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Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla)

Published online by Cambridge University Press:  15 March 2005

Stefano Peruzzi ,
Béatrice Chatain  and
Bruno Menu
Stefano Peruzzi
Affiliation:
IFREMER, Laboratoire de Recherche Piscicole de Méditerranée, Chemin de Maguelone, 34250 Palavas-les-Flots, France Norwegian College of Fishery Science, University of Tromsø, 9037 Tromsø, Norway
Béatrice Chatain
Affiliation:
IFREMER, Laboratoire de Recherche Piscicole de Méditerranée, Chemin de Maguelone, 34250 Palavas-les-Flots, France
Bruno Menu
Affiliation:
IFREMER, Laboratoire de Recherche Piscicole de Méditerranée, Chemin de Maguelone, 34250 Palavas-les-Flots, France
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Abstract

The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (±CI) was 1.55 ± 0.02 pg for D. labrax, 1.90 ± 0.03 pg for S. aurata, 1.57 ± 0.02 pg for L. ramada, and 2.43 ± 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant differences in genome size between males and females in either D. labrax or L. ramada. / Anguilla anguilla

Keywords

DNA contentTeleostsDicentrarchus labrax / Sparus aurata / Liza ramada
Type
Research Article
Information
Aquatic Living Resources , Volume 18 , Issue 1 , January 2005 , pp. 77 - 81
Copyright
© EDP Sciences, IFREMER, IRD, 2005

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