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Isolation and spectral characterization of cadmium binding metallothionein

Published online by Cambridge University Press:  16 November 2007

Hyun Park ,
In-Young Ahn ,
Heeseon J. Choi  and
Jung Youn Ji
Hyun Park*
Affiliation:
Korea Polar Research Institute, Korea Ocean Research and Development Institute, Songdo-dong 7-50, Yeonsu-gu, Incheon 406-840, South Korea
In-Young Ahn
Affiliation:
Korea Polar Research Institute, Korea Ocean Research and Development Institute, Songdo-dong 7-50, Yeonsu-gu, Incheon 406-840, South Korea
Heeseon J. Choi
Affiliation:
Korea Polar Research Institute, Korea Ocean Research and Development Institute, Songdo-dong 7-50, Yeonsu-gu, Incheon 406-840, South Korea Present address:Geosystem Research Corporation, 306 Hanlim Human Tower, 1-40 Geumjung-Dong, Gunpo 435-824, South Korea
Jung Youn Ji
Affiliation:
Korea Polar Research Institute, Korea Ocean Research and Development Institute, Songdo-dong 7-50, Yeonsu-gu, Incheon 406-840, South Korea
*
*Corresponding author:[email protected]
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Abstract

A cadmium (Cd)-binding protein was isolated and characterized from the Antarctic clam Laternula elliptica after experimental exposure to a high concentration of Cd. Cd-binding metallothioneins (MTs) in the cytosol were purified using a procedure based on gel permeation and ion-exchange chromatography. The purified MTs were recognized by MT antibodies in a Western blotting assay. MALDI-TOF MS analyses showed that the molecular mass of the purified MTs was 7.27 kDa, which is typical of MTs found in marine invertebrates. The Cd binding to MT, reflected by the redistribution of Cd ions, was monitored by spectrophotometry. The absorption spectra profiles indicated the presence of Cd-MT complexes, and a 4 nm red shift of the unresolved lowest energy-absorption band occurred when five equivalents of Cd (II) were incorporated.

Keywords

AntarcticbivalvesCd-thiolate complexLaternulaMTspurification
Type
Biological Sciences
Information
Antarctic Science , Volume 20 , Issue 1 , February 2008 , pp. 33 - 37
Copyright
Copyright © Antarctic Science Ltd 2008

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