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Genomic sequences and genetic differentiation at associatedtandem repeat markers in growth hormone, somatolactin and insulin-like growth factor-1genes of the sea bass, Dicentrarchus labrax

Published online by Cambridge University Press:  04 October 2010

Nolwenn Quéré ,
Bruno Guinand ,
Heiner Kuhl ,
Richard Reinhardt ,
François Bonhomme  and
Erick Desmarais
Nolwenn Quéré
Affiliation:
Université Montpellier 2, Biologie intégrative, Institut des Sciences de l’Evolution de Montpellier, CNRS-UMR 5554, CC 63, 34095 Montpellier Cedex 5, France
Bruno Guinand*
Affiliation:
Université Montpellier 2, Biologie intégrative, Institut des Sciences de l’Evolution de Montpellier, CNRS-UMR 5554, CC 63, 34095 Montpellier Cedex 5, France Station Méditerranéenne de l’Environnement Littoral, 1 quai de la Daurade, 34200 Sète, France
Heiner Kuhl
Affiliation:
Max-Planck-Institute Molecular Genetics , Ihnestrasse 63-73, 14195 Berlin-Dahlem, Germany
Richard Reinhardt
Affiliation:
Max-Planck-Institute Molecular Genetics , Ihnestrasse 63-73, 14195 Berlin-Dahlem, Germany
François Bonhomme
Affiliation:
Université Montpellier 2, Biologie intégrative, Institut des Sciences de l’Evolution de Montpellier, CNRS-UMR 5554, CC 63, 34095 Montpellier Cedex 5, France Station Méditerranéenne de l’Environnement Littoral, 1 quai de la Daurade, 34200 Sète, France
Erick Desmarais
Affiliation:
Université Montpellier 2, Biologie intégrative, Institut des Sciences de l’Evolution de Montpellier, CNRS-UMR 5554, CC 63, 34095 Montpellier Cedex 5, France
*
a Corresponding author:[email protected]
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Abstract

The completion of genomic sequences of physiologically important genes frequently revealsnon-coding genetic elements such as tandem repeats (micro- and minisatellites) that areoften more polymorphic than nearby coding sequences. We obtained the complete genomicsequences of three hormone genes in sea bass Dicentrarchus labrax: growthhormone (dlGH), somatolactin (dlSL) and insulin-likegrowth factor-1 (dlIGF-1), including 5′- and 3′-untranslated regions.Mini- and microsatellites were discovered in both flanking and intron regions. Some werepartially conserved across Perciformes. To assess the usefulness and relevance of thesegene-associated markers for understanding population structure, an investigation was madeon genetic diversity and differentiation at four of them in (i) five wildpopulations from the North Sea, the Bay of Biscay and the Western Mediterranean, and(ii) two samples of hatchery-bred individuals from afreshwater-acclimation experiment. Gene and allelic diversities were lower in culturedindividuals than in wild ones. Significant genetic differentiation was demonstratedbetween Bay of Biscay + North Sea and Mediterranean populations(F st > 0.06, p < 0.001),primarily due to dlGH-associated markers. Significant geneticdifferentiation was also detected among the Atlantic and North Sea samples, but restrictedto the locus associated with dlSL. Significant genetic differentiationwas also found among experimental individuals before and after a salinity challenge(F st ≈ 0.05, p < 0.001), but was dueto dlSL and dlIGF-1 loci. Gene-associated markers provedto be more efficient than formerly used anonymous microsatellite markers in providing aclear picture of genetic differentiation.

Keywords

Growth hormoneSomatolactinInsulin-like growth factor-1Non-coding regionsEuropean sea bassMicrosatellitesMinisatellites
Type
Research Article
Information
Aquatic Living Resources , Volume 23 , Issue 3 , July 2010 , pp. 285 - 296
Copyright
© EDP Sciences, IFREMER, IRD 2010

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