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Regional variability in eukaryotic protist communities in the Amundsen Sea

Published online by Cambridge University Press:  16 April 2013

Christian Wolf ,
Stephan Frickenhaus ,
Estelle S. Kilias ,
Ilka Peeken  and
Katja Metfies
Christian Wolf*
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
Stephan Frickenhaus
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
Estelle S. Kilias
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
Ilka Peeken
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany MARUM - Centre for Marine Environmental Sciences, University of Bremen, Leobener Straße, 28359 Bremen, Germany
Katja Metfies
Affiliation:
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
*
[email protected]
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Abstract

We determined the composition and structure of late summer eukaryotic protist assemblages along a west–east transect in the Amundsen Sea. We used state-of-the-art molecular approaches, such as automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing, combined with pigment measurements via high performance liquid chromatography (HPLC) to study the protist assemblage. We found characteristic offshore and inshore communities. In general, total chlorophyll a and microeukaryotic contribution were higher in inshore samples. Diatoms were the dominant group across the entire area, of which Eucampia sp. and Pseudo-nitzschia sp. were dominant inshore and Chaetoceros sp. was dominant offshore. At the most eastern station, the assemblage was dominated by Phaeocystis sp. Under the ice, ciliates showed their highest and haptophytes their lowest abundance. This study delivers a taxon detailed overview of the eukaryotic protist composition in the Amundsen Sea during the summer 2010.

Keywords

ARISAHPLCmicrobial diversitynext-generation-sequencingphytoplankton
Type
Biological Sciences
Information
Antarctic Science , Volume 25 , Issue 6 , December 2013 , pp. 741 - 751
Copyright
Copyright © Antarctic Science Ltd 2013 

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