Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-22T18:07:04.489Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of some hemato-immunological parameters in the mangrove oyster Crassostrea rhizophorae of different habitats of Santa Catarina Island, Brazil

Published online by Cambridge University Press:  15 July 2005

Tania Barth ,
Neci Moraes  and
Margherita Anna Barracco
Tania Barth
Affiliation:
Laboratório de Imunologia Aplicada à Aqüicultura, Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas (CCB), Universidade Federal de Santa Catarina (UFSC), CP 476, Florianópolis, SC 88040-900, Brazil
Neci Moraes
Affiliation:
Laboratório de Histologia, Departamento de Morfologia, CCB, UFSC, Brazil
Margherita Anna Barracco
Affiliation:
Laboratório de Imunologia Aplicada à Aqüicultura, Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas (CCB), Universidade Federal de Santa Catarina (UFSC), CP 476, Florianópolis, SC 88040-900, Brazil
Get access

Abstract

The main purpose of this study was to establish the pattern of variability of some hemato-immunological parameters in the mangrove oyster, Crassostrea rhizophorae, from 3 different environmental conditions in Santa Catarina Island: two natural habitats (mangrove and rocks of the coastal bay) and one oyster culture station. The water quality was adequate in all localities. The animals were collected seasonally over a period of one year (n = 30, per locality). The oyster hemolymph comprised two basic cell populations, hyaline (HH) and granular hemocytes (GH). Both cell populations contained carbohydrates and glycogen in their cytoplasm (periodic acid Schiff reaction, PAS staining) and the occurrence of lysosomes was suggested by the detection of acid phosphatase (Gomori's method). Both hemocyte populations were able of phagocytosis of zymosan particles in vitro and producing cytotoxic molecules, such as the superoxide anions (nitroblue-tetrazolium, NBT reduction). The oyster hemograms significantly differed in the different habitats and also among seasons. The total circulating hemocyte counts (THC) and the percentage of GH in the mangrove and rock oysters always differed from each other, but not from those of the cultured oysters. The highest THC and the lowest percentage of GH were always found in summer. The total plasma protein concentration (PC) exhibited a similar seasonal pattern in all oyster populations, with a marked decrease in spring. The PC of the cultured oysters was almost always significantly lower than that of the other oyster populations. The plasma agglutinating titer was higher in the cultured oysters regardless of season. The histological organization of the oyster digestive gland and gills was basically similar to that of other oyster species. Their structural aspect did not show any detectable alteration, corroborating that the oysters were in good health. The results of this study will serve as a basis for further analyses on the monitoring of C. rhizophorae health status and environmental quality in different aquatic habitats.

Keywords

MollusksBivalvesCrassostrea rhizophoraeHemato-immunological parametersHemolymphHemocytesDigestive glandGills
Type
Research Article
Information
Aquatic Living Resources , Volume 18 , Issue 2 , April 2005 , pp. 179 - 186
Copyright
© EDP Sciences, IFREMER, IRD, 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anderson, R.S., 1994, Hemocyte-derived reactive oxygen intermediate production in 4 bivalve mollusks. Dev. Comp. Immunol. 18, 89-96. CrossRef
Anderson R.S., 1996, Production of oxygen intermediates by invertebrate hemocytes: Immunological significance. In: Söderhäll K., Iwanaga S., Vasta G.R. (Eds.), New directions in invertebrate immunology, pp. 109-129. SOS Publications, Fair Haven.
Auffret, M., 1989, Comparative study of the hemocytes of two oyster species: The European flat oyster, Ostrea edulis, Linnaeus 1750 and the Pacific oyster, Crassostrea gigas (Thunberg, 1793). J. Shellfish Res. 8, 367-373.
Austin, K., Paynter, K.T., 1995, Characterization of the chemiluminescence measured in hemocytes of the Eastern oyster, Crassostrea virginica. J. Exp. Zool. 273, 461-471. CrossRef
Azevedo, C., Villalba, A., 1991, Extracellular giant rickettsiae associated with bacteria in the gill of Crassostrea gigas (Mollusca, Bivalvia). J. Invertebr. Pathol. 58, 75-81. CrossRef
Bachère, E.D.C., Chagot, D., Grizel, H., 1988, Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation. Dev. Comp. Immunol. 12, 549-559. CrossRef
Bachère, E.D.C., Hervio, D., Mialhe, E., 1991, Luminol-dependent chemiluminescence by hemocytes of two marine bivalves, Ostrea edulis and Crassostrea gigas. Dis. Aquat. Org. 11, 173-180. CrossRef
Bancroft J.D., Stevens A., 1982, Theory and practice of histological techniques, 2nd ed. Churchill Livingstone, Edimburg.
Barracco, M.A., Medeiros, I.D., Moreira, F.M., 1999, Some hemato-immunological parameters in the mussel Perna perna. Fish Shellfish Immunol. 9, 387-404. CrossRef
Bayne B.L., Brown D.A., Burns K., Dixon D.R., Ivanovici A., Livingstone D.R., Lowe D.M., Moore M.N., Stebbing A.R.D., Widdows J., 1985, The effects of stress and pollution on marine animals, Praeger Publishers, New York, NY.
Bradford, M.M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72, 248-254. CrossRef
Bramble, L.H., Anderson, R.S., 1999, Lack of involvement of reactive oxygen species in the bactericidal activity of Crassostrea virginica haemocytes in contrast to Morone saxatilis phagocytes. Fish Shellfish Immunol. 9, 109-123. CrossRef
Brereton, J.D., Alderman, D.J., 1979, Wound healing in the European oyster, Ostrea edulis L. Aquaculture 16, 147-151. CrossRef
Cajaraville, M.P., Olabarrieta, I., Marigomez, I., 1996, In vitro activities in mussel hemocytes as biomarkers of environmental quality: A case study in the Abra Estuary (Biscay Bay). Ecotoxicol. Environ. Saf. 35, 253-260. CrossRef
Chu, E.F, La Peyre, J.F., 1993, Development of disease caused by the parasite Perkinsus marinus and defense-related hemolymph factors in 3 populations of oysters from the Chesapeake Bay, USA. J. Shellfish Res. 12, 21-27.
Coles, J.A., Farley, S.R., Pipe, R.K., 1995, Alteration of the immune response of the common marine mussel Mytilus edulis resulting from exposure to cadmium. Dis. Aquat. Organ. 22, 59-56. CrossRef
Eble A.F., Scro R., 1996, General Anatomy. In: Kennedy V.S., Newell R.I.E., Eble A.F. (Eds.), The Eastern Oyster: Crassostrea virginica, pp. 19-74. Maryland Sea Grant College, University of Maryland.
Elston R.A., 1990, Mollusc diseases: Guide for the shellfish farmer, pp. 37-39. University of Washington Press, Seattle.
Fisher, W.S., Oliver, L.M., Edwards, P., 1996, Hematologic and serologic variability of eastern oysters from Apalachicola Bay, Florida. J. Shellfish Res. 15, 555-564.
Fisher, W.S., Oliver, L.M., Winstead, J.T., Long, E.R., 2000, A survey of oysters Crassostrea virginica from Tampa Bay, Florida: associations of internal defense measurements with contaminant burdens. Aquat. Toxicol. 51, 115-138. CrossRef
Ford, S.E., Kanaley, S.A., Ashton-Alcox, K.A., 1989, In vitro recognition and phagocytosis of the oyster pathogen MSX. J. Shellfish Res. 8, 468.
Galtsoff P.S., 1964, The American oyster, Crassostrea virginica Gmelin. U.S. Fish Widl. Serv. Fish. Bull. 64,1-480.
Hawkins, W.E, Howse, H.D., 1982, Ultrastructure of cardiac hemocytes and related cells in the oyster Crassostrea virginica. Trans. Am. Microsc. Soc. 101, 241-252. CrossRef
Hégaret, H., Wikfors, G.H., Soudant, P., Delaporte, M., Alix, J.H., Smith, B.C., Dixon, M.S., Quére, C., Le Coz, J.R., Paillard, C., Moal, J., Samain, J.-F., 2004, Immunological competence of eastern oysters Crassostrea virginica, fed different microalgal diets and challenged with a temperature elevation. Aquaculture 234, 541-560. CrossRef
Hine, P.M., 1999, The inter-relationships of bivalve hemocytes: Review. Fish Shellfish Immunol. 9, 367-385. CrossRef
Howard D.H, Smith C.S., 1983, Histological techniques for marine bivalve mollusks. NOAA Tech. Memo. NMFS-F/NEC-25, pp. 97. Woods Hole, Massachusetts.
Lacoste, A., Malham, S.K., Gélébart, F., Cueff, A., Poulet, S.A., 2002, Stress-induced immune changes in the oyster Crassostrea gigas. Dev. Comp. Immunol. 26, 1-9. CrossRef
Nascimento, I.A., Smith, D.H., Kern, F.I., Pereira, S.A., 1986, Pathological findings in Crassostrea rhizophorae from Todos-os-Santos Bay, Bahia, Brazil. J. Invertebr. Pathol. 47, 340-349. CrossRef
Nirchio, M., Pérez, J.E., 1997, Changes in tissue water, leucine aminopeptidase activity, ninhidrine positive substances and protein concentration in Crassostrea rhizophorae exposed to extreme salinities. Bol. Inst. Oceanogr. Venezuela. 36, 31-36.
Olafsen, J.A., Fletcher, T.C., Grant, P.T., 1992, Agglutinin activity in Pacific oyster (Crassostrea gigas) hemolymph following in vivo Vibrio anguillarum challenge. Dev. Comp. Immunol. 16, 123-138. CrossRef
Ostini S., Poli C.R., 1990, A situação do cultivo de moluscos no Brasil. In: Hernandez R.A. (ed). Cultivo de moluscos na América Latina, Bogotá.
Oubella, R., Maes, P., Paillard, C., Auffret, M., 1993, Experimentally-induced variation in hemocyte density for Ruditapes philippinarum and R. decussatus (Mollusca: bivalvia). Dis. Aquat. Org. 15, 193-197. CrossRef
Oubella, R., Maes, P., Allam, B., Paillard, C., Auffret, M., 1996, Selective induction of hemocytic response in Ruditapes philippinarum (Bivalvia) by different species of Vibrio (Bacteria). Aquat. Living Resour. 9, 137-143. CrossRef
Paillard, C., Ashton-Alcox, K.A., Ford, S.E., 1996, Changes in bacterial densities and hemocyte parameters in eastern oysters, Crassostrea virginica, affected by juvenile oyster disease. Aquat. Living Resour. 9, 145-158. CrossRef
Pipe, R.K., Coles, J.A., Thomas, M.E., Fossato, V.U., Pulsford, A.L., 1995, Evidence for environmentally derived immunomodulation in mussels from the Venice Lagoon. Aquat. Toxicol. 32, 59-73. CrossRef
Roch, P., 1999, Defense mechanisms and disease prevention in farmed marine invertebrate. Aquaculture 172, 125-145. CrossRef
Romestand, B., Corbier, F., Roch, P., 2002, Protease inhibitors and haemagglutinins associated with resistance to the protozoan parasite, Perkinsus marinus, in the Pacific oyster, Crassostrea gigas. Parasitology 125, 323-329. CrossRef
Seiler, G.R., Morse, M.P., 1988, Kidney hemocytes of Mya arenaria (Bivalvia): normal and pollution-related ultrastructural morphologies. J. Invertebr. Pathol. 52, 201-214. CrossRef
Shumway, S.E., 1977, Effect of salinity fluctuation on the osmotic pressure and Na+, Ca2+ and Mg2+ ion concentrations in the hemolymph of bivalve molluscs. Mar. Biol. 41, 153-177. CrossRef
Sindermann, C.J., 1984, Disease in marine aquaculture. Helgoländer Meeresunters. 37, 505-532. CrossRef
Smith M.T., Evans C.G., Thor H., Orrenius S., 1985, Quinone-induced oxidative injury to cells and tissues. In: Sies H. (Ed.) Oxidative Stress, pp. 91-133. Academic Press, New York.
Vargas-Albores F., Barracco M.A., 2001, Mecanismos de defensa de los moluscos bivalvos, con énfasis en pectínidos. In: Maeda-Martinez, A.N. (Ed.) Los moluscos pectinidos de Iberoamerica: Ciencia y Acuicultura, pp. 127-146. Editorial Limusa, México.
Villalba, A., Azevedo, C., Rodríguez, C., 1993, Occurrence of multiple hyperplastic growths on the gills of Pacific oyster, Crassostrea gigas, and their relationship with associated pathologic conditions. J. Invertebr. Pathol. 61, 296-302. CrossRef
Volety, A.K., Chu, F.L.E., 1995, Suppression of chemiluminescence of eastern oyster (Crassostrea virginica) hemocytes by the protozoan parasite Perkinsus marinus. Dev. Comp. Immunol. 19, 135-142. CrossRef