Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-23T03:10:23.319Z Has data issue: false hasContentIssue false
  • English
  • Français

Modelling the accumulation of PSP toxins in Thau Lagoon oysters (Crassostrea gigas) from trials using mixed cultures of Alexandrium catenella and Thalassiosira weissflogii

Published online by Cambridge University Press:  17 May 2007

Patrick Lassus ,
Zouher Amzil ,
Régis Baron ,
Véronique Séchet ,
Laurent Barillé ,
Eric Abadie ,
Michèle Bardouil ,
Manoella Sibat ,
Philippe Truquet  and
Jean-Baptiste Bérard
...Show all authors
Patrick Lassus
Affiliation:
IFREMER, EMP, BP 21105, 44311 Nantes Cedex 3, France
Zouher Amzil
Affiliation:
IFREMER, EMP, BP 21105, 44311 Nantes Cedex 3, France
Régis Baron
Affiliation:
IFREMER, STAM, BP 21105, 44311 Nantes Cedex 3, France
Véronique Séchet
Affiliation:
IFREMER, EMP, BP 21105, 44311 Nantes Cedex 3, France
Laurent Barillé
Affiliation:
Université de Nantes, Laboratoire d'Ecophysiologie marine intégrée, EA 2663, Faculté des Sciences et des Techniques, BP 92208, 44322 Nantes Cedex 3, France
Eric Abadie
Affiliation:
IFREMER, LERLR, Station de Sète, avenue Jean Monnet, BP 171, 34203 Sète Cedex, France
Michèle Bardouil
Affiliation:
IFREMER, EMP, BP 21105, 44311 Nantes Cedex 3, France
Manoella Sibat
Affiliation:
IFREMER, EMP, BP 21105, 44311 Nantes Cedex 3, France
Philippe Truquet
Affiliation:
IFREMER, EMP, BP 21105, 44311 Nantes Cedex 3, France
Jean-Baptiste Bérard
Affiliation:
IFREMER, EMP, BP 21105, 44311 Nantes Cedex 3, France
Marielle Gueguen
Affiliation:
IFREMER, EMP, BP 21105, 44311 Nantes Cedex 3, France
Get access

Abstract

In October and November 2003 a bloom of the toxic dinoflagellate Alexandrium catenella was observed in the North-east zone of Thau lagoon (French Mediterranean coast). Sea water samples were collected every hours to evaluate time-related variations of phytoplankton concentrations and to compare the relative ratio of A. catenella versus other phytoplankton species during the outbreak. From these observations, trials using recirculated sea water systems were performed to: i) evaluate the physiological effects on oyster of increasing proportions of A. catenella within a mixed microalgal diet where the non-toxic diatom Thalassiosira weissflogii was present at concentration: 1500 cells ml−1, ii) compare the effect of two temperatures (12 °C and 18 °C) on paralytic toxin accumulation rates in oyster flesh by ion-pairing high performance liquid chromatography (IP-HPLC) detection, iii) analyse toxin biotransformation during the contamination process, iv) evaluate the role of the different types of oyster tissue on the bioaccumulation mechanism.

The results showed: i) a significant effect of temperature increase on clearance rate and toxin uptake, ii) no detectable time-related effects of toxic algal food on pre-ingestion sorting or on toxin profiles in oyster flesh, iii) either negative or positive effects of A. catenella concentrations on toxin uptake (“threshold” effect), iv) high amounts of toxins in the digestive gland, accounting for more than 80% of overall shellfish toxicity. The daily amount of toxins (Q tox) taken up by each oyster was evaluated by means of a global one-compartment model. However a two-compartment model finally gave the best match with real contamination kinetics, since it integrated both toxins sequestered in oyster tissues and toxins moving in through the digestive tract.

Keywords

Paralytic shellfish poisoningCrassostrea gigasModeltoxin contentAlexandrium catenellaMediterranean Sea
Type
Research Article
Information
Aquatic Living Resources , Volume 20 , Issue 1: New developments in coastal environment research: results from the national coastal environment program , January 2007 , pp. 59 - 67
Copyright
© EDP Sciences, IFREMER, IRD, 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baron, R., Couedel, M., Joret, C., Garen, P., Truquet, P., Masselin, P., Bardouil, M., Lassus, P., 2006, Continuous fluorescence recording as a way to improve Pacific oyster (Crassostrea gigas) models of paralytic shellfish toxin accumulation. Aquat. Living Resour. 19, 77-84. CrossRef
Blanco, J., Moroño, A., Franco, J., Reyero, M.I., 1997, PSP detoxification kinetics in the mussel Mytilus galloprovincialis. One- and two- compartment models and the effect of some environmental variables. Mar. Ecol. Prog. Ser. 158, 165-175.
Bougrier, S., Geairon, P., Deslous-Paoli, J.M., Bacher, C., Jonquières, G., 1995, Allometric relationships and effects of temperature on clearance and oxygen consumption rates of Crassostrea gigas (Thunberg). Aquaculture 134, 143-154. CrossRef
Bougrier, S., Lassus, P., Bardouil, M., Masselin, P., Truquet, P., 2003, PSP accumulation yields and feeding time activity in the Pacific oyster (Crassostrea gigas) and king scallop (Pecten maximus). Aquat. Living Resour. 16, 347-352. CrossRef
Bougrier S., Lassus P., Beliaeff B., Bardouil M., Masselin P., Truquet P., Matignon F. 2001, Feeding behaviour of individuals and groups of king scallops (Pecten maximus) contaminated experimentally with PSP toxins and detoxified. In: Hallegraeff G.M., Blackburn S.I., Bolch C.J., Lewis R.J. (Eds.) Harmful algal Blooms 2000, IOC-Unesco, pp. 407-410.
Bricelj, V.M., Lee, J.H., Cembella, A.D., Anderson, D.M., 1990, Uptake kinetics of paralytic shellfish toxins from the dinoflagellate Alexandrium fundyense in the mussel Mytilus edulis. Mar.Ecol. Prog. Ser. 63, 177-188. CrossRef
Bricelj V.M., Shumway S.E. 1998, Paralytic shellfish toxins in bivalve molluscs occurrence, transfer kinetics, and biotransformation. Rev. Fish. Sci. 6, 4 315-383
Cembella, A.D., Shumway, S.E., Lewis, N.I., 1993, Anatomical distribution and spatio-temporal variation in paralytic shellfish toxin composition in two bivalves species from the Gulf of Maine. J. Shellfish Res. 12, 389-403.
Cembella, A.D., Shumway, S.E., Larocque, R., 1994, Sequestering and putative biotransformation of paralytic shellfish toxins by the sea scallops Placopecten magellanicus: seasonal and spatial scales in natural populations. J. Exp. Mar. Biol. Ecol. 180, 1-22. CrossRef
Chen C.Y, Chou, H.N, 2001. Accumulation and depuration of paralytic shellfish poisoning toxins by purple clam Hiatula rostrata Lighttoot. Toxicon 39, 1029-1034.
Honsell G., Poletti R., Pompei M., Sidari L., Milandri A., Casadei C., Viviani R., 1996, Alexandrium minutum Halim. and PSP contamination in the Northern Adriatic Sea (Mediterranean sea). In: Yasumoto T., Oshima Y., Fukuyo Y. (Eds.). Harmful and Toxic Algal Blooms, IOC-Unesco, pp. 77-80.
Joutei L.T., 1998. Gymnodinium catenatum Graham blooms on Moroccan waters. In: Reguera B., Blanco J., Fernandez M.L.,Wyatt T. (Eds.). Harmful Algae, Xunta de Galicia and IOC-Unesco, pp. 66-67.
Laabir M., Génovési-Giunti B., Barré N., Vaquer A., Collos Y., Erard-Le Denn E., Cecchi P., Pons V., Bilbent B., 2004, The resting cyst of Alexandrium catenella a dinoflagellate responsible for Harmful Algal Blooms in Thau Lagoon (Western French Mediterranean coast). In: Steidinger K.A., Landsberg J.H., Tomas C.R., Vargo G.A. (Eds.) Harmful Algae 2002, Florida Fish and Wildlife Conservation Commission, Florida Institute of Oceanography and IOC-Unesco, pp. 26-28.
Lassus P., Abadie E., Amzil Z., Belin C., Comps M.A., Elzière-Papayanni P., Le Bec C., Marcaillou-Le Baut C., Nezan E., Poggi R., 1999, Contamination de l'étang de Thau par Alexandrium tamarense. Ifremer, Bilans et prospectives.
Lassus, P., Baron, R., Garen, P., Truquet, P., Masselin, P., Bardouil, M., Leguay, D., Amzil, Z., 2004, Paralytic shellfish poison outbreaks in the Penzé estuary: environmental factors affecting toxin uptake on oyster Crassotrea gigas. Aquat. Living Resour. 17, 207-214. CrossRef
Lassus P., Marcaillou-Le Baut C., 2001, Contamination, transformation et détoxication des produits marins. In: Lassus P., Frémy J.M. (Eds.) Toxines d'algues dans l'alimentation, Ifremer-AFSSA, pp. 403-446.
Lilly, E.L., Kulis, D.M., Gentien, P., Anderson, D.M., 2002, Paralytic Shellfish Poisoning toxins in France linked to a human-introduced strain of Alexandrium catenella from the western Pacific: evidence from DNA and toxin analysis. J. Plankton Res. 24, 443-452. CrossRef
Masselin P., Amzil Z., Abadie E., Carreras A., Chiantella C., Le Bec C., Nézan E., Truquet P., 2001. Paralytic shellfish poisoning on the French Mediterranean coast in autumn 1998: Alexandrium tamarense complex (Dinophyceae) as causative agent. In: Hallegraeff G.M., Blackburn S.I., Bolch C.J. Lewis R.J. (Eds.) Harmful Algal Blooms 2000, IOC-Unesco, pp. 26-29.
Moroño A., 2000, Cinética de intoxicación de toxinas PSP en Mytilus galloprovincialis Lmk., Tesis de doctorado, Universidad de Santiago de Compostela.
Moroño A., Maneiro J., Pazos Y., Blanco J., 1998, Modelling the accumulation of PSP toxins in Galician mussels: results and perspectives, In : Reguera B., Blanco J., Fernández M.L., Wyatt T. (Eds.) Harmful Algae, Xunta de Galicia and IOC-Unesco, pp. 441-444.
Navarro J.M., Muñoz M.G., Contreras A.M., 2006. Temperature as a factor regulating growth and toxin content in the dinoflagellate Alexandrium catenella. Harmful Algae 5, 762-769.
Oshima Y., 1995, Chemical and enzymatic transformation of paralytic shellfish toxins in marine organisms. In: Lassus P., Arzul G., Erard E., Gentien P. Marcaillou C. (Eds.) Harmful Algal Blooms, Lavoisier, pp. 475-480.
Samsur M., Yamaguchi Y., Sagara T., Takatani T., Arakawa O., Noguchi T., 2006. Accumulation and depuration profiles of PSP toxins in the short-necked clam Tapes japonica fed with the toxic dinoflagellate Alexandrium catenella. Toxicon 48, 323-330.
Silvert W., Bricelj M., Cembella A. 1998, Dynamic modelling of PSP toxicity in the surfclam (Spisula solidissima): multicompartmental kinetics and biotransformation. In: Reguera B., Blanco J., Fernandez M.L., Wyatt T. (Eds.) Harmful Algal Blooms. IOC-Unesco, pp. 437-440.
Vila M., Delgado M., Camp J., 2001, First detection of widespread toxic events caused by Alexandrium catenella in the Mediterranean sea. In: Hallegraeff G.M., Blackburn S.I., Bolch C.J., Lewi R.J. (Eds.) Harmful Algal Blooms 2000, IOC-Unesco, pp. 8-11.
Vila, M., Garcés, E., Masó, M., Camp, J., 2001, Is the distribution of the toxic dinoflagellate Alexandrium catenella expanding along the NW Mediterranean coast? Mar. Ecol. Prog. Ser. 222, 73-83. CrossRef
Yamamoto, T., Flynn, K.J., Takayama, H., 2003, Application of a two-compartment one-toxin model to predict the toxin accumulation in Pacific oysters in Hiroshima Bay, Japan. Fish. Sci. 69, 944-950. CrossRef