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Identification of four mud crab species (genus Scylla) using ITS-1 and 16S rDNA markers

Published online by Cambridge University Press:  15 March 2004

Hideyuki Imai ,
Jin-Hua Cheng ,
Katsuyuki Hamasaki  and
Ken-Ichi Numachi
Hideyuki Imai*
Affiliation:
Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0804, Japan
Jin-Hua Cheng
Affiliation:
Tungkang Marine Laboratory, Taiwan Fisheries Research Institute, Tangkang, Pingtung, Taiwan 928, Republic of China
Katsuyuki Hamasaki
Affiliation:
Yaeyama Station, National Center for Stock Enhancement, Fisheries Research Agency (FRA) and Ishigaki Tropical Station, Seikai National Fisheries Research Institute, FRA, Ishigaki, Okinawa 907-0451, Japan
Ken-Ichi Numachi
Affiliation:
School of Marine Science and Technology, Tokai University, Shimizu, Shizuoka 424-8610, Japan
*
[email protected]
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Abstract

The first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA and mitochondrial DNA 16S rRNA were amplified by Polymerase chain reaction (PCR) using genomic DNA extracted from adult tissue of four species of Scylla spp. and the first zoeal stages of S. serrata, S. paramamosain and S. olivacea as template. Using the ITS-1 region, variation in product fragment length was found to be useful for distinguishing S. serrata and S. olivacea from two other species. The other two species (S. paramamosain and S. tranquebarica) could be identified using the restriction endonuclease Hha I. Using 16S rDNA, all four species were identified using PCR-restriction fragment length polymorphism (RFLP) by double digestion with DraI and HindIII. These genetic markers can be used for hybridization breeding studies and in field studies of larval and juvenile mud crabs of the genus Scylla.

Keywords

Larval stageSpecies identificationPCR-RFLPMud crabScylla spp.
Type
Brief Report
Information
Aquatic Living Resources , Volume 17 , Issue 1 , January 2004 , pp. 31 - 34
Copyright
© EDP Sciences, IFREMER, IRD, 2004

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