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Changes of sperm morphology, volume, density and motility and seminal plasma composition in Barbus barbus(Teleostei: Cyprinidae) during the reproductive season

Published online by Cambridge University Press:  09 February 2008

Sayyed Mohammad Hadi Alavi ,
Martin Psenicka ,
Marek Rodina ,
Tomas Policar  and
Otomar Linhart
Sayyed Mohammad Hadi Alavi
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 389 25 Vodnany, Czech Republic
Martin Psenicka
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 389 25 Vodnany, Czech Republic
Marek Rodina
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 389 25 Vodnany, Czech Republic
Tomas Policar
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 389 25 Vodnany, Czech Republic
Otomar Linhart
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 389 25 Vodnany, Czech Republic
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Abstract

Eighteen spermiating males were randomly selected from a hatchery-reared stock and electronically tagged to record changes in their sperm quality parameters (spermatozoa morphology, ultrastructure and motility, ionic composition and osmolality of the seminal plasma, and sperm volume and density) during the spawning season. Stripping was performed at the beginning of March, April and May. The Barbus barbus spermatozoon has a head without acrosome, a midpiece with 4–6 mitochondria and proximal and distal centrioles, and a flagellum with the typical 9+2 pairs of microtubules. Apart from posterior width of the midpiece, morphological and ultrastructural parameters changed significantly during the reproductive season; generally by decreasing toward the end of reproductive season. Sperm volume also decreased from 0.42 in March to 0.15 ml in May, and density from 18.81 in March to 12.45 × 109 spz ml−1 in May. Osmolality (mOsmol kg−1) was 268 ± 4, 276 ± 2 and 268 ± 2 in March, April and May respectively. Chloride, sodium, calcium and potassium ion concentrations (mM) did not show significant differences between March and April (Cl−: 125.3 vs. 120.5, Na+: 75.7 vs. 69.7, Ca2+: 0.4 vs. 0.3 and K+: 84.7 vs. 84.0). The percentage of motile spermatozoa at 15 s post activation did not show a significant difference between dates, but the highest spermatozoa velocity at 15 s post activation was observed in April (91.4 ± 3.2 µm s−1) and then decreased significantly towards the end of the reproductive season (80.6 µm s−1 in May). However, lowest spermatozoa velocity was measured in March (70.4 ± 1.9 µm s−1). This study supports the hypothesis that longer spermatozoa swim faster. Within one stripping, velocity and percentage motility decreased significantly with time post activation. In conclusion, changes observed in B. barbus sperm parameters during the reproductive season, suggest there is association between such changes and spermatozoa aging processes.

Keywords

Seminal plasmaSpermMotilityMorphology of spermatozoaBarbus barbus
Type
Brief Report
Information
Aquatic Living Resources , Volume 21 , Issue 1 , January 2008 , pp. 75 - 80
Copyright
© EDP Sciences, IFREMER, IRD, 2008

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