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Bound water freezing in Antarctic Umbilicaria aprina from Schirmacher Oasis

Published online by Cambridge University Press:  30 March 2012

H. Harańczyk ,
P. Nowak ,
M. Bacior ,
M. Lisowska ,
M. Marzec ,
M. Florek  and
M.A. Olech
H. Harańczyk*
Affiliation:
Institute of Physics, Jagiellonian University, ul. Reymonta 4, 30-059 Cracow, Poland
P. Nowak
Affiliation:
Institute of Physics, Jagiellonian University, ul. Reymonta 4, 30-059 Cracow, Poland
M. Bacior
Affiliation:
Institute of Physics, Jagiellonian University, ul. Reymonta 4, 30-059 Cracow, Poland
M. Lisowska
Affiliation:
Institute of Botany, Jagiellonian University, Kopernika 27, 31-501 Cracow, Poland
M. Marzec
Affiliation:
Institute of Physics, Jagiellonian University, ul. Reymonta 4, 30-059 Cracow, Poland
M. Florek
Affiliation:
Institute of Physics, Jagiellonian University, ul. Reymonta 4, 30-059 Cracow, Poland
M.A. Olech
Affiliation:
Institute of Botany, Jagiellonian University, Kopernika 27, 31-501 Cracow, Poland
*
[email protected]
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Abstract

The effect of low temperature on Umbilicaria aprina collected from Schirmacher Oasis, East Antarctica, was determined over a wide range of hydration using proton free induction decays, proton nuclear magnetic resonance (NMR) spectra and differential scanning calorimetry methods. The proton NMR line is a superposition of the broad component from the solid matrix of the thallus and a narrower component from the averaged bound water pool. Proton free induction decays may be resolved into three components: a solid component well described by the Abragam function and two exponentially decaying components from water loosely bound and water tightly bound in the thallus. With decreased temperature the loosely bound water pool (freezing water) is transferred to the tightly bound water pool (non-freezing water), and vanishes below -40°C. Bound water freezing and melting temperatures decrease with the decrease of hydration level, suggesting that heterogeneous ice nucleation is responsible for water freezing. The onset of bound water freezing temperature is c. 10°C lower than the melting temperature. The U. aprina thalli do not reveal the ability to stimulated ice nucleation at higher temperature. Freeze-thaw cycles showed that for n > 5 cycles no substantial change occurs in the difference between melting and freezing temperatures.

Keywords

DSCfoliose lichenized fungifreezing resistancelichensNMR
Type
Biological Sciences
Information
Antarctic Science , Volume 24 , Issue 4 , 17 July 2012 , pp. 342 - 352
Copyright
Copyright © Antarctic Science Ltd 2012

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