Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-22T16:35:21.819Z Has data issue: false hasContentIssue false
  • English
  • Français

Changes in molecular genetic variation at AFLP loci associated with naturalization and domestication of the Pacific oyster (Crassostrea gigas)⋆

Published online by Cambridge University Press:  15 April 2011

Mark D. Camara
Mark D. Camara*
Affiliation:
USDA, Agricultural Research Service, Shellfish Genetics, 2030 SE Marine Science Dr., Newport, Oregon 97365, USA
*
a Corresponding author: [email protected]
Get access

Abstract

The Pacific oyster (Crassostrea gigas) is an important commercial species in the US Pacific Northwest with a history of production initially based on wild-caught seed imported directly from the Miyagi region of northern Japan (1920s–1970s) followed by an extended period of seed collection from a small number of naturalized, self-recruiting populations in US estuaries (early 1970s-present) and more recently through large-scale hatchery production of seed oysters (mid 1970s-present). I studied the genetic level consequences of each of these major transformations of the oyster industry by examining the patterns of private alleles (bands unique to one sample), the number of polymorphic loci, expected heterozygosity, genetic distance between populations and genetic divergence between individuals within and among three native Pacific oyster populations in Japan (Hiroshima, Miyagi, Midori River), five naturalized populations in North America (Pipestem Inlet BC, Nootka Inlet BC, Dabob Bay WA, Willapa Bay, WA, and Tillamook Bay, OR), two in New Zealand (Chance Bay and Kaipara Harbor), and seven domesticated and selectively bred cohorts from an ongoing genetic improvement program on the West Coast of the United States using amplified fragment length polymorphism (AFLP) markers. All but one of the naturalized populations in both the USA and New Zealand are genetically more similar to native populations from the Ariake Sea (Midori River) than to the Miyagi region of their origin, but all domesticated stocks more closely resemble the wild Miyagi population. According to local oyster producers, the one exceptional naturalized population (Tillamook) is a very recent colonization derived from farmed oysters. Such consistency is unexpected under random genetic drift, and I speculate that both natural and artificial selection may have altered AFLP allele frequencies in this species in the course of naturalization and domestication.

Type
Research Article
Information
Aquatic Living Resources , Volume 24 , Issue 1 , January 2011 , pp. 35 - 43
Copyright
© EDP Sciences, IFREMER, IRD 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

⋆

Supporting information is only available in electronic form at http://www.alr-journal.org

References

Appleyard, S.A., Ward, R.D., 2006, Genetic diversity and effective population size in mass selection lines of Pacific oyster (Crassostrea gigas). Aquaculture 254, 148–159. CrossRefGoogle Scholar
Bonin, A., Ehrich, D., Manel, S., 2007, Statistical analysis of amplified fragment length polymorphism data: a toolbox for molecular ecologists and evolutionists. Mol. Ecol. 16, 3737–3758. CrossRefGoogle ScholarPubMed
Breese W.P., Wick W.Q., 1974, Oyster farming: culturing, harvesting, and processing a product of the Pacific coast area. Extension Marine Advisory Program, Marine Science Publication SG Number 13, Newport, OR, Oregon State University, p. 8.
Cassell D.L., 2002, A Randomization-test Wrapper for SASR◯PROCs, Proceedings of the Twenty-Seventh Annual SASR◯Users Group International Conference, Cary, NC, SAS Institute Inc., http://www2.sas.com/proceedings/sugi27/p251-27.pdf.
Clark, J.E., Langmo, R., 1979, Oyster seed hatcheries on the US west coast: an overview. Mar. Fish. Rev. 41, 10–16. Google Scholar
English, L.J., Maguire, G.B., Ward, R.D., 2000, Genetic variation of wild and hatchery populations of the Pacific oyster, Crassostrea gigas (Thunberg), in Australia. Aquaculture 187, 283–298. CrossRefGoogle Scholar
Excoffier, L., Hofer, T., Foll, M., 2009, Detecting loci under selection in a hierarchically structured population. Heredity 103, 285–298. CrossRefGoogle Scholar
Feely, R.A., Sabine, C.L, Lee, K., Berelson, W., Kleypas, J., Fabry, V.J., Millero, F.J., 2004, Impact of Anthropogenic CO2 on the CaCO3 System in the Oceans. Science 305, 362–366. CrossRefGoogle Scholar
Felsenstein, J., 1989, PHYLIP – Phylogeny Inference Package (version 3.2). Cladistics 5, 164–166. Google Scholar
Felsenstein J., 1993, PHYLIP (Phylogeny Inference Package) version 3.5c, Distributed by the author. Department of Genetics, University of Washington, Seattle.
Hedgecock D., 1994, Does variance in reproductive success limit effective population sizes of marine organisms? In: Beaumont A.R. (Ed.), Genetics and Evolution of Aquatic Organisms. London, Chapman Hall, p. 122–134.
Hedgecock, D., Chow, V., Waples, R.S., 1992, Effective population numbers of shellfish brood stocks estimated from temporal variances in allelic frequencies. Aquaculture 108, 215–232. CrossRefGoogle Scholar
Hershberger, W.K., Perdue, J.A., Beattie, J.H., 1984, Genetic selection and systematic breeding in Pacific oyster culture. Aquaculture 39, 237–245. CrossRefGoogle Scholar
Jones G., Jones B., 1983, Methods for setting hatchery produced oyster larvae, Marine Resources Branch, Ministry of Environment, British Columbia, p. 61.
Langdon, C., Evans, F., Jacobson, D., Blouin, M., 2003, Yields of cultured Pacific oysters Crassostrea gigas Thunberg improved after one generation of selection. Aquaculture 220, 227–244. CrossRefGoogle Scholar
Li, L., Guo, X., 2004, AFLP-based Genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg. Mar. Biotechnol. 6, 26–36. CrossRefGoogle ScholarPubMed
Lynch, M., Milligan, B.G., 1994, Analysis of population genetic-structure with RAPD markers. Mol. Ecol. 3, 91–99. CrossRefGoogle ScholarPubMed
Newkirk, G.F., 1978, A discussion of possible sources of inbreeding in hatchery stock and associated problems. Proc. World Aquac. Soc. 9, 93–100. Google Scholar
Peakall, R., Smouse, P.E., 2006, genalex 6: genetic analysis in Excel, Population genetic software for teaching and research. Mol. Ecol. Notes 6, 288–295. CrossRefGoogle Scholar
SAS, 2004, SAS OnlineDocR◯, Version 9.1 http://support.sas.com/documentation/onlinedoc. Cary, NC, SAS Institute Inc.
Taris, N., Boudry, P., Bonhomme, F., Camara, M.D., Lapegue, S., 2009, Mitochondrial and nuclear DNA analysis of genetic heterogeneity among recruitment cohorts of the European flat oyster Ostrea edulis. Biol. Bull. 217, 233–241. CrossRefGoogle Scholar
Troost, K., 2010, Causes and effects of a highly successful marine invasion: case-study of the introduced Pacific oyster Crassostrea gigas in continental NW European estuaries. J. Sea Res. 64, 145–165. CrossRefGoogle Scholar
Vekemans, X., Beauwens, T., Lemaire, M., Roldan-Ruiz, I., 2002, Data from amplified fragment length polymorphism (AFLP) markers show indication of size homoplasy and of a relationship between degree of homoplasy and fragment size. Mol. Ecol. 11, 139–151. CrossRefGoogle Scholar
Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M., 1995, AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23, 4407–4414. CrossRefGoogle ScholarPubMed
Wang, H., Guo, X., 2008, Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR (polymerase chain reaction), J. Shellfish Res. 27, 481–487. CrossRefGoogle Scholar
Zhivotovsky, L.A., 1999, Estimating population structure in diploids with multilocus dominant DNA markers. Mol. Ecol. 8, 907–913. CrossRefGoogle ScholarPubMed
Supplementary material: PDF

OLM - alr 24(1) 2011 p. 35 - Changes in molecular genetic variation at AFLP ...

Tables S1 and S2

Download OLM - alr 24(1) 2011 p. 35 - Changes in molecular genetic variation at AFLP ...(PDF)
PDF 33.2 KB