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Do dietary amino acid profiles affect performance of larval gilthead seabream?

Published online by Cambridge University Press:  25 July 2007

Cláudia Aragão ,
Luís E.C. Conceição ,
Marc Lacuisse ,
Manuel Yúfera  and
Maria Teresa Dinis
Cláudia Aragão
Affiliation:
CCMAR, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
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Abstract

Live preys commonly used in fish larval rearing seem to be imbalanced in terms of amino acids. Manipulation of their amino acid composition is difficult, but the use of microencapsulated diets allows this manipulation. This study analysed the effect of amino acid supplementation, in order to compensate for dietary amino acid imbalances, on growth and survival of gilthead seabream (Sparus aurata) larvae. Larvae were reared until 32 days after hatching, in a closed recirculating water system (19 °C), using only live food (rotifers and Artemia). Thereafter, larvae were fed Artemia or one of the experimental microencapsulated diets: supplemented with indispensable amino acids (IAAsup diet), or supplemented with dispensable amino acids (DAAsup diet). Experiment lasted for 10 days. Dietary supplementation with indispensable amino acids resulted in A/E ratios [(each indispensable amino acid) × (total indispensable amino acids)−1 × 1000]more similar to the ones of larval seabream and in higher IAA:DAA ratios than in the DAAsup diet. Survival was similar in larvae fed the IAAsup diet (75%) or Artemia (87%), but was significantly lower in larvae fed the DAAsup diet (52%). Larvae from all treatments more than doubled their average dry weight during the experimental period. Final dry weight was similar in larvae fed both microcapsules, but these were lighter than larvae fed with Artemia. Relative growth rate (RGR) and total biomass production tended to be higher in larvae fed the IAAsup (RGR = 9% day−1) than the DAAsup diet (RGR = 7.5% day−1) and only in this last treatment these parameters were significantly lower than in larvae fed with Artemia. Therefore, dietary supplementation with indispensable amino acids resulted in a more balanced dietary amino acid profile, which significantly increased survival. Further studies introducing microdiets earlier in the development seem necessary in order to optimise growth.

Keywords

Amino acidsGrowth, Fish LarvaeMicroencapsulated dietsSparus aurataSurvival
Type
Research Article
Information
Aquatic Living Resources , Volume 20 , Issue 2 , April 2007 , pp. 155 - 161
Copyright
© EDP Sciences, IFREMER, IRD, 2007

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References

Alam, M.S., Teshima, S., Koshio, S., Ishikawa, M., 2002, Arginine requirement of juvenile Japanese flounder Paralichthys olivaceus estimated by growth and biochemical parameters. Aquaculture 205, 127-140. CrossRef
Aragão, C, Conceição, L.E.C., Fyhn, H.J., Dinis, M.T., 2004a, Estimated amino acid requirements during early ontogeny in fish with different life styles: gilthead seabream (Sparus aurata) and Senegalese sole (Solea senegalensis). Aquaculture 242, 589-605. CrossRef
Aragão, C., Conceição, L.E.C., Dinis, M.T., Fyhn, H.J., 2004b, Amino acid pools of rotifers and Artemia under different conditions: nutritional implications for fish larvae. Aquaculture 234, 429-445. CrossRef
Aragão, C., Conceição, L.E.C., Martins, D., Rønnestad, I., Gomes, E., Dinis, M.T., 2004c, A balanced dietary amino acid profile improves amino acid retention in post-larval Senegalese sole (Solea senegalensis). Aquaculture 233, 293-304. CrossRef
Arai, S., 1981, A purified test diet for coho salmon, Oncorhynchus kisutch, fry. Bull. Jpn. Soc. Sci. Fish. 47, 547-550. CrossRef
Ballantyne J.S., 2001, Amino acid metabolism. In: Wright PA, Anderson AJ (Eds.) Nitrogen Excretion. Fish Physiology 20, Academic Press, San Diego, USA, pp. 77-107.
Cahu, C.L., Zambonino Infante, J.L., Takeuchi, T., 2003, Nutritional components affecting skeletal development in fish larvae. Aquaculture 227, 245-258. CrossRef
Carter C.G., Houlihan D.F., 2001, Protein synthesis. In: Wright PA, Anderson AJ (Eds.) Nitrogen Excretion. Fish Physiology, Vol.20, Academic Press, San Diego, USA, pp. 31-75.
Choo, P.-S., Smith, T.K., Cho, C.Y., Ferguson, H.W., 1991, Dietary excesses of leucine influence growth and body composition of rainbow trout. J. Nutr. 121, 1932-1939.
Cohen S.A., Meys M., Tarvin T.L., 1989, The Pico-Tag Method - A Manual of Advanced Techniques for Amino Acid Analysis. Waters, Bedford, USA.
Conceição, L.E.C., Grasdalen, H., Rønnestad, I., 2003a, Amino acid requirements of fish larvae and post-larvae: new tools and recent findings. Aquaculture 227, 221-232. CrossRef
Conceição, L.E.C., Grasdalen, H., Dinis, M.T., 2003b, A new method to estimate the relative bioavailability of individual amino acids in fish larvae using 13C-NMR spectroscopy. Comp. Biochem. Physiol. B 134, 103-109. CrossRef
Fernández-Díaz, C., Yúfera, M., 1997, Detecting growth in gilthead seabream, Sparus aurata L, larvae fed microcapsules. Aquaculture 153, 93-102. CrossRef
Goff, J.B., Gatli, D.M. III, 2004, Evaluation of different sulfur amino acid compounds in the diet of red drum, Sciaenops ocellatus, and sparing value of cystine for methionine. Aquaculture 241, 465-477. CrossRef
Green, J.A., Hardy, R.W., Brannon, E.L., 2002, The optimum dietary essential: nonessential amino acid ratio for rainbow trout (Oncorhynchus mykiss), which maximizes nitrogen retention and minimizes nitrogen excretion. Fish Physiol. Biochem. 27, 109-115. CrossRef
Hughes, S.G., 2003, Tests of a microencapsulated amino acid diet for larval and juvenile striped bass. World Aquac. 34, 12-15.
Kvåle, A., Nordgreen, A., Tonheim, S.K., Hamre, K., 2007, The problem of meeting dietary protein requirements in intensive aquaculture of marine fish, with emphasis on Atlantic halibut (Hippoglossuss hippoglossus L.). Aquac. Nutr. 13, 170-185. CrossRef
Kolkovski, S., Tandler, A., Izquierdo, M.S., 1997a, Effects of live food and dietary digestive enzymes on the efficiency of microdiets for seabass (Dicentarchus labrax) larvae. Aquaculture 148, 313-322. CrossRef
Kolkovski, S., Arieli, A., Tandler, A., 1997b, Visual and chemical cues stimulate microdiet ingestion in sea bream larvae. Aquac. Int. 5, 527-536. CrossRef
Mambrini, M., Kaushik, S.J., 1995, Indispensable amino acid requirements of fish: correspondence between quantitative data and amino acid profiles of tissue proteins. J. Appl. Ichthyol. 11, 240-247. CrossRef
Moretti A., Fernadez-Criado M.P., Cittolin G, Guidastri R, 1999, Manual on Hatchery Production of Seabass and Gilthead Seabream. Food and Agriculture Organization of the United Nations, Rome, Italy.
Rønnestad I., Conceição L.E.C., 2005, Aspects of protein and amino acids digestion and utilization by marine fish larvae. In: Starck JM, Wang T (Eds.) Physiological and Ecological Adaptations to Feeding in Vertebrates, Science Publishers, Enfield, New Hampshire, USA, pp. 389-416.
Takeuchi, T., Wang, Q., Furuita, H., Hirota, T., Ishida, S., Hayasawa, H., 2003, Development of microparticle diets for Japanese flounder Paralichthys olivaceus larvae. Fish. Sci. 69, 547-554. CrossRef
Tandler A., Kolkovski S., 1991, Rates of ingestion and digestibility as limiting factors in the successful use of microdiets in Sparus aurata larval rearing. In: Lavens P, Sorgeloos P, Jaspers E, Ollevier F (Eds.) Larvi'91 - Fish & Crustacean Larviculture Symposium, European Aquaculture Society, Special Publication No. 15, Gent, Belgium, pp. 169-171.
Yúfera, M., Pascual, E., Fernández-Díaz, C., 1999, A highly efficient microencapsulated food for rearing early larvae of marine fish. Aquaculture 177, 249-256. CrossRef
Yúfera, M., Fernández-Díaz, C., Pascual, E., Sarasquete, M.C., Moyano, F.J., Díaz, M., Alarcón, F.J., García-Gallego, M., Parra, G., 2000, Towards an inert diet for first-feeding gilthead seabream Sparus aurata L. larvae. Aquacult. Nutr. 6, 143-152. CrossRef
Yúfera, M., Kolkovski, S., Fernández-Díaz, C., Dabrowski, K., 2002, Free amino acid leaching from a protein-walled microencapsulated diet for fish larvae. Aquaculture 214, 273-287. CrossRef
Yúfera, M., Kolkovski, S., Fernández-Díaz, C., Rinchard, J., Lee, K.J., Dabrowski, K., 2003, Delivering bioactive compounds to fish larvae using microencapsulated diets. Aquaculture 227, 277-291. CrossRef
Watanabe, T., Kiron, V., 1994, Prospects in larval fish dietetics. Aquaculture 124, 223-251. CrossRef
Wilson R.P., 1994, Amino Acid Requirements of Finfish. In: D'Mello JPF (Ed.) Amino Acids in Farm Animal Nutrition, CAB International, Wallingford, UK, pp. 377-399.