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Polymorphism of major histocompatibility complex class II B genes in different lines of the common carp (Cyprinus carpio)

Published online by Cambridge University Press:  15 October 2003

Krzysztof Ł. Rakus*
Affiliation:
Institute of Ichthyobiology and Aquaculture in Golysz, Polish Academy of Sciences, ul. Kalinowa 2, 43520, Chybie, Poland Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH, Wageningen, The Netherlands
Geert F. Wiegertjes
Affiliation:
Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH, Wageningen, The Netherlands
René J. M. Stet
Affiliation:
Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH, Wageningen, The Netherlands
Huub F. J. Savelkoul
Affiliation:
Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH, Wageningen, The Netherlands
Andrzej Pilarczyk
Affiliation:
Institute of Ichthyobiology and Aquaculture in Golysz, Polish Academy of Sciences, ul. Kalinowa 2, 43520, Chybie, Poland
Ilgiz Irnazarow
Affiliation:
Institute of Ichthyobiology and Aquaculture in Golysz, Polish Academy of Sciences, ul. Kalinowa 2, 43520, Chybie, Poland
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Abstract

Regular observation of survival of the carp breeding lines constituting a living gene bank at the Institute of Ichthyobiology and Aquaculture in Golysz (Poland) over a period of at least 15 years showed different survival rates for various lines. In this study, we have examined the polymorphism of the major histocompatibility complex (MHC) gene class II B in nine carp lines. The class II B gene encodes for the part of the MHC class II molecule which presents peptides from pathogens and protein antigens that are present in the extracellular milieu and have been taken up into the endocytic vesicles of antigen-presenting cells. Polymerase chain reaction was used to amplify Cyca-DAB gene fragments comprising part of exon 1, complete intron 1 and almost complete exon 2. Exon 2 encodes for the β1 domain which is the most polymorphic fragment of MHC class II molecules. Single-strand conformational polymorphism (SSCP) was applied to detect different MHC class II B haplotypes. The analysis revealed the presence of seven different haplotypes occurring with various frequencies.

Type
Research Article
Copyright
© Elsevier, IRD, Inra, Ifremer, Cemagref, 2003

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