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Isolation and characterization of polymorphic microsatellite loci in black sea bream (Acanthopagrus schlegeli ) by cross-species amplification with six species of the Sparidae family

Published online by Cambridge University Press:  27 October 2007

Yun-Guo Liu ,
Ling-Xiao Liu ,
Zhen-Xing Wu ,
Hong Lin ,
Ba-Fang Li  and
Xiu-Qin Sun
Yun-Guo Liu
Affiliation:
Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China The First Institute of Oceanography, National Oceanic Administration of China, Qingdao 266061, China Agricultural Product Inspection Center, Shandong Entry-Exit Inspection & Quarantine Bureau, Qingdao 266002, China
Ling-Xiao Liu
Affiliation:
Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China Linyi Academy of Agricultural Sciences, Linyi 276012, China
Zhen-Xing Wu
Affiliation:
Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China Agricultural Product Inspection Center, Shandong Entry-Exit Inspection & Quarantine Bureau, Qingdao 266002, China
Hong Lin
Affiliation:
Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China
Ba-Fang Li
Affiliation:
Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China
Xiu-Qin Sun
Affiliation:
The First Institute of Oceanography, National Oceanic Administration of China, Qingdao 266061, China
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Abstract

We tested cross-species amplification of 68 existing microsatellite loci in 6 species of the Sparidae family: Acanthopagrus butcheri, Sparus aurata, Pagrus auratus, Chrysophrys major, Pagellus bogaraveo, Pagellus erythrinus and one species of Bothidae, Paralichthys olivaceus. Of the 68 loci screened, sixteen were found to be polymorphic when tested in 20 individual black sea bream, Acanthopagrus schlegeli. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosity ranged from 0.55 to 0.95, and from 0.58 to 0.87, respectively. Our results show that cross-species amplification of known microsatellite loci in closely related species is a highly promising source of microsatellite markers for A. schlegeli.

Keywords

MicrosatellitesCross-species amplificationAcanthopagrus schlegeli
Type
Research Article
Information
Aquatic Living Resources , Volume 20 , Issue 3 , July 2007 , pp. 257 - 262
Copyright
© EDP Sciences, IFREMER, IRD, 2007

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