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Isolation and characterization of fifteen microsatellite locifrom the redclaw crayfish, Cherax quadricarinatus

Published online by Cambridge University Press:  19 July 2010

Yannan Xie ,
Lin He ,
Jiangling Sun ,
Lili Chen ,
Yunlong Zhao ,
Yaping Wang  and
Qun Wang
Yannan Xie
Affiliation:
School of Life Science, East China Normal University, Shanghai 200062, China
Lin He
Affiliation:
School of Life Science, East China Normal University, Shanghai 200062, China
Jiangling Sun
Affiliation:
School of Life Science, East China Normal University, Shanghai 200062, China
Lili Chen
Affiliation:
School of Life Science, East China Normal University, Shanghai 200062, China
Yunlong Zhao
Affiliation:
School of Life Science, East China Normal University, Shanghai 200062, China
Yaping Wang
Affiliation:
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China
Qun Wang*
Affiliation:
School of Life Science, East China Normal University, Shanghai 200062, China
*
b Corresponding author: [email protected]
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Abstract

The redclaw crayfish (Cherax quadricarinatus) is a freshwater crayfishspecies endemic to northern Australia and Papua New Guinea that is the focus of a growingculture industry in number of regions around the world. Here we isolated and characterized15 microsatellite loci from Cherax quadricarinatus and tested variabilityof the loci in 66 unrelated cultured individuals. Thirteen of 15 microsatellite loci werepolymorphic. Number of alleles per locus ranged from two to seven while observed andexpected heterozygosities ranged from 0.172 to 0.985 and from 0.373 to 0.778,respectively. Eleven loci conformed to Hardy–Weinberg equilibrium in the sampledpopulation. These microsatellite loci developed here provide an important resource forstudying genetic diversity and population structure in redclaw crayfish and potentially inother related species.

Keywords

AquacultureMicrosatellitesDNA sequencesGenetic diversityPopulation structureDecapod CrustaceaCherax quadricarinatus
Type
Note
Information
Aquatic Living Resources , Volume 23 , Issue 2 , April 2010 , pp. 231 - 234
Copyright
© EDP Sciences, IFREMER, IRD 2010

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