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Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

Published online by Cambridge University Press:  20 September 2021

Paulo E.A.S. Câmara Open the ORCID record for Paulo E.A.S. Câmara [Opens in a new window] ,
Láuren M.D. De Souza Open the ORCID record for Láuren M.D. De Souza [Opens in a new window] ,
Otávio Henrique Bezerra Pinto Open the ORCID record for Otávio Henrique Bezerra Pinto [Opens in a new window] ,
Peter Convey Open the ORCID record for Peter Convey [Opens in a new window] ,
Eduardo T. Amorim Open the ORCID record for Eduardo T. Amorim [Opens in a new window] ,
Micheline Carvalho-Silva Open the ORCID record for Micheline Carvalho-Silva [Opens in a new window]  and
Luiz Henrique Rosa Open the ORCID record for Luiz Henrique Rosa [Opens in a new window]
Paulo E.A.S. Câmara*
Affiliation:
Departamento de Botânica, Universidade de Brasília, Brasília, 70910-900, Brazil
Láuren M.D. De Souza
Affiliation:
Departamento de Microbiologia, Universidade Federal de Minas Gerais, 31270-901, Brazil
Otávio Henrique Bezerra Pinto
Affiliation:
Departamento deBiologia Celular, Universidade de Brasília, Brasília, 70910-900, Brazil
Peter Convey
Affiliation:
British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 0ET, UK
Eduardo T. Amorim
Affiliation:
Centro Nacional de Conservação da Flora/Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (CNCFlora/JBRJ), Rio de Janeiro, 22.460-030, Brazil
Micheline Carvalho-Silva
Affiliation:
Departamento de Botânica, Universidade de Brasília, Brasília, 70910-900, Brazil
Luiz Henrique Rosa
Affiliation:
Departamento de Microbiologia, Universidade Federal de Minas Gerais, 31270-901, Brazil
*
[email protected]
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Abstract

Antarctic lakes have generally simple periphyton communities when compared with those of lower latitudes. To date, assessment of microbial diversity in Antarctica has relied heavily on traditional direct observation and cultivation methods. In this study, sterilized cotton baits were left submerged for two years in two lakes on King George Island and Deception Island, South Shetland Islands (Maritime Antarctic), followed by assessment of diversity by metabarcoding using high-throughput sequencing. DNA sequences of 44 taxa belonging to four kingdoms and seven phyla were found. Thirty-six taxa were detected in Hennequin Lake on King George Island and 20 taxa were detected in Soto Lake on Deception Island. However, no significant difference in species composition was detected between the two assemblages (Shannon index). Our data suggest that metabarcoding provides a suitable method for the assessment of periphyton biodiversity in oligotrophic Antarctic lakes.

Keywords

Deception Islandhigh-throughput sequencinginternal transcribed spacerKing George Island
Type
Biological Sciences
Information
Antarctic Science , Volume 33 , Issue 6 , December 2021 , pp. 596 - 604
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Copyright
Copyright © Antarctic Science Ltd 2021

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