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Diversity of Lecidea (Lecideaceae, Ascomycota) species revealed by molecular data and morphological characters

Published online by Cambridge University Press:  02 December 2010

Ulrike Ruprecht ,
H. Thorsten Lumbsch ,
Georg Brunauer ,
T.G. Allan Green  and
Roman Türk
Ulrike Ruprecht*
Affiliation:
Department of Organismic Biology, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria
H. Thorsten Lumbsch
Affiliation:
Department of Botany, The Field Museum, 1400 S, Lake Shore Drive, Chicago, IL 60605, USA
Georg Brunauer
Affiliation:
Department of Organismic Biology, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria
T.G. Allan Green
Affiliation:
Biological Sciences, Waikato University, Hamilton, New Zealand Vegetal II, Farmacia Facultad, Universidad Complutense, Madrid, Spain
Roman Türk
Affiliation:
Department of Organismic Biology, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria
*
[email protected]
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Abstract

The diversity of lichens, especially crustose species, in continental Antarctica is still poorly known. To overcome difficulties with the morphology based species delimitations in these groups, we employed molecular data (nuclear ITS and mitochondrial SSU rDNA sequences) to test species boundaries within the genus Lecidea. Sampling was done along a north–south transect at five different areas in the Ross Sea region (Cape Hallett, Botany Bay to Mount Suess, Taylor Valley, Darwin Area and Mount Kyffin). A total of 153 specimens were collected from 13 localities. Phylogenetic analyses also include specimens from other regions in Antarctica and non-Antarctic areas. Maximum parsimony, maximum likelihood and Bayesian analyses agreed in placing the samples from continental Antarctica into four major groups. Based on this phylogenetic estimate, we restudied the micromorphology and secondary chemistry of these four clades to evaluate the use of these characters as phylogenetic discriminators. These clades are identified as the following species Lecidea cancriformis, L. andersonii as well as the new species L. polypycnidophora Ruprecht & Türk sp. nov. and another previously unnamed clade of uncertain status, referred to as Lecidea sp. (L. UCR1).

Keywords

chemical raceslichensphylogenetic analysissecondary metabolites
Type
Research Article
Information
Antarctic Science , Volume 22 , Special Issue 6: The Latitudinal Gradient Project (LGP) , December 2010 , pp. 727 - 741
Copyright
Copyright © Antarctic Science Ltd 2010

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References

Andreev, M.P. 1990. Lichenes oasis Bangerii (Antarctis orientalis). Novitates Systematicae Plantarum non Vascularium, 27, 85–93.Google Scholar
Arguello, A., Del Prado, R., Cubas, P. Crespo, A. 2007. Parmelina quercina (Parmeliaceae, Lecanorales) includes four phylogenetically supported morphospecies. Biological Journal of the Linnean Society, 91, 455–467.CrossRefGoogle Scholar
Arnold, A.E. Lutzoni, F. 2007. Diversity and host range of foliar fungal endophytes: are tropical leaves biodiversity hotspots? Ecology, 88, 541–549.CrossRefGoogle ScholarPubMed
Broady, P.A. Weinstein, R.N. 1998. Algae, lichens and fungi in La Gorce Mountains, Antarctica. Antarctic Science, 10, 376–385.CrossRefGoogle Scholar
Buschbom, J. Barker, D. 2006. Evolutionary history of vegetative reproduction in Porpidia s.l. (lichen-forming Ascomycota). Systematic Biology, 55, 471–484.CrossRefGoogle ScholarPubMed
Castello, M. 2003. Lichens of Terra Nova Bay area, northern Victoria Land (continental Antarctica). Studia Geobotanica, 22, 3–54.Google Scholar
Culberson, C.F. Johnson, A. 1982. Substitution of methyl tertiary buthyl ether for diethyl ether in the standardized thin-layer chromatographic method for lichen products. Journal of Chromatography, 238, 483–487.CrossRefGoogle Scholar
Dodge, C.W. 1973. Lichen flora of Antarctic continent and adjacent islands. Canaan, NH: Phoenix Publishing, 399 pp.Google Scholar
Doran, P.T., Priscu, J.C., Lyons, W.B., Walsch, J.E., Fountain, A.G., McKnight, D.M., Moorhead, D.L., Virginia, R.A., Wall, D.H., Clow, G.D., Fritsen, C.H., McKay, C.P. Parsons, A.N. 2002. Antarctic climate cooling and terrestrial ecosystem response. Nature, 415, 517–520.CrossRefGoogle ScholarPubMed
Dyer, P.S. Murtagh, G.J. 2001. Variation in the ribosomal ITS-sequence of the lichens Buellia frigida and Xanthoria elegans from the Vestfold Hills, eastern Antarctica. Lichenologist, 33, 151–159.CrossRefGoogle Scholar
Filson, R.B. 1974. Studies in Antarctic lichens. II: Lichens from the Windmill Islands, Wilkes Land. Muelleria, 3, 9–36.CrossRefGoogle Scholar
Gardes, M. Bruns, T.D. 1993. ITS primers with enhanced specifity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology, 2, 113–118.CrossRefGoogle Scholar
Green, T.G.A., Schroeter, B. Sancho, L.G. 2007. Plant life in Antarctica. In Pugnaire, F.I. & Valladares, F., eds. Handbook of functional plant ecology, 2nd ed. Boca Raton, FL: CRC Press, 389–433.CrossRefGoogle Scholar
Green, T.G.A., Seppelt, R.D. Schwarz, A.-M.J. 1992. Epilithic lichens on the floor of the Taylor Valley, Ross Dependency, Antarctica. Lichenologist, 24, 57–61.Google Scholar
Green, T.G.A., Maseyk, K., Pannewitz, S. Schroeter, B. 2000. Extreme elevated in situ carbon dioxide levels around the moss Bryum subrotundifolium Jaeg., Ber. S. Gall. in Antarctica. Bibliotheca Lichenologica, 75, 397–403.Google Scholar
Grube, M. Kroken, S. 2000. Molecular approaches and the concept of species and species complexes in lichenized fungi. Mycological Research, 104, 1284–1294.CrossRefGoogle Scholar
Gunn, B.M. Walcott, R.I. 1962. The geology of the Mt. Markham region, Ross Dependency, Antarctica. New Zealand Journal of Geology and Geophysics, 5, 407–426.CrossRefGoogle Scholar
Gunn, B.M. Warren, G. 1962. Geology of Victoria Land between Mawson and Mulock glaciers, Antarctica. New Zealand Geological Survey Bulletin, No. 71, 175 pp.Google Scholar
Hale, M.E. 1987. Epilithic lichens in the Beacon Sandstone Formation, Victoria Land, Antarctica. The Lichenologist, 19, 269–287.CrossRefGoogle Scholar
Hawksworth, D.L. Rossman, A.Y. 1997. Where are all the undescribed fungi? Phytopathology, 87, 888–891.CrossRefGoogle ScholarPubMed
Hertel, H. 1987. Progress and problems in taxonomy of Antarctic saxicolous lecideoid lichens. Bibliotheca Lichenologica, 25, 219–242.Google Scholar
Hertel, H. 1988. Problems in monographing in Antarctic crustose lichens. Polarforschung, 58, 65–76.Google Scholar
Hertel, H. 2007. Notes on and records of Southern Hemisphere lecideoid lichens. Bibliotheca Lichenologica, 95, 267–296.Google Scholar
Hertel, H. 2009. A new key to cryptothalline species of the genus Lecidea (Lecanorales). Bibliotheca Lichenologica, 99, 185–204.Google Scholar
Huelsenbeck, J.P. Ronquist, F. 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.CrossRefGoogle ScholarPubMed
Inoue, M. 1991. Lecideoid lichens of Prince Olav Coast and Sôya Coast, Enderby Land, East Antarctica. Antarctic Record, 35, 217–284.Google Scholar
Inoue, M. 1995. The lichen flora and habitats of the Syowa region, continental Antarctic. The Lichenologist, 27, 451–462.CrossRefGoogle Scholar
Jacobsen, P. Kappen, L. 1988. Lichens from the Admiralty Bay region, King Gorge Island (South Shetland Islands, Antarctica). Nova Hedwigia, 46, 503–510.Google Scholar
Kappen, L., Meyer, M. Bölter, M. 1990. Ecological and physiological investigations in continental Antarctic Cryptogams. Flora, 184, 209–220.CrossRefGoogle Scholar
Kroken, S. Taylor, J.W. 2001. A gene genealogical approach to recognize phylogentic species boundaries in the lichenized fungus Letharia. Mycologia, 93, 38–53.CrossRefGoogle Scholar
Lee, J.S., Lee, H.K., Hur, J.S., Andreev, M. Hong, S.G. 2008. Diversity of the lichenized fungi in King George Island, Antarctica, revealed by phylogenetic analysis of partial large subunit rDNA sequences. Journal of Microbiology and Biotechnology, 18, 1016–1023.Google Scholar
Lücking, R., del Prado, R., Lumbsch, H.T., Will-Wolf, S., Aptroot, A., Sipman, H.J.M., Umaña, L. Chaves, J.L. 2008. Phylogenetic patterns of morphological and chemical characters and reproductive mode in the Heterodermia obscurata group in Costa Rica. Systematics and Biodiversity, 6, 31–41.CrossRefGoogle Scholar
McGonigal, D. Woodworth, L. 2001. Antarctica - the complete story. New Zealand: Global Book Publishing, 608 pp.Google Scholar
Maddison, D.R. Maddison, W.P. 2003. MacClade 4: analysis of phylogeny and character evolution. Sunderland, MA: Sinauer Associates.Google Scholar
Melick, D.R., Hovenden, M.J. Seppelt, R.D. 1994. Phytogeography of bryophyte and lichen vegetation in the Windmill Islands, Wilkes Land, continental Antarctica. Vegetatio, 111, 71–87.CrossRefGoogle Scholar
Molina, M.D.C., Crespo, A., Blanco, O., Lumbsch, H.T. Hawksworth, D.L. 2004. Phylogenetic relationships and species concepts in Parmelia s. str. (Parmeliaceae) inferred from nuclear ITS rDNA and beta-tubulin sequences. Lichenologist, 36, 37–54.CrossRefGoogle Scholar
Nylander, J.A.A., Wilgenbusch, J.C., Warren, D.L. Swofford, D.L. 2007. AWTY (Are We There Yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics, 24, 581–583.CrossRefGoogle Scholar
Ohmura, Y. Kanda, H. 2004. Taxonomic status of section Neuropogon in the genus Usnea elucidated by morphological comparisons and ITS rDNA sequences. Lichenologist, 36, 217–225.CrossRefGoogle Scholar
Ott, S., Brinkmann, M., Wirtz, N. Lumbsch, H.T. 2004. Mitochondrial and nuclear ribosomal DNA data do not support the separation of the Antarctic lichens Umbilicaria kappenii and Umbilicaria antarctica as distinct species. Lichenologist, 36, 227–234.CrossRefGoogle Scholar
Øvstedal, D.O. Lewis Smith, R.I. 2001. Lichens of Antarctica and South Georgia: a guide to their identification and ecology. Cambridge: Cambridge University Press, 411 pp.Google Scholar
Pannewitz, S., Green, T.G.A., Maysek, K., Schlensog, M., Seppelt, R., Sancho, L.G., Türk, R. Schröter, B. 2005. Photosynthetic responses of three common mosses from continental Antarctica. Antarctic Science, 17, 341–352.CrossRefGoogle Scholar
Pickard, J. Seppelt, R.D. 1984. Phytogeography of Antarctica. Journal of Biogeography, 11, 83–102.CrossRefGoogle Scholar
Posada, D. Crandall, K.A. 1998. Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.CrossRefGoogle ScholarPubMed
Seppelt, R.D., Green, T.G.A. Schroeter, B. 1995. Lichens and mosses from the Kar Plateau, southern Victoria Land, Antarctica. New Zealand Journal of Botany, 33, 203–220.CrossRefGoogle Scholar
Seppelt, R.D., Nimis, P.L. Castello, M. 1998. The genus Sarcogyne (Acarosporaceae) in Antarctica. The Lichenologist, 30, 249–258.CrossRefGoogle Scholar
Seppelt, R.D., Broady, P.A., Pickard, J. Adamson, D.A. 1988. Plants and landscape in the Vestfold Hills, Antarctica. Hydrobiologia, 165, 185–196.CrossRefGoogle Scholar
Seymour, F.A., Crittenden, P.D., Wirtz, N., Ovstedal, D.O., Dyer, P.S. Lumbsch, H.T. 2007. Phylogenetic and morphological analysis of Antarctic lichen forming Usnea species in the group Neuropogon. Antarctic Science, 19, 71–82.CrossRefGoogle Scholar
Simpson, A.L. Cooper, A.F. 2002. Geochemistry of the Darwin Glacier region granitoids, southern Victoria Land. Antarctic Science, 14, 425–426.CrossRefGoogle Scholar
Sipman, H.J.M. Aptroot, A. 2001. Where are the missing lichens? Mycological Research, 105, 1433–1439.CrossRefGoogle Scholar
Stocker-Wörgötter, E. Elix, J.A. 2002. Secondary chemistry of cultured mycobionts: formations of a complete chemosyndrome by the lichen fungus of Lobaria spathulata. The Lichenologist, 34, 351–359.CrossRefGoogle Scholar
Swofford, D.L. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). Sunderland, MA: Sinauer Associates.Google Scholar
Taylor, G. 1913. The western journeys. In Huxley, L., ed. Scott’s last expedition. London: Smith Elder, 182–291.Google Scholar
Upreti, D.K. 1996. Lecideoid lichens from the Schirmacher Oasis, East Antarctica. Willdenowia, 25, 681–686.Google Scholar
White, T.J., Bruns, T., Lee, S. Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. In Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J., eds. PCR protocols: a guide to methods and applications. San Diego, CA: Acadamic Press, 315–322.Google Scholar
Wirtz, N., Printzen, C. Lumbsch, H.T. 2008. The delimitation of Antarctic and bipolar species of Usnea, Neuropogon (Ascomycota, Lecanorales): a cohesion approach of species recognition for the Usnea perpusilla complex. Mycological Research, 112, 472–484.CrossRefGoogle ScholarPubMed
Wirtz, N., Printzen, C., Sancho, L.G. Lumbsch, H.T. 2006. The phylogeny and classification of Neuropogon and Usnea (Parmeliaceae, Ascomycota) revisited. Taxon, 55, 367–376.CrossRefGoogle Scholar
Zoller, S., Scheidegger, C. Sperisen, C. 1999. PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. The Lichenologist, 31, 511–516.CrossRefGoogle Scholar