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Effect of sublethal concentrations of copper sulphateon seabreamSparus aurata fingerlings

Published online by Cambridge University Press:  27 October 2007

Inmaculada Varo ,
Bruno Nunes ,
Francisco Amat ,
Amparo Torreblanca ,
Lúcia Guilhermino  and
Juan C. Navarro
Inmaculada Varo
Affiliation:
Departamento de Biología Funcional y Antropología Física, Facultad de Ciencias Biológicas, Universidad de Valencia, C/ Doctor Moliner s/n, Burjasot, Valencia, Spain
Bruno Nunes
Affiliation:
ICBAS – Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Departamento de Estudos de Populações, Laboratório de Ecotoxicología, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal CIMAR-LA/CIIMAR – Centro Interdisciplinar de Investigaçao Marinha e Ambiental, Laboratório de Ecotoxicologia, Rua dos Bragas 289, 4050-123 Porto, Portugal
Francisco Amat
Affiliation:
Instituto de Acuicultura de Torre de la Sal (CSIC), 12595 Ribera de Cabanes, Castellón, Spain
Amparo Torreblanca
Affiliation:
Departamento de Biología Funcional y Antropología Física, Facultad de Ciencias Biológicas, Universidad de Valencia, C/ Doctor Moliner s/n, Burjasot, Valencia, Spain
Lúcia Guilhermino
Affiliation:
ICBAS – Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Departamento de Estudos de Populações, Laboratório de Ecotoxicología, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal CIMAR-LA/CIIMAR – Centro Interdisciplinar de Investigaçao Marinha e Ambiental, Laboratório de Ecotoxicologia, Rua dos Bragas 289, 4050-123 Porto, Portugal
Juan C. Navarro
Affiliation:
Instituto de Acuicultura de Torre de la Sal (CSIC), 12595 Ribera de Cabanes, Castellón, Spain
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Abstract

The gilthead seabream is the most important Mediterranean aquacultured fish species. The main objective of this study was to investigate whether copper sulphate bath treatments used routinely in aquaculture have effects on important physiological functions of early life stages of the gilthead seabream (Sparus aurata). Fingerlings (80–90 days, 0.27 ± 0.06 g) were exposed to copper sulphate baths at 0, 0.25, 0.5 and 1.5 mg L−1 during 24 h. Effects on the central nervous function were evaluated analysing brain acetylcholinesterase activity (AChE). Oxidative stress was assessed by the quantification of lipid peroxidation (LP). Heat shock proteins (HSP70) were used as a general response to chemical stress, RNA/DNA ratio as an indicator of growth, and effects on detoxification function were estimated using glutathione–S-transferases activity (GST). Exposure of S. aurata fingerlings to copper sulphate induced increased lipid peroxidation. In contrast, there were no significant changes in AChE activity, HSP70 levels, RNA/DNA ratio or GST activity. Particularly, the absence of response in GST activity points towards a limited extent of the putative damage caused by the treatments at the highest doses (evidenced through the levels of peroxidation), since this enzyme is directly implicated in detoxification processes involving redox cyclic products. The results suggest that copper sulphate baths at the conditions tested are safe treatments for S. aurata fingerlings.

Keywords

Copper sulphateBiomarkersAcetylcholinesteraseLipid peroxidationGSTRNADNASeabream
Type
Research Article
Information
Aquatic Living Resources , Volume 20 , Issue 3 , July 2007 , pp. 263 - 270
Copyright
© EDP Sciences, IFREMER, IRD, 2007

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References

Antognelli, C., Romani, R., Baldracchini, F., De Santis, A., Andreani, G., Talesa, V., 2003, Different activity of glyoxalase system enzymes in specimens of Sparus auratus exposed to sublethal copper concentrations. Chem-Biol. Interact. 142, 297-305. CrossRef
Barata, C., Navarro, J.C., Varo, I., Riva, M.C., Arun, S., Porte, C., 2005, Changes in antioxidant enzyme activities, fatty acid composition and lipid peroxidation in Daphnia magna during the aging process. Comp. Biochem. Physiol. B 140, 81-90. CrossRef
Barber, I., Baird, D.J., Calow, P., 1994, Effect of cadmium and ration level on oxygen-consumption, RNA concentration and RNA-DNA Ratio in 2 clones of Daphnia-magna Straus. Aquat. Toxicol. 30, 249-258. CrossRef
Benton, M.J., Nimrod, A.C., Benson, W.H., 1994, Evaluation of growth and energy-storage as biological markers of DDT exposure in sailfin mollies. Ecotoxicol. Environ. Safe. 29, 1-12. CrossRef
Boone, A.N., Vijayan, M.M., 2002, Constitutive heat shock protein 70 (HSC70) expression in rainbow trout hepatocytes: effect of heat shock and heavy metal exposure. Comp. Biochem. Physiol. C 132, 223-233.
Bradford, M.M., 1976, Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal. Biochem. 72, 248-254. CrossRef
Carreau, N.D., Pyle, G.G., 2005, Effect of copper exposure during embryonic development on chemosensory function of juvenile fathead minnows (Pimephales promelas). Ecotoxicol Environ. Safe. 61, 1-6. CrossRef
Carvalho, C.S., Fernandes, M.N., 2006, Effect of temperature on copper toxicity and hematological responses in the neotropical fish Prochilodus scrofa at low and high pH. Aquaculture 251, 109-117. CrossRef
Chen, M.F., Apperson, J.A., Marty, G.D., Cheng, Y.W., 2006, Copper sulfate treatment decreases hatchery mortality of larval white seabass Atractoscion nobilis. Aquaculture 254, 102-114. CrossRef
Clemmesen, C., 1994, The effect of food availability, age or size on the rna/dna ratio of individually measured herring larvae - laboratory calibration. Mar. Biol. 118, 377-382. CrossRef
Dautremepuits, C., Paris-Palacios, S., Betoulle, S., Vernet, G., 2004, Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan. Comp. Biochem. Physiol. C 137, 325-333.
De Boeck, G., De Wachter, B., Vlaeminck, A., Blust, R., 2003, Effect of cortisol treatment and/or sublethal copper exposure on copper uptake and heat shoch protein levels in common carp, Cyprinus carpio. Environ. Toxicol. Chem. 22, 1122-1126. CrossRef
Del Ramo J., Torreblanca A., Díaz-Mayans J., 1993, Toxicidad de los metales. In: Mas A., Azcue J. (Eds.). Metales en sistemas biológicos. Promociones y Publicaciones Universitarias, S.A., Barcelona, pp. 143-162.
Dethloff, G.M., Bailey, H.C., Maier, K.J., 2001, Effects of dissolved copper on select hematological, biochemical, and immunological parameters of wild rainbow trout (Oncorhynchus mykiss). Arch. Environ. Contam. Toxicol. 40, 371-380.
Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M., 1961, A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7, 88-95. CrossRef
Feng, Q., Boone, A.N., Vijayan, M.M., 2003, Copper impact on heat shock protein 70 expression and apoptosis in rainbow trout hepatocytes. Comp. Biochem. Physiol. C 135, 345-355.
Frasco, M., Guilhermino, L., 2002, Effects of dimethoate and beta-naphthoflavone on selected biomarkers of Poecilia reticulata. Fish Physiol. Biochem. 26, 149–156. CrossRef
Frasco, M.F., Fournier, D., Carvalho, F., Guilhermino, L., 2005, Do metals inhibit acetylcholinesterase (AChE)? Implementation of assay conditions for the use of AChE activity as a biomarker of metal toxicity. Biomarkers 10, 360-375. CrossRef
Gabryelak, T., Klekot, J., 1985, The effect of paraquat on the peroxide metabolism enzymes in erythrocytes of fresh-water fish species. Comp. Biochem. Physiol. C 81, 415-418. CrossRef
George S.G., 1994, Enzymology and molecular biology of phase II xenobiotic-conjugating enzymes in fish. In: Malins D.C., Ostrander G.K. (Eds.), Aquatic Toxicology; Molecular, Biochemical and Cellular Perspectives, Lewis Publishers, CRC Press, pp. 37-85.
Geracitano, L.A., Monserrat, J.M., Bianchini, A., 2004, Oxidative stress in Laeonereis acuta (Polychaeta, Nereididae): environmental and seasonal effects. Mar. Environ. Res. 58, 625-630. CrossRef
Grosell, M., McDonald, M.D., Wood, C.M., Walsh, P.J., 2004a, Effects of prolonged copper exposure in the marine gulf toadfish (Opsanus beta) I. Hydromineral balance and plasma nitrogenous waste products. Aquat. Toxicol. 68, 249-262.
Grosell, M., McDonald, M.D., Walsh, P.J., Wood, C.M., 2004b, Effects of prolonged copper exposure in the marine gulf toadfish (Opsanus beta) II: copper accumulation, drinking rate and Na+/K+-ATPase activity in osmoregulatory tissues. Aquat. Toxicol. 68, 263-275. CrossRef
Guilhermino, L., Lopes, M.C., Carvalho, A.P., Soares, A.M.V.M., 1996, Inhibition of acetylcholinesterase activity as effect criterion in acute tests with juvenile Daphnia magna. Chemosphere 32, 727–738. CrossRef
Habig, W.H., Pabst, M.J., Jakoby, W.B., 1974, Glutathione S transferases. J. Biol. Chem. 249, 7130-7137.
Hansen, B.H., Romma, S., Softeland, L.I.R., Olsvik, P.A., Andersen, R.A., 2006, Induction and activity of oxidative stress-related proteins during waterborne Cu-exposure in brown trout (Salmo trutta). Chemosphere 65, 1707-1714. CrossRef
Ibiam, U., Grant, A., 2005, RNA/DNA ratios as a sublethal endpoint for large-scale toxicity tests with the nematode Caenorhabditis elegans. Environ. Toxicol. Chem. 24, 1155-1159. CrossRef
Kamunde, C., Grosell, M., Higgs, D., Wood, C.M., 2002, Copper metabolism in actively growing rainbow, trout (Oncorhynchus mykiss): interactions between dietary and waterborne copper uptake. J. Exp. Biol. 205, 279-290.
Key, P.B., Fulton, M.H., 2002, Characterization of cholinesterase activity in tissues of the grass shrimp (Palaemonetes pugio). Pestic. Biochem. Phys. 72, 186-192. CrossRef
Kim, S.G., Kang, J.C., 2004, Effect of dietary copper exposure on accumulation, growth and hematological parameters of the juvenile rockfish, Sebastes schlegeli. Mar. Environ. Res. 58, 65-82. CrossRef
Livingstone, D.R., 2001, Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar. Pollut. Bull. 42, 656-666. CrossRef
Mance G., 1987, Pollution threat of heavy metals in aquatic environments. London. Elsevier Applied Science.
Martínez-Álvarez, R.M., Morales, A.E., Sanz, A., 2005, Antioxidant defenses in fish: biotic and abiotic factors. Rev. Fish Biol. Fish. 15, 75-88. CrossRef
Miliou, H., Zaboukas, N., Moraitou-Apostolopoulou, M., 1998, Biochemical composition, growth, and survival of the guppy, Poecilia reticulata, during chronic sublethal exposure to cadmium. Arch. Environ. Contam. Toxicol. 35, 58-63. CrossRef
Monteiro, S.M., Mancera, J.M., Fontainhas-Fernandes, A., Sousa, M., 2005, Copper induced alterations of biochemical parameters in the gill and plasma of Oreochromis niloticus. Comp. Biochem. Physiol. C 141, 375-383.
Narvaez, N., Lodeiros, U., Nusetti, O., Lemus, M., Maeda-Martinez, A.N., 2005, Uptake, depuration and effect of cadmium on the green mussel Perna viridis (L. 1758) (Mollusca: Bivalvia). Cienc. Mar. 31, 91-102. CrossRef
Ohkawa, H., Ohishi, N., Yagi, K., 1979, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358. CrossRef
Paris-Palacios, S., Biagianti-Risbourg, S., Vernet, G., 2000, Biochemical and (ultra)structural hepatic perturbations of Brachydanio rerio (Teleostei, Cyprinidae) exposed to two sublethal concentrations of copper sulfate. Aquat. Toxicol. 50, 109-124. CrossRef
Peyghan, R., Razijalaly, M., Baiat, M. Rasekh, A., 2003, Study of bioaccumulation of copper in liver and muscle of common carp Cyprinus carpio after copper sulphate bath. Aquacult. Int. 11, 597-604. CrossRef
Ploch, S.A., Lee, Y.P., MacLean, E., Di Giulio, R.T., 1999, Oxidative stress in liver of brown bullhead and channel catfish following exposure to tert-butyl hydroperoxide. Aquat. Toxicol. 46, 231-240. CrossRef
Pottinger, T.G., Carrick, T.R., Yeomans, W.E., 2002, The three-spined stickleback as an environmental sentinel: effects of stressors on whole-body physiological indices. J. Fish Biol. 61, 207-229. CrossRef
Rábago-Castro, J.L., Sanchez, J.G., Perez-Castaneda, R., Gonzalez-Gonzalez, A., 2006, Effects of the prophylactic use of Romet (R)-30 and copper sulfate on growth, condition and feeding indices in Channel catfish (Ictalurus punctatus). Aquaculture 253, 343-349. CrossRef
Rigos, G., Pavlidis, M., Divanach, P., 2001, Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: a case report. Bull. Eur. Assoc. Fish Pathol. 21, 33-36.
Roméo, M., Bennani, N., Gnassia-Barelli, M., Lafaurie, M., Girard, J.P., 2000, Cadmium and copper display different responses towards oxidative stress in the kidney of the sea bass Dicentrarchus labrax. Aquat. Toxicol. 48, 185-194. CrossRef
Sanchez, W., Palluel, O., Meunier, L., Coquery, M., Porcher, J.M., Ait-Aissa, S., 2005, Copper-induced oxidative stress in three-spined stickleback: relationship with hepatic metal levels. Environ. Toxicol. Pharmacol. 19, 177-183. CrossRef
Sanders B.M., 1990, Stress proteins: potential as multitiered biomarkers. In: Shugart, L., McCarty, J. (Eds.). Environmental Biomarkers, Lewis Publishers, Chelsea, MI, pp. 165-191.
Sanders, B.M., 1993, Stress proteins in aquatic organisms - an environmental perspective. Crit. Rev. Toxicol. 23, 49-75. CrossRef
Sanders, B.M., Nguyen, J., Martin, L.S., Howe, S.R., Coventry, S., 1995, Induction and subcellular localization of two major stress proteins in response to copper in the fathead minnow Pimephales promelas. Comp. Biochem. Physiol. C 112, 335-343.
Schlotfeldt H.J., 1992, Current practices of chemotherapy in fish culture. In: Michel, C., Alderman, D.J. (Eds.). Chemotherapy in Aquaculture: from theory to reality. Office International des Epizooties, Paris, pp. 25-38.
Sellin, M.K., Tate-Boldt, E., Kolok, A.S., 2005, Acclimation to Cu in fathead minnows: Does age influence the response? Aquat. Toxicol. 74, 97-109.
Song, S.B., Xu, Y., Zhou, B.S., 2006, Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio). Chemosphere 65, 699-706. CrossRef
Stohs, S.J., Bagchi, D., 1995, Oxidative mechanisms in the toxocity of metal ions. Free Radical Biol. Med. 18, 321- 336. CrossRef
Towbin, H., Staehelin, T., Gordon, J., 1979, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets - Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354. CrossRef
Üner, N., Oruc, E.O., Sevgiler, Y., Sahin, N., Durmaz, H., Usta, D., 2006, Effects of diazinon on acetylcholinesterase activity and lipid peroxidation in the brain of Oreochromis niloticus. Environ. Toxicol. Pharmacol. 21, 241-245. CrossRef
van der Oost, R., Beyer, J., Vermeulen, N.P.E., 2003, Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ. Toxicol. Pharmacol. 13, 57-149. CrossRef
van Heerden, D., Vosloo, A., Nikinmaa, M., 2004, Effects of short-term copper exposure on gill structure, metallothionein and hypoxia-inducible factor-1 alpha (HIF-1 alpha) levels in rainbow trout (Oncorhynchus mykiss). Aquat. Toxicol. 69, 271-280. CrossRef
Varó, I., Navarro, J.C., Amat, F., Guilhermino, L., 2003, Effect of dichlorvos on cholinesterase activity of the European sea bass (Dicentrarchus labrax). Pestic. Biochem. Phys. 75, 61-72. CrossRef
Varó, I., Serrano, R., Pitarch, E., Amat, F., López, F.J., Navarro, J.C., 2002, Bioaccumulation of chlorpyrifos through an experimental food chain: study of protein HSP70 as biomarker of sublethal stress in fish. Arch. Environ. Con. Tox. 42, 229-235.
Vidal, E.A.G., DiMarco, P., Lee, P., 2006, Effects of starvation and recovery on the survival, growth and RNA/DNA ratio in loliginid squid paralarvae. Aquaculture 260, 94-105. CrossRef
Walker C.H., Hopkin S.P., Sibly R.M., Peakall D.B., 2003, Principles of ecotoxicology. Taylor& Francis, London.
Wo, K.T., Lam, P.K.S., Wu, R.S.S., 1999, A comparison of growth biomarkers for assessing sublethal effects of cadmium on a marine gastropod, Nassarius festivus. Mar. Pollut. Bull. 39, 165-173. CrossRef
Wong, P.P.K., Chu, L.M., Wong, C.K., 1999, Study of toxicity and bioaccumulation of copper in the silver sea bream Sparus sarba. Environ. Int. 25, 417-422. CrossRef
Yang, Z.P., Dettbarn, W.D., 1996, Diisopropylphosphorofluoridate-induced cholinergic hyperactivity and lipid peroxidation. Toxicol. Appl. Pharmacol. 138, 48-53. CrossRef
Yang, Z.P., Morrow, J., Wu, A.P., Roberts, L.J., Dettbarn, W.D., 1996, Diisopropylphosphorofluoridate-induced muscle hyperactivity associated with enhanced lipid peroxidation in vivo. Biochem. Pharmacol. 52, 357-361. CrossRef
Zhou, B.S., Wu, R.S.S., Randall, D.J., Lam, P.K.S., 2001, Bioenergetics and RNA/DNA ratios in the common carp (Cyprinus carpio) under hypoxia. J. Comp. Physiol. B 171, 49-57. CrossRef