques, and their limits, incorporating a scientific echo sounder connected to a split-beam transducer to observe and characterise tuna aggregations around FADs, and propose a general approach for future studies. Experiments were conducted in French Polynesia between December 1995 and February 1997. Two methods, echo-counting and echo integration, were used. Echo-counting is possible when individual fish are sufficiently scattered so that each target can be discerned. On the other hand, echo integration can be used with both scattered and aggregated fish schools. The knowledge of tuna target strength is useful for separating targets for echo-counting, and essential for obtaining absolute estimates of densities by echo integration. Sonar performances and settings should be considered when choosing the most suitable method to determine fish density or assessing spatial structure of a tuna aggregation. These techniques allow one to study an entire tuna aggregation, its behaviour in space and time at very fine time–space scales (about a nautical mile and over a few hours), and open up a new scientific field to study the spatial structure and behaviour of tuna aggregations around anchored or drifting FADs.

An assessment of the state of the fisheries of hake, red mullet and Norway lobster was carried out by using trawl survey data from the MEDITS research programme. The assessment was performed by means of the application of a composite model utilising the total mortality rate Z as a direct index of fishing effort. The studied area covered the whole western Italian coast (Ligurian and Tyrrhenian seas) and the eastern coasts of Corsica. The area was divided into twelve sub-areas, considered to be of similar ecological characteristics with similar initial productivity but considered to experience different rates of exploitation. Available information on fishing fleets and their geographic distribution was used in creating sub-areas. For each one of these sub-areas, the total mortality rates and a mean catch per unit of effort were estimated. The estimation of Z was performed by analysing the size structure of the cumulated catch in each sub-area and for each year. Catch rates were estimated as the mean catch per one hour of tow. The positioning of the current values of Z for the different sub-areas relative to the Z at maximum biological production suggests that, in most of the sub-areas, hake is in general fully exploited and red mullet overexploited. In the case of Norway lobster, it was not possible to obtain a statistically acceptable result probably due to a lack of contrasting enough levels of fishing pressure among sub-areas. The results obtained for hake and red mullet are in good agreement with the available information on the consistency of the fleets operating in the different sub-areas.

Great variations were observed in length-at-age among populations of white sucker, Catostomus commersoni, in 32 Ontario lakes. The spatial structuring of these data was examined using the Mantel test with respect to the corresponding spatial structuring for various environmental variables which might affect growth of the white sucker. These environmental variables include lake geographic location, lake morphometry, water chemistry, food supply, presence/absence of major predator species, and population density and length at sexual maturity of the white sucker. Geographic distances among lakes and among-lake differences in water chemistry were significantly related to among-population differences in length-at-age. Among-lake differences in lake morphometric variables, benthos densities, presence/absence of predator species, and length at maturity and population density of the white sucker were not significantly related to among-population differences in length-at-age. No sex-specific differences in the effects of environmental variables on length-at-age were observed. This study suggests that the among-lake differences in water chemistry (thus, physiological stresses) and isolation-by-distance (thus, genetic forces) are the two most important factors in patterning the large variations in length-at-age among white sucker populations. However, discerning the separate effect of each of these two factors is not possible because the spatial patterns of these two factors are related.

The presence of protease inhibitors in different ingredients used in fish feeds may negatively affect their digestive utilisation. The effect of inhibitors present in several protein sources on the activity of digestive proteases of sea bream was assessed using ‘in vitro’ assays. Inhibition produced by extracts of plant proteins ranged from 25 to 50% of total activity, whereas that obtained using animal protein sources ranged from 1 to 20 %. The exception was ovoalbumin, which showed the highest measured value (62 %). A plot of the inhibition values obtained by changing the relative concentrations enzyme/inhibitor resulted in different curves for different protein sources. The use of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) zymograms allowed visualisation of the aforementioned differences in inhibition. The importance of measuring protease inhibition in the preliminary evaluation of fish feed ingredients is discussed.

We propose a simple method to quantify the morphology of 2-3-year-old carps (Cyprinus carpio) which is related to their fillet yield. This method is based on the automated image analysis of the lateral projection of the fish (mask). Seven morphological measurements specifically related to fillet yield of carps were defined. The comparison between this method and the conventional quantification of fish shape, based on measurements of skeletal landmarks, increases the significance of this fitting. From the analysis of the morphometric data, we propose a profitable carp morphotype which is defined by four angles and two distances which quantify the shape of the head and the position of the caudal peduncle, respectively. The main characteristic of this morphotype is the absence of a large dorsal development, and the ventral position of the caudal peduncle.

The effect of different parameters on short-term storage capacity of turbot ova was assessed over a 45-h period after ova collection for fertilization rates and over a 9-h period after ova collection for hatching rates. Increasing the volume of ova sampling from 0.5 to 2.5 mL, as well as adding an antibiotic-antimicotic solution or oxygen did not significantly change the storage capacity of ova. Regarding the hatching rates, a higher storage ability was recorded at 8 and 13 °C, compared to 3 °C. The mean composition of the ovarian fluid was determined (n = 57 spawns). Use of a diluent mimicking the ovarian fluid significantly decreased the storage ability as assessed by the fertilization rates but did not modify the hatching rates. Diluting ova in an artificial ovarian fluid deprived of calcium significantly decreased the fertilization and hatching rates during the storage period. Furthermore, addition or not of soybean trypsin inhibitor (Sigma T 9003) to the artificial ovarian fluid deprived of calcium did not significantly change the results. Storage capacity of control batches of ova was low: at 13 °C, without any diluent and when ova were fertilized 3 h after stripping, the hatching rate was lowered to 62.4 ± 29.4 % (mean ± SD) of the initial value.

Despite its wide use in terrestrial ecology, distance sampling is as yet rarely used in underwater visual censuses. The present study attempts to compare density estimators based on distance sampling and on strip transects. Three stations with increasing densities of Chaetodontidae and Pomacanthidae were sampled twice by two divers of unequal experience, using two different transect types. A total of 96 transects and 2970 records of Chaetodontidae and Pomacanthidae were analysed. Nine estimators based on distance sampling were calculated and only the best fit (DT estimator) was kept for comparison with other estimators. These were either based on the average distance of the fish to the transect (AD estimator), or a 3-m- or 5-m-wide strip transect estimator (FW3 and FW5, respectively). There were no significant differences between the means found by DT, AD and FW3. Lower density estimates were given by FW5 in all cases. FW3 and FW5 did not detect several significant differences between stations which were otherwise detected by DT or AD. The number of transects needed to detect a significant difference between stations was four to ten times higher with FW3 or FW5 than with DT or AD. Diver experience was found to be a significant factor in density estimates. However, this factor was less important than the choice of the density estimator. Transect type or the day of sampling had no consequence for the estimates. The distance distributions of fish were divided into three different patterns which may be explained by a combination of detectability function and a behavioural component.

Onchocerciasis is a widespread disease in intertropical Africa, which, ultimately, causes irreversible blindness. The disease is transmitted by a small blackfly, Simulium damnosum (Diptera), which has aquatic larval and pupal stages. The breeding sites of the blackflies are riffles. These river reaches are the targets of the control campaign of the Onchocerciasis Control Programme in West Africa (OCP). An aquatic monitoring network covering the totality of the area exposed to the insecticide was set up to evaluate environmental impact. In this paper, we present results from the OCP 20-year period of monitoring of the ichthyofauna regularly exposed to larvicides. We do not record any measurable effects of pesticides on the CPUE, abundance of species, trophic structure, community structure or fish health. However, we detect the emergence of a number of medium-term tendencies. These tendencies may relate to climatic conditions that have a consequent effect on hydrology. Thus, we note a constant decrease in the CPUE from the beginning of the monitoring until 1995. The rivers were treated during that time until 1990 or 1993, depending on the station. But even after the treatments ended, the number of catches continued to decrease. As a result, we consider other factors to be the cause of that decline. The average level of annual discharges in this region has been decreasing regularly from the beginning of the 1970s. The production of fish fluctuates in all the rivers according to the flood rate. Important floods inundate larger areas, making greater quantities of food available, and thus improving the conditions for reproduction. The determining factor of the ichthyological stock abundance seems to depend both on the extent and the duration of the flood. In our catches, the observed effect was not immediately evident but appeared a few years later as a cumulative effect of poor hydrological conditions. An increase in the CPUE since 1996 has been related to improved hydrological conditions. In these last few years, we have observed an intensification of the basic flow leading to a ground water renewal. Furthermore, on three of the stations investigated, it appeared that the impediment of rivers (dams) could induce different and/or antagonistic effects. In some cases, we have observed that in spite of unfavourable hydrological conditions, certain species appear to be favoured by the presence of the dam. But, the damming of the river has a negative effect on other species, particularly on the coefficient of condition of migratory fishes. The impact of these factors is enhanced by the fact they exist conjointly.

Duplicate groups of first-feeding (4-5-d-old) Tilapia zillii (Gervais) were maintained on two feeding regimes (high and low ration size) for a total period of 550 d (~ 17 months) in environmentally-controlled aquaria. Differences in mean individual fish weight were evident between fish fed the different ration sizes after just 3 months, though these differences were not statistically significant until day 180. Thereafter, fish maintained upon the higher ration were significantly larger than fish fed a reduced ration. Mortality in high ration fish over the course of the experiment was ~60 % compared to ~88 % in low ration fish. Mortality in both treatment groups was most pronounced during the first three months (particularly during the first 30 d) but was much reduced thereafter. Spawning trials undertaken from day 450 onwards indicated that there was no significant difference between the two ration groups in terms of spawning periodicity over a discrete time period of 60 d. Mean total fecundity was significantly higher in fish on high rations though mean egg diameter and gonadosomatic index remained unchanged. Adjustment of spawning data to a common maternal fish size using one-factor ANCOVA failed to detect significant differences between the two ration levels in terms of either mean total fecundity or mean egg diameter. Stereological analysis of plastic-embedded ovarian tissue revealed that the ovaries of low ration fish possessed significantly more stage 2 and 3 (early and late perinucleolar) oocytes and significantly less stage 6/7 (late-vitellogenic/maturing) oocytes than the ovaries of fish fed the higher ration. No significant differences were detected between the two rations in terms of ovarian atresia. It is suggested that under prolonged food restriction, female T. zillii sacrifice somatic growth such that reproductive investment can be maintained.

Information on the distribution of the benthopelagic fauna of the southern Aegean sea was collected through two bottom-trawl surveys carried out at fixed sampling stations in the summers of 1996 and 1997. Discriminant analysis identified that the most important species in discriminating among station-groups in terms of numbers were: Pagellus erythrinus, Mullus barbatus, Mullus surmuletus, Diplodus annularis, Spicara flexuosa, Sardina pilchardus and Parapenaeus longirostris. In terms of weight, the most important discriminating species were: Mullus barbatus, Pagellus erythrinus, Spicara maena, Scyliorhinus canicula, Loligo vulgaris and Parapenaeus longirostris. Supervised machine learning approaches and, in particular, the decision tree construction method were utilized in order to induce rules which determine the station-grouping. Several species, notably, Argentina sphyraena, Aristaeomorpha foliacea, Lepidorhombus bosci, Lepidotrigla cavillone, Mullus barbatus, Serranus cabrilla and Sepia officinalis appeared in most of the rules.

The present paper suggests a method for estimating the fishing power of vessels and for analysing fleet dynamics. The approach is based on quantification of stocks catchability (q), derived from fishing mortality coefficients (F) as calculated by virtual population analysis. Catchabilities for each harvested stock are thus estimated relatively to the fishing effort (fn) of each vessel, according to the equation: q = (F/fn). A linear model is then fitted to these catchabilities. The model allows the identification and quantification of trends in average mortality rates per fishing hour for each stock. Under some assumptions, trends are interpreted as variations in the overall fishing power of each fleet. The approach is applied to three industrial and semi-industrial fleets of Brittany (Lorient, Concarneau and Douarnenez) and to the main gadoid stocks they exploit off the west coast of Scotland (ICES area VIa), and in the Celtic Sea (ICES area VIIf,g,h) for Concarneau. Results show large variations in fishing power. Particularly, a marked increase trend in the fishing power exerted on saithe (Pollachius virens) is highlighted for the three fleets, over the period 1983-1989. These variations can be explained by the redirection of fishing strategies, which may occur on a large scale. Thus, we show how the collapse of saithe stock at first led the three fleets to intensify the harvesting of saithe, and from 1989 on, to adopt different strategies. The possible causes of the observed dynamics are discussed, as well as their consequences for fisheries management. In particular, the relevance of direct control of fishing effort as a regulatory tool is questioned.

Egg quality is one of the most important factors determining larval viability. The study of oocytes released by ‘wild' broodstock will contribute to the definition of quality criteria applied to hatcheries as well as being an indicator of the oyster reproduction potential and aquaculture ongrowing systems. The aim of this study was to determine the relationship between condition of Crassostrea gigas spp. angulata (Portuguese strain) from ‘wild' broodstock (condition index, gonadal maturity and number of oocyte released), oocyte biochemistry and hatching rate of eggs during the spring/summer of 1995. Stages of gonadal maturity and condition index indicated that natural spawning of this species occurred between April and August with the highest spawning intensity occurring in June. Analyses demonstrated that C. gigas oocyte organic matter consist of 44–74 % proteins, 16–38 % lipids and 7–12 % carbohydrates. A significant relationship was observed between some biochemical parameters – organic matter and lipid content – and broodstock condition index and hatching rate. It seems that these two oocyte parameters (organic matter and total lipids) could be used to define quality of oocyte and thus larval viability. The oocyte protein content was also found to be associated with broodstock and larval quality. In summary, these data are a contribution to the knowledge of the reproductive cycle of oysters in Europe and may be useful in improving hatchery management of this species.

Fish catches around the Great Lake, Tonle Sap river and the transitional zone between the lake and the river were studied by professional ‘fishing lot' (i.e. fishing zone) from 1995 to 1997. One hundred and twenty species of fish, belonging to 26 families and nine different orders were recorded. Compared to previous studies (1936–1976), only 53 % of families, 32 % of genera and 54 % of species were collected by professional fishing lot. This important loss in biodiversity could have several causes: bias of sampling procedures between professional fishing and research sampling, overfishing, modification of the floodplain by deforestation, etc. The professional fishing data showed that Cypriniformes accounted for 41.6 % of the total number of individuals caught, the Cyprinidae family alone represented 40 species. Siluriformes made up 21.6 %, Perciformes 13.3 %, with six other orders represented by smaller numbers. Eighty-seven percent of the recorded species occur in both habitats depending on the season. Migration takes place from the Mekong river to the Great Lake through the Tonle Sap river at the beginning of the rainy season (May–October), and in the reverse direction at the start of the dry season (November–February). The majority of the species reproduce at the start of the rainy season (May–June) in the flooded plain and the forest floodplain of the Great Lake; the period, the place and the means of reproduction have not been closely studied, particularly for the Belontiformes, Clupeiformes, Synbranchiformes, Pleuronectiformes and Tetraodontiformes. Multivariate analysis of fishing data (November–February) shows three distinct communities: that of the lake (Perciformes and Siluriformes), of the river (Pleuronectiformes, Cypriniformes, Clupeiformes and Siluriformes), and of the transitional zone formed from the principal channel, the old river channel and the oxbow lakes (Cypriniformes, Siluriformes and Osteoglossiformes).

There are currently at least six commercial fisheries harvesting six different species of euphausiid, or krill: Antarctic krill (Euphausia superba), fished in the Antarctic; North Pacific krill (Euphausia pacifica), fished off Japan and off western Canada; Euphausia nana, fished off the coast of Japan; Thysanoessa inermis, fished off the coast of Japan and off eastern Canada; and Thysanoessa raschii and Meganyctiphanes norvegica which have been experimentally harvested off eastern Canada. The current world catch of all species of krill is over 150 000 tonnes per annum but few fisheries are being exploited to their maximum theoretical potential. The size of the world krill harvest is currently limited by lack of demand, although some fisheries are being deliberately managed at low levels because of ecological concerns. We have outlined the history of these krill fisheries to determine where there are common trends and issues which will affect their future development. Krill products are currently mostly used for the aquaculture and sport fishing market but considerable effort has also been put into developing products for human consumption, partiularly from Antarctic krill. The future development of krill fisheries is examined in the light of information on trends in krill products which include pharmaceutical and industrial uses in addition to nutritional products. Because of the central ecological role of krill in many marine ecosystems, the subject of krill harvesting is a sensitive issue and krill fisheries require careful management. This requirement has spawned an innovative international management regime in the Antarctic - the Convention for the Conservation of Antarctic Marine Living Resources (CCAMLR) - which has developed procedures for managing the harvest of Antarctic krill that may be applicable to other fisheries. The common problems of managing krill fisheries are outlined particularly those relating to the role of krill in many coastal ecosystems, and as prey for many other species which are commercially fished.

The common dentex (Dentex dentex) is a sparid fish which is considered a suitable candidate for Mediterranean aquaculture. A seven-weeks feeding trial was conducted over the summer period with common dentex, sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax). All three species were fed to visual satiety with two practical diets with varying crude protein and crude fat levels (55 % protein, 9 % lipids; 46 % protein, 17 % lipids). The initial body weights were 8.2, 11.6 and 17.3 g for common dentex, sea bass and sea bream, respectively. In all cases, specific growth rates were not significantly affected by dietary treatment, but they varied among species (sea bass 1.7-1.8 %; sea bream 2.1-2.3 %; common dentex 3.1-3.2 %). When comparisons between fish species were made, we observed that the increase in growth rates was linked to a decrease of the whole body fat content, as a consequence of a greater utilisation of dietary lipids as energy substrates. Furthermore, in common dentex, the 17 % lipid diet was able to improve protein retention, but this diet effect was less significant in sea bream and sea bass. In contrast to sea bream, the 17 % lipid diet did not up-regulate plasma GH levels in common dentex, which provides additional evidence for a more efficient utilisation of dietary lipids.

The relationship between serum protein, immunoglobulin concentrations and protein molecular weight profile (PMWP) alterations of sea bass (131.3 ± 4.3 g) reared in sea water with sublethal concentrations of ammonia was studied over two periods totalling 116 days. During the exposure period (62 days) the first group (group 1) lived in sea water with 0.204 mg·L−1 unionized ammonia nitrogen equivalent to 12 % of the lethal concentration for 50 % of a population exposed for 96 h to ammonia (96-h LC50), whilst the second group (group 2) lived in sea water with 0.340 mg·L−1 UIA-N equivalent to 20 % of the 96-h LC50 of ammonia. Then, the two groups were left for a recovery period (54 days) in the same water as the control group (group 0). The determination of the total immunoglobulin (Ig) concentration was carried out by enzyme-linked immunosorbant assay (ELISA). Gel filtration columns were used for the serum PMWP. Serum Ig concentration of the exposed fish (group 2: 1.76 ± 0.43 mg·mL−1; group 1: 1.19 ± 0.33 mg·mL−1) was lower than the control group 13.39 ± 1.01 mg·mL−1) after 21 days of exposure period but this difference was reduced at the end of this period (day 62) and treated group Ig concentrations switched to higher than the control group after the recovery period (day 116, group 2: 9.75 ± 1.84 mg·mL−1; group 1: 7.50 ± 1.22 mg·mL−1 group 0: 6.38 ± 1.13 mg·mL−1). In fact, at the end of the experiment, the cumulative Ig production difference between fish exposed to ammonia and the control was less than 10%. Although a similar evolution of the Ig serum occurred with protein concentration, the serum protein concentration deficit of group 1 was restored at the end of the exposure period (group 2: 46.49 ± 2.34 mg·mL-1; group 0: 46.74 ± 1.97 mg·mL−1) and the cumulative production during the experiment was not significantly different between group 1 and group 0. However, this remained lower for group 2. During the exposure period, the PMWP of treated fish moved towards smaller molecular weight proteins. This alteration of the PMWP showed that the 0.2-kDa fraction increased, and another fraction appeared at the end of the exposure period. However, at the end of the recovery period, no difference was found between the PMWPs of control and treated fish. The alterations of the serum protein and Ig of fish reared, for a limited period (2 months), in water with sublethal concentrations of ammonia (20 % 96-h LC50 or less) should disappear completely after a few months in normal rearing conditions.

Two trawl surveys were carried out in the summer months (June–July) of 1996 and 1997 along the whole South Adriatic area (Mediterranean sea) for the first time, using the same vessel and the same sampling gear (European Community Research Project ‘MEDITS’); previous research data referred only to the south-western Adriatic side. A total list of 168 demersal species (fishes, cephalopods and crustaceans) was obtained during the surveys; species abundance data (individuals/trawling hour) were processed according to multivariate techniques in order to describe the composition and the distribution of the main species assemblages within the investigated area (10–800-m bathymetric range). Multivariate analysis of MEDITS survey catch data showed a bold relationship between fish assemblages and depth, while the influence of depth was lower with respect to cephalopod and especially crustacean assemblage distribution.

The French oyster farming industry relies almost exclusively on juveniles collected from the natural environment; the supply of spat produced by hatcheries is low, about 10 % of the industry's requirements. Development through selective breeding of oyster stocks which are better suited for aquaculture purposes, is likely to reverse this tendency since only hatcheries will be able to supply such animals. Scallop farming, which at present is poorly developed in France, relies exclusively on hatchery produced spat. Although hatchery technology is constantly being improved, significant production problems remain which must be solved before hatcheries become a major supplier of juveniles for the industry. This paper describes the present state of hatchery technology in France based on experimental results obtained with the great scallop Pecten maximus and the Pacific oyster Crassostrea gigas, over the past ten years. Compared to the great scallop, the Pacific oyster has higher D larva yields (60 % for C. gigas vs. 30 % for P. maximus) and a faster larval growth rate (10 μm·d−1 for C. gigas vs. 5 μm·d−1 for P. maximus). However, Pacific oysters have a greater heterogeneity during larval development and higher mortality rates (40 % for C. gigas vs. 25 % for P. maximus) which produce lower overall yields of pediveliger larvae ready-to-set for Pacific oysters (15 %) compared to the great scallop (30 %). Development of continuous larval and post-larval culture methods along with development of continuous phytoplankton technology offers one of the most promising methods to improve molluscan hatchery techniques.

Larvae of the coral reef damselfish Acanthochromis polyacanthus (Bleeker) were fed either unenriched Artemia nauplii or nauplii which had been enriched by pre-feeding with microcapsules containing either squid oil (SQO) or cod liver oil (CLO). Enriched nauplii contained elevated levels of the n-3 highly unsaturated fatty acids (HUFA) eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) which made up 5.22 ± 0.34 and 2.62 ± 0.28 %, respectively, of total fatty acids in nauplii enriched with CLO, and 10.48 ± 0.36 and 3.43 ± 0.33 %, respectively, in nauplii enriched with SQO. In contrast, unenriched nauplii contained EPA (5.03 ± 1.04 %) but did not contain DHA. Survival differed significantly between treatments over the 18-d study; larvae receiving CLO enriched nauplii showed 100 % survival and those receiving SQO enriched nauplii showed 93.3 ± 6.6 % survival. In contrast, only 46 ± 6.7 % of larvae receiving unenriched nauplii survived to the end of the 18-d study. Wet weight, dry weight and proximate biochemical composition did not differ significantly between treatments at the end of the study. Mean standard length of larvae fed CLO enriched nauplii was significantly smaller than that of larvae fed SQO enriched nauplii; however, neither differed significantly from larvae fed unenriched nauplii. The fatty acid composition of A. polyacanthus larvae was significantly influenced by the fatty acid composition of the diet. The results indicate that A. polyacanthus larvae are unable to synthesise DHA from available dietary precursors and, as such, dietary DHA is required to maximise survival. Development of appropriate culture techniques for the larvae of coral reef fishes will allow controlled laboratory studies with these species and may eventually reduce pressure on wild populations exploited for the aquarium trade.