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Evaluation of antiviral activity in hemolymph from oysters Crassostrea rhizophorae and Crassostrea gigas

Published online by Cambridge University Press:  22 June 2006

Márcia C. Carriel-Gomes ,
Jadel M. Kratz ,
Vanessa D.M. Müller ,
Célia R.M. Barardi  and
Cláudia M.O. Simões
Márcia C. Carriel-Gomes
Affiliation:
Laboratório de Virologia Aplicada, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, UFSC, Campus Universitário Trindade, Florianópolis, SC, Brazil
Jadel M. Kratz
Affiliation:
Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, UFSC, Campus Universitário Trindade, Florianópolis, SC, Brazil
Vanessa D.M. Müller
Affiliation:
Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, UFSC, Campus Universitário Trindade, Florianópolis, SC, Brazil
Célia R.M. Barardi
Affiliation:
Laboratório de Virologia Aplicada, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, UFSC, Campus Universitário Trindade, Florianópolis, SC, Brazil
Cláudia M.O. Simões
Affiliation:
Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, UFSC, Campus Universitário Trindade, Florianópolis, SC, Brazil
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Abstract

This study describes the in vitro antiviral activity of two species of oyster hemolymph (Crassostrea rhizophorae and C. gigas) collected on Southern Brazilian coastline. Either the acellular and cellular fractions were tested against herpes simplex type-1 (HSV-1), human adenovirus respiratory strain (AdV-5) and simian rotavirus (RV-SA11) by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The evaluation of viral inhibition was performed using four different strategies: simultaneous, pre-infection and post-infection treatments and virucidal assays. The cellular fraction from Crassostrea rhizophorae showed the most prominent inhibition of HSV-1 and AdV-5 replication, particularly in post-infection treatment assay. No inhibition of rotavirus replication was observed. Both oyster hemolymph fractions showed virucidal activity against all tested virus in non-cytotoxic concentrations.

Keywords

AntiviralOyster hemolymphCrassostrea rhizophoraeCrassostrea gigasBrazil
Type
Research Article
Information
Aquatic Living Resources , Volume 19 , Issue 2 , April 2006 , pp. 189 - 193
Copyright
© EDP Sciences, IFREMER, IRD, 2006

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