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Induced spawning of red drum, Sciaenops ocellatus : use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality

Published online by Cambridge University Press:  15 January 2000

Lionel Gardes ,
Philippe Villanove ,
Vincent Buchet  and
Christian Fauvel
Lionel Gardes
Affiliation:
Laboratoire ressources aquacoles, Institut français pour l'exploitation de la mer, Pointe Fort, 97231 Le Robert Martinique, France
Philippe Villanove
Affiliation:
Station expérimentale d'aquaculture, Institut français pour l'exploitation de la mer, Chemin de Maguelonne, 34250 Palavas, France
Vincent Buchet
Affiliation:
Station expérimentale d'aquaculture, Institut français pour l'exploitation de la mer, Chemin de Maguelonne, 34250 Palavas, France
Christian Fauvel
Affiliation:
Station expérimentale d'aquaculture, Institut français pour l'exploitation de la mer, Chemin de Maguelonne, 34250 Palavas, France
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Abstract

The present work examines the usefulness of the combination of multivariate analyses and further one-way analysis of variance in the search for links between causes and effects in heterologous stimulation of spawning in fish. Stimulations with both 20 and 50 μg.kg–1 doses of [D Trp6] luteinizing hormone-releasing hormone analogue (LH-RHa) were effective in inducing and synchronizing spawning of red drum, Sciaenops ocellatus. Oocyte development monitoring using ovarian biopsy allowed treatment injections at different stages of maturation. The influence at stimulation time of female oocyte development state upon further development was shown. An interesting potential of second stimulation, leading to high quality spawns, was established and factors of success were identified. The best conditions for stimulation success were simultaneously late stage of oocyte development characterized by the beginning of oil droplet coalescence, high ovarian homogeneity quoted by the proportion of most developed oocytes in the biopsy sample, and low dose of hormone (20 μg.kg–1) for both first and second stimulations.

Keywords

Fish cultureinduced breedingLH-RH analoguesmultivariate analysisegg qualityoocyte developmentred drumSciaenops ocellatus
Type
Research Article
Information
Aquatic Living Resources , Volume 13 , Issue 1 , January 2000 , pp. 19 - 27
Copyright
© Elsevier, Inra, Ifremer, Cemagref, Ird, Cnrs, 2000

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