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Genotoxic and CYP 1A enzyme effects consecutive to the food transfer of oil spill contaminants from mussels to mammals

Published online by Cambridge University Press:  15 October 2004

Sébastien Lemière ,
Carole Cossu-Leguille ,
Sylvie Chaty ,
François Rodius ,
Antonio Bispo ,
Marie-José Jourdain ,
Marie-Claire Lanhers ,
Daniel Burnel  and
Paule Vasseur
Sébastien Lemière
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
Carole Cossu-Leguille
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
Sylvie Chaty
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
François Rodius
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
Antonio Bispo
Affiliation:
IRH Environnement, 11 bis rue G. Peri, BP 286, 54515 Vandœuvre-les-Nancy, France
Marie-José Jourdain
Affiliation:
IRH Environnement, 11 bis rue G. Peri, BP 286, 54515 Vandœuvre-les-Nancy, France
Marie-Claire Lanhers
Affiliation:
INSERM U420, Faculté de Médecine, 9 Av. Forêt de Haye, 54505 Vandœuvre-les-Nancy, France
Daniel Burnel
Affiliation:
INSERM U420, Faculté de Médecine, 9 Av. Forêt de Haye, 54505 Vandœuvre-les-Nancy, France
Paule Vasseur
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
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Abstract

The transfer of polycyclic aromatic hydrocarbons (PAHs) from bivalves, through the food chain to vertebrates was of concern. Our research aimed at estimating potential effects for consumers resulting from the ingestion of seafood contaminated by oil spill pollutants.After the “Erika” wreck, mussels (Mytilus sp.) were collected from sites of the Atlantic coast impacted to various degrees by the oil slick and constituted contaminated food for rats during 2 and 4 weeks. Genotoxic damage were studied in rats by means of COMET assay carried out in liver, bone marrow and peripheral blood. Biochemical and genomic effects such as the induction of CYP 1A1 and the expression of cytochrome genes were measured in rat livers. The most sensitive biological parameter reflecting the transfer of contaminants via the food appeared to be DNA breaks studied by means of the COMET assay. Genotoxic damage, observed mainly in the liver, were rather moderate and remained not persistent. This study underlined the bioavailability of pollutants in fuel oil contaminated mussels for consumers, and the complexity of the contamination consecutive to the oil spill. The occurrence of related PAH compounds in addition to non-substituted PAHs in fuel oils and mussels raised the question of their implication in the registered effects.

Keywords

Polycyclic aromatic hydrocarbonsOil spillCOMET assayDNA damageEROD activityCYP 1AFood transfer
Type
Research Article
Information
Aquatic Living Resources , Volume 17 , Issue 3: The "Erika" Oil Spill: Environmental Contamination and Effects in the Bay of Biscay , July 2004 , pp. 303 - 307
Copyright
© EDP Sciences, IFREMER, IRD, 2004

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