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Cyanobacterial diversity for an anthropogenic impact assessment in the Sør Rondane Mountains area, Antarctica

Published online by Cambridge University Press:  19 December 2011

Rafael Fernández-Carazo ,
Zorigto Namsaraev ,
Marie-Jose Mano ,
Damien Ertz  and
Annick Wilmotte
Rafael Fernández-Carazo*
Affiliation:
Centre for Protein Engineering, Institute of Chemistry B6, University of Liège, 4000 Liège, Belgium
Zorigto Namsaraev
Affiliation:
Centre for Protein Engineering, Institute of Chemistry B6, University of Liège, 4000 Liège, Belgium Winogradsky Institute of Microbiology, Russian Academy of Sciences, 117312 Moscow, Russia
Marie-Jose Mano
Affiliation:
Centre for Protein Engineering, Institute of Chemistry B6, University of Liège, 4000 Liège, Belgium
Damien Ertz
Affiliation:
Départment Bryophyta-Thallophyta, National Botanical Garden of Belgium, Domain of Bouchout, 1860 Meise, Belgium
Annick Wilmotte
Affiliation:
Centre for Protein Engineering, Institute of Chemistry B6, University of Liège, 4000 Liège, Belgium
*
[email protected]
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Abstract

The recently inaugurated Belgian Princess Elisabeth Station has been built in the Sør Rondane Mountains (Dronning Maud Land). The construction site is situated close to the Utsteinen Nunatak (71°57′S, 23°20′E), on a granite ridge. Prior to the survey and construction activities, the site had been subject to limited or no direct human impacts. Therefore, a biological inventory has been started for future evaluations of human impacts in the area. This study presents the cyanobacterial diversity of ten samples from the Utsteinen ridge, the Utsteinen Nunatak in the vicinity of the station's site and the more distant Ketelersbreen Dry Valley. All samples were taken before the station was built and were studied by light microscopy and Denaturing Gradient Gel Electrophoresis. Two strains were also isolated. Two different phenol-based extraction methods, with and without detergent-based steps, were tested to improve the quality of molecular detection from these environmental samples. We observed high cyanobacterial diversity (ten morphotypes and 13 operational taxonomic units) in comparison to other Antarctic terrestrial locations and a widespread distribution pattern within the Sør Rondane Mountains area. This might reflect the habitat similarities and/or the easy local dispersal capabilities of microorganisms within the region. The high diversity and proportion of potential endemic (46%) operational taxonomic units, of which 23% were newly described, suggest that this area could have acted as a biological refuge during past glaciations.

Keywords

AntarcticbiodiversityDronning Maud LandPrincess Elisabeth StationUtsteinen Nunatak16S rRNA
Type
Biological Sciences
Information
Antarctic Science , Volume 24 , Issue 3 , 24 May 2012 , pp. 229 - 242
Copyright
Copyright © Antarctic Science Ltd 2012

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References

Azeredo, J.Oliveira, R. 1996. A new method for precipitating bacterial exopolysaccharides. Biotechnology Techniques, 10, 341–344.CrossRefGoogle Scholar
BelSPO (Belgian Federal Science Policy Office). 2007. Construction and operation of the new Belgian research station, Dronning Maud Land, Antarctica. Final Comprehensive Environmental Evaluation (CEE). 123 pp. www.belspo.be/belspo/BePoles/doc/final_CEE_en.pdf.Google Scholar
Boutte, C., Grubisic, S., Balthasart, P.Wilmotte, A. 2006. Testing of primers for the study of cyanobacterial molecular diversity by DGGE. Journal of Microbiological Methods, 65, 542–550.CrossRefGoogle Scholar
Brinkmann, M., Pearce, D.A., Convey, P.Ott, S. 2007. The cyanobacterial community of polygon soils at an inland Antarctic nunatak. Polar Biology, 30, 1505–1511.CrossRefGoogle Scholar
Büdel, B., Bendix, J., Bicker, F.R.Green, T.G.A. 2008. Dewfall as a water source frequently activates the endolithic cyanobacterial communities in the granites of Taylor Valley, Antarctica. Journal of Phycology, 44, 1415–1424.CrossRefGoogle ScholarPubMed
Convey, P., Gibson, J.A.E., Hillenbrand, C.D., Hodgson, D.A., Pugh, P.J.A., Smellie, J.L.Stevens, M.I. 2008. Antarctic terrestrial life - challenging the history of the frozen continent? Biological Reviews, 83, 103–117.CrossRefGoogle ScholarPubMed
Davey, M.C.Rothery, P. 1993. Primary colonization by microalgae in relation to spatial variation in edaphic factors on Antarctic fellfield soils. Journal of Ecology, 81, 335–343.CrossRefGoogle Scholar
Frenot, Y., Chown, S.L., Whinam, J., Selkirk, P.M., Convey, P., Skotnicki, M.Bergstrom, D.M. 2005. Biological invasions in the Antarctic: extent, impacts and implications. Biological Reviews, 80, 45–72.CrossRefGoogle ScholarPubMed
Gordon, D.A., Priscu, J.Giovannoni, S. 2000. Origin and phylogeny of microbes living in permanent Antarctic lake ice. Microbial Ecology, 39, 197–202.Google ScholarPubMed
Jukes, T.H.Cantor, C.R. 1969. Evolution of protein molecules. In Munro, H.N., ed. Mammalian protein metabolism. New York: Academic Press, 21–132.CrossRefGoogle Scholar
Hodgson, D.A., Convey, P., Verleyen, E., Vyverman, W., McInnes, S.J., Sands, C.J., Fernández-Carazo, R., Wilmotte, A., De Wever, A., Peeters, K., Tavernier, I.Willems, A. 2010. The limnology and biology of the Dufek Massif, Transantarctic Mountains 82° South. Polar Science, 4, 197–214.CrossRefGoogle Scholar
Kepner, R.L., Wharton, R.A., Collier, R.D., Cockell, C.S.Jeffrey, W.H. 2000. UV radiation and potential biological effects beneath the perennial ice cover of an Antarctic lake. Hydrobiologia, 427, 155–165.CrossRefGoogle Scholar
Kisand, V.Wikner, J. 2003. Limited resolution of 16S rDNA DGGE caused by melting properties and closely related DNA sequences. Journal of Microbiological Methods, 54, 183–191.CrossRefGoogle ScholarPubMed
Komárek, J.Anagnostidis, K. 2005. Cyanoprokaryota. 2. Oscillatoriales. In Büdel, B., Krienitz, L., Gärtner, G., Schagerl, M.,eds. Süsswasserflora von Mitteleuropa, Bd 19/2. Heidelberg: Springer, 759 pp.Google Scholar
Komárek, J., Elster, J.Komárek, O. 2008. Diversity of the cyanobacterial microflora of the northern part of James Ross Island, NW Weddell Sea, Antarctica. Polar Biology, 31, 853–865.CrossRefGoogle Scholar
Lawver, L.A.Gahagan, L.M. 2003. Evolution of Cenozoic seaways in the circum-Antarctic region. Palaeogeography, Palaeoclimatology, Palaeoecology, 198, 11–37.CrossRefGoogle Scholar
Maidak, B.L., Cole, J.R., Lilburn, T.G., Parker, C.T. Jr, Saxman, P.R., Farris, R.J., Garrity, G.M., Olsen, G.J., Schmidt, T.M.Tiedje, J.M. 2001. The RDP-II (Ribosomal Database Project). Nucleic Acids Research, 29, 173–174.CrossRefGoogle ScholarPubMed
Marshall, D.J.Coetzee, L. 2000. Historical biogeography and ecology of a continental Antarctic mite genus, Maudheimia (Acari, Oribatida): evidence for a Gondwanan origin and Pliocene-Pleistocene speciation. Zoological Journal of the Linnean Society, 129, 111–128.CrossRefGoogle Scholar
Mataloni, G., Tell, G.Wynn-Williams, D.D. 2000. Structure and diversity of soil algal communities from Cierva Point (Antarctic Peninsula). Polar Biology, 23, 205–211.CrossRefGoogle Scholar
Namsaraev, Z., Mano, M.J., Fernandez, R.Wilmotte, A. 2010. Biogeography of terrestrial cyanobacteria from Antarctic ice-free areas. Annals of Glaciology, 51, 171–177.CrossRefGoogle Scholar
Nikolausz, M., Sipos, R., Revesz, S., Szekely, A.Marialigeti, K. 2005. Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels. FEMS Microbiology Letters, 244, 385–390.CrossRefGoogle ScholarPubMed
Nübel, U., Garcia-Pichel, F.Muyzer, G. 1997. PCR primers to amplify 16S rRNA genes from cyanobacteria. Applied and Environmental Microbiology, 63, 3327–3332.CrossRefGoogle ScholarPubMed
Pattyn, F., Matsuoka, K.Berte, J. 2000. Glacio-meteorological conditions in the vicinity of the Belgian Princess Elisabeth Station, Antarctica. Antarctic Science, 22, 79–85.CrossRefGoogle Scholar
Rippka, R.J., Waterbury, J.B.Stanier, R.Y. 1981. Isolation and purification of cyanobacteria: some general principles. In Starr, M.P., Stolp, H., Trüper, H.G., Balows, A. & Schlegel, H.G., eds. The prokaryotes, vol. 1. Berlin: Springer, 212–220.CrossRefGoogle Scholar
Saitou, N.Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.Google Scholar
Schloss, P.D.Handelsman, J. 2005. Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species-richness. Applied and Environmental Microbiology, 71, 1501–1506.CrossRefGoogle ScholarPubMed
Smith, J.J., Tow, L.A., Stafford, W., Cary, C.Cowan, D.A. 2006. Bacterial diversity in three different Antarctic cold desert mineral soils. Microbial Ecology, 51, 413–421.CrossRefGoogle ScholarPubMed
Speksnijder, A., Kowalchuk, G.A., De Jong, S., Kline, E., Stephen, J.R.Laanbroek, H.J. 2001. Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences. Applied and Environmental Microbiology, 67, 469–472.CrossRefGoogle ScholarPubMed
Taton, A., Grubisic, S., Brambilla, E., De Wit, R.Wilmotte, A. 2003. Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a morphological and molecular approach. Applied and Environmental Microbiology, 69, 5157–5169.CrossRefGoogle ScholarPubMed
Taton, A., Grubisic, S., Balthasart, P., Hodgson, D.A., Laybourn-Parry, J.Wilmotte, A. 2006a. Biogeographical distribution and ecological ranges of benthic cyanobacteria in East Antarctic lakes. FEMS Microbiology Ecology, 57, 272–289.CrossRefGoogle ScholarPubMed
Taton, A., Grubisic, S., Ertz, D., Hodgson, D.A., Piccardi, R., Biondi, N., Tredici, M.R., Mainini, M., Losi, D., Marinelli, F.Wilmotte, A. 2006b. Polyphasic study of Antarctic cyanobacterial strains. Journal of Phycology, 42, 1257–1270.CrossRefGoogle Scholar
Tin, T., Fleming, Z.L., Hughes, K.A., Ainley, D.G., Convey, P., Moreno, C.A., Pfeiffer, S., Scott, J.Snape, I. 2009. Impacts of local human activities on the Antarctic environment. Antarctic Science, 21, 3–33.CrossRefGoogle Scholar
Tripati, A., Backman, J., Elderfield, H.Ferretti, P. 2005. Eocene bipolar glaciation associated with global carbon cycle changes. Nature, 436, 341–346.CrossRefGoogle ScholarPubMed
Van de Peer, Y.De Wachter, R. 1997. Construction of evolutionary distance trees with TREECON for Windows: accounting for variation in nucleotide substitution rate among sites. Computer Applications in the Biosciences, 13, 227–230.Google ScholarPubMed
Vincent, W.F. 2000. Evolutionary origins of Antarctic microbiota: invasion, selection and endemism. Antarctic Science, 12, 374–385.CrossRefGoogle Scholar
Yergeau, E., Newsham, K.K., Pearce, D.A.Kowalchuk, G.A. 2007. Patterns of bacterial diversity across a range of Antarctic terrestrial habitats. Environmental Microbiology, 9, 2670–2682.CrossRefGoogle ScholarPubMed
Wand, U.Hermichen, W.D. 2005. Late Quaternary ice level changes in central Dronning Maud Land, East Antarctica, as inferred from 14C ages of mumiyo deposits in snow petrel colonies. Geologisches Jahrbuch, B97, 237–254.Google Scholar
Wilson, K. 1992. Preparation of genomic DNA from bacteria. In Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K.,eds. Current protocols in molecular biology. New York: John Wiley & Sons, 2-10–2-12.Google Scholar
Wu, X., Zarka, A.Boussiba, S. 2000. A simplified protocol for preparing DNA from filamentous cyanobacteria. Plant Molecular Biology Reporter, 18, 385–392.CrossRefGoogle Scholar
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