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Population genetic studies on the Australian freshwater crayfish, Cherax destructor (Crustacea: Parastacidae) using allozyme and RAPD markers

Published online by Cambridge University Press:  15 March 2005

Thuy T. T. Nguyen ,
Christopher P. Burridge  and
Christopher M. Austin
Thuy T. T. Nguyen
Affiliation:
School of Ecology and Environment, Deakin University, Warrnambool, VIC 3280, Australia Network of Aquaculture Centres in Asia-Pacific, PO Box 1040, Kasetsart Post Office, Bangkok 10903, Thailand
Christopher P. Burridge
Affiliation:
School of Ecology and Environment, Deakin University, Warrnambool, VIC 3280, Australia
Christopher M. Austin
Affiliation:
School of Ecology and Environment, Deakin University, Warrnambool, VIC 3280, Australia
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Abstract

Allozyme and Random Amplified Polymorphic DNA (RAPD) variation was surveyed in the freshwater crayfish Cherax destructor Clark, an ecologically and commercially important species that is widespread throughout the freshwater systems of central Australia. At the intra-population level, allozymes revealed a similar level of variation to that found in other freshwater crayfish; RAPDs showed less diversity than allozymes, which was unexpected. At the inter-population level, both techniques revealed significant population structure, both within and between drainages. RAPD results were consistent with phylogeographic patterns previously identified using mtDNA. Although allozyme data showed little geographic pattern in relation to genetic variation based on multidimensional-scaling (MDS) plots on matrices of genetic distance, results of AMOVA and Mantel tests indicated significant population structuring. Each of the mtDNA lineages proposed in a previous study also showed significant genetic structure at similar levels as revealed by RAPDs but different levels by allozymes. These results reject hypotheses previously put forward on genetic homogenisation within the species due to wide-scale translocation. The implications of the findings for conservation and aquaculture of C. destructor are also discussed.

Keywords

Population geneticsAllozymesRAPDsCherax destructor
Type
Research Article
Information
Aquatic Living Resources , Volume 18 , Issue 1 , January 2005 , pp. 55 - 64
Copyright
© EDP Sciences, IFREMER, IRD, 2005

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