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Use of histopathology, PCR and in situ hybridization methods to detect the parasite Mikrocytos sp. in Pacific oyster Crassostrea gigas from the northern coast of the Yellow Sea, China

Published online by Cambridge University Press:  17 March 2010

Zhongwei Wang ,
Yubo Liang  and
Xin Lu
Zhongwei Wang
Affiliation:
Environmental Science and Engineering College, Dalian Maritime University, Dalian 116026, PR China
Yubo Liang
Affiliation:
Marine Environmental Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, PR China
Xin Lu
Affiliation:
Life Science and Technology College, Dalian Fisheries University, Dalian 116023, PR China
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Abstract

Mikrocytos mackini is the etiological agent of Denman Island disease, which causes significant mortalities in commercially important bivalve species, including the Pacific oyster, Crassostrea gigas. A close relative of M. mackini, Mikrocytos sp., was recently detected in oysters imported into France from Canada. In this study, we examined Pacific oysters from the northern coast of the Yellow Sea, China. Of the one hundred samples examined histologically, a microcell parasite was found in the tissues of four oysters. To identify whether the parasite was Mikrocytos sp., DNA was extracted from the oysters and polymerase chain reaction (PCR) amplifications were performed with primers (Mikrocytos-F and Mikrocytos-R), which yielded the expected 522 bp fragment. DNA sequencing of these products confirmed that they were identical to the corresponding 18S region of Mikrocytos sp. (100%) and had close similarity to M. mackini (89%). In situ hybridization (ISH) also was performed in this study, and the primer pair MM-like (CCTGTCCTATGTCGGGCAGG) hybridized with the Pacific oyster parasite. This is the first report of Mikrocytos sp. in the Pacific oyster from the coast of China. Although this study suggests a low prevalence of the parasite in China, its potential threat to aquaculture should be considered.

Keywords

ParasiteMikrocytos mackiniMikrocytos sp.HistologyPolymerase chain reactionIn situ hybridizationCrassostrea gigas
Type
Note
Information
Aquatic Living Resources , Volume 23 , Issue 1 , January 2010 , pp. 125 - 130
Copyright
© EDP Sciences, IFREMER, IRD, 2010

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