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Calcein and manganese experiments for marking the shell of thecommon cockle (Cerastoderma edule): tidal rhythm validation of incrementsformation

Published online by Cambridge University Press:  30 September 2010

Kélig Mahé ,
Elise Bellamy ,
Franck Lartaud  and
Marc de Rafélis
Kélig Mahé*
Affiliation:
IFREMER, Centre Manche-mer du Nord, Sclerochronology Centre, Laboratoire Ressources halieutiques, 150 quai Gambetta, BP 699, 62321 Boulogne-sur-mer, France
Elise Bellamy
Affiliation:
IFREMER, Centre Manche-mer du Nord, Sclerochronology Centre, Laboratoire Ressources halieutiques, 150 quai Gambetta, BP 699, 62321 Boulogne-sur-mer, France IFREMER, Centre Manche-mer du Nord, Laboratoire Environnement côtier et Ressources aquacoles, 150 quai Gambetta, BP 699, 62321 Boulogne-sur-mer, France
Franck Lartaud
Affiliation:
UPMC Univ Paris 06, CNRS FRE 3350, Laboratoire d’Ecogéochimie des Environnements benthiques (LECOB), Observatoire océanologique, av. du Fontaulé, 66650 Banyuls-sur-mer, France
Marc de Rafélis
Affiliation:
UPMC Univ Paris 06, UMR 7193, iSTeP, Laboratoire Biominéralisations et Environnements sédimentaires, Case Postale 116, 4 place Jussieu, 75252 Paris Cedex 05, France
*
a Corresponding author:[email protected]
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Abstract

This work focuses on investigating the potential of calcein and manganese as growthmarkers of the common cockle (Cerastoderma edule) in the bay of Somme(France). Recapture of shells, previously marked using a chemical marking and then bred innatural conditions, was performed in order to determine the shell growth patterns. Calceinmarking has shown a fluorescent increment in shells after only 30 min immersion time at150 mg L-1, but also for shells immersed 3 h at 50 mg L-1. Likewise,manganese shell marking was revealed under cathodoluminescence for shells immersed 1 h at120 mg L-1 as well as for shells which spent 4 h at 90 mg L-1. Anumerical analysis performed on each marked cockles has revealed 23 micro-incrementsbetween the mark and the ventral edge of the valves, corresponding to the 23 tides thatoccurred during the 12 days at liberty post marking. The periodicity of incrementformation is thus validated for a tidal frequency. The growth rates of C.edule, ranged from 11.67 to 19.94 μm d-1, decreasedsignificantly with increasing shell length. This preliminary study gives a clue to theunderstanding of cockle growth and could be used in shellfish production for cockle agemonitoring, but also for chemical analysis to learn more about biomineralization processof this species.

Keywords

CalceinManganeseMarkingCathodoluminescenceShellfishCerastoderma edule
Type
Research Article
Information
Aquatic Living Resources , Volume 23 , Issue 3 , July 2010 , pp. 239 - 245
Copyright
© EDP Sciences, IFREMER, IRD 2010

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