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Microbial responses to carbon and nitrogen supplementation in an Antarctic dry valley soil

Published online by Cambridge University Press:  19 October 2012

P.G. Dennis ,
A.D. Sparrow ,
E.G. Gregorich ,
P.M. Novis ,
B. Elberling ,
L.G. Greenfield  and
D.W. Hopkins
P.G. Dennis
Affiliation:
School of Biological and Environmental Sciences, University of Stirling, Stirling FK9 4LA, Scotland UK Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland UK
A.D. Sparrow
Affiliation:
CSIRO Sustainable Ecosystems, PO Box 2111, Alice Springs NT 0871, Australia
E.G. Gregorich
Affiliation:
Agriculture and Agri-Food Canada, Central Experimental Farm, Ottawa, K1A 0C6, Canada
P.M. Novis
Affiliation:
Manaaki Whenua - Landcare Research, PO Box 40, Lincoln 7640, New Zealand
B. Elberling
Affiliation:
Institute of Geography and Geology, University of Copenhagen, Øster Voldgade 10, DK-1350, Copenhagen K., Denmark The University Centre in Svalbard, Longyearbyen, Norway
L.G. Greenfield
Affiliation:
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8020, New Zealand
D.W. Hopkins*
Affiliation:
School of Life Sciences, Heriot-Watt University, Edinburgh, EH14 4AS, Scotland UK
*
*corresponding author: [email protected]
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Abstract

The soils of the McMurdo Dry Valleys are exposed to extremely dry and cold conditions. Nevertheless, they contain active biological communities that contribute to the biogeochemical processes. We have used ester-linked fatty acid (ELFA) analysis to investigate the effects of additions of carbon and nitrogen in glucose and ammonium chloride, respectively, on the soil microbial community in a field experiment lasting three years in the Garwood Valley. In the control treatment, the total ELFA concentration was small by comparison with temperate soils, but very large when expressed relative to the soil organic carbon concentration, indicating efficient conversion of soil organic carbon into microbial biomass and rapid turnover of soil organic carbon. The ELFA concentrations increased significantly in response to carbon additions, indicating that carbon supply was the main constraint to microbial activity. The large ELFA concentrations relative to soil organic carbon and the increases in ELFA response to organic carbon addition are both interpreted as evidence for the soil microbial community containing organisms with efficient scavenging mechanisms for carbon. The diversity of the ELFA profiles declined in response to organic carbon addition, suggesting the responses were driven by a portion of the community increasing in dominance whilst others declined.

Keywords

ester-linked fatty acidsexperimentationGarwood ValleyMcMurdo Dry Valleysmicro-organismssubstrate response
Type
Biological Sciences
Information
Antarctic Science , Volume 25 , Issue 1 , February 2013 , pp. 55 - 61
Copyright
Copyright © Antarctic Science Ltd 2012

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References

Ball, B.A., Virginia, R.A., Barrett, J.E., Parsons, A.N.Wall, D.H. 2009. Interactions between physical and biotic factors influence CO2 flux in Antarctic Dry Valley soils. Soil Biology and Biochemistry, 41, 1510–1517.CrossRefGoogle Scholar
Barrett, J.E., Virginia, R.A.Wall, D.H. 2002. Trends in resin and KCl-extractable soil nitrogen across landscape gradients in Taylor Valley, Antarctica. Ecosystems, 5, 289–299.CrossRefGoogle Scholar
Barrett, J.E., Virginia, R.A., Parsons, A.N.Wall, D.H. 2005. Potential soil organic matter turnover in Taylor Valley, Antarctica. Arctic, Antarctic, and Alpine Research, 37, 108–117.CrossRefGoogle Scholar
Barrett, J.E., Virginia, R.A., Wall, D.H.Adams, B.J. 2008. Decline in a dominant invertebrate species contributes to altered carbon cycling in a low-diversity soil ecosystem. Global Change Biology, 14, 1734–1744.CrossRefGoogle Scholar
Barrett, J.E., Virginia, R.A., Hopkins, D.W., Aislabie, J., Bargagli, R., Bockheim, J.G., Campbell, I.R., Lyons, W.B., Moorhead, D.L., Nkem, J., Sletten, R.S., Steltzer, H., Wall, D.H.Wallenstein, M. 2006. Terrestrial ecosystem processes of Victoria Land, Antarctica. Soil Biology and Biochemistry, 38, 3019–3034.CrossRefGoogle Scholar
Bölter, M., Kandeler, E., Pietr, S.J.Seppelt, R.D. 2002. Heterotrophic microbes, microbial and enzymatic activity in Antarctic soils. In Beyer, L. & Bölter, M., eds. Geoecology of Antarctic ice-free coastal landscapes. Ecological studies, vol. 154. Berlin: Springer, 189–214.CrossRefGoogle Scholar
Brady, N.C.Weil, R.R. 1999. The nature and properties of soils, 12th ed. Upper Saddle River, NJ: Prentice Hall, 881 pp.Google Scholar
Burkins, M.B., Virginia, R.A.Wall, D.H. 2002. Organic carbon cycling in Taylor Valley, Antarctica: quantifying soil reservoirs and soil respiration. Global Change Biology, 7, 113–125.CrossRefGoogle Scholar
Burkins, M.B., Virginia, R.A., Chamberlain, C.P.Wall, D.H. 2000. Origin and distribution of soil organic matter in Taylor Valley, Antarctica. Ecology, 81, 2377–2391.CrossRefGoogle Scholar
Cary, S.C., McDonald, I.R., Barrett, J.E.Cowan, D.A. 2010. On the rocks: the microbiology of Dry Valley soils. Nature Reviews Microbiology, 8, 129–138.CrossRefGoogle ScholarPubMed
Dennis, P.G., Newsham, K.K., Rushton, S.P., Ord, V.J., O'Donnell, A.G.Hopkins, D.W. 2012. Warming constrains bacterial community responses to nutrient inputs in a southern, but not northern, Maritime Antarctic soil. Soil Biology & Biochemistry, 10.1016/j.soilbio.2012.07.009.Google Scholar
Drijber, R.A., Doran, J.W., Parkhurst, A.M.Lyon, D.J. 2000. Changes in soil microbial community structure with tillage under from past sewage sludge applications. Soil Biology and Biochemistry, 32, 1419–1430.CrossRefGoogle Scholar
Elberling, B., Gregorich, E.G., Hopkins, D.W., Sparrow, A.D., Novis, P.Greenfield, L.G. 2006. Distribution and dynamics of soil organic matter in an Antarctic dry valley. Soil Biology and Biochemistry, 38, 3095–3106.CrossRefGoogle Scholar
Friedmann, E.I. 1982. Endolithic micro-organisms in the Antarctic cold desert. Science, 215, 1045–1053.CrossRefGoogle Scholar
Gregorich, E.G., Hopkins, D.W., Elberling, B., Novis, P., Greenfield, L.G., Sparrow, A.D.Rochette, P. 2006. Biogenic gas emission along a lake shore in an Antarctic dry valley. Soil Biology & Biochemistry, 38, 3120–3129.CrossRefGoogle Scholar
Gregory, A.S., Watts, C.W., Whalley, W.R., Kuan, H.L., Griffiths, B.S., Hallett, P.D.Whitmore, A.P. 2007. Physical resilience of soil to field compaction and the interactions with plant growth and microbial community structure. European Journal of Soil Science, 58, 1221–1232.CrossRefGoogle Scholar
Hinojosa, M.B., Carreira, J.A., Garcia-Ruız, R.Dick, R.P. 2005. Microbial response to heavy metal-polluted soils: community analysis from phospholipids-linked fatty acids and ester-linked fatty acids extracts. Journal of Environmental Quality, 34, 1789–1800.CrossRefGoogle ScholarPubMed
Hopkins, D.W., Waite, I.S.O'Donnell, A.G. 2011. Microbial biomass, organic matter decay and nitrogen in soils from long-term experimental grassland plots (Palace Leas meadow hay plots, UK). European Journal of Soil Science, 62, 95–104.CrossRefGoogle Scholar
Hopkins, D.W., Sparrow, A.D., Gregorich, E.G., Novis, P., Elberling, B.Greenfield, L.G. 2008b. Redistributed lacustrine detritus as a spatial subsidy of biological resources for soils in an Antarctic dry valley. Geoderma, 144, 86–92.CrossRefGoogle Scholar
Hopkins, D.W., Sparrow, A.D., Novis, P.M., Gregorich, E.G., Elberling, B.Greenfield, L.G. 2006b. Controls on the distribution of productivity and organic resources in Antarctic dry valley soils. Proceeding of the Royal Society of London, B273, 2687–2695.Google Scholar
Hopkins, D.W., Sparrow, A.D., Elberling, B., Gregorich, E.G., Novis, P., Greenfield, L.G.Tilston, E.L. 2006a. Carbon, nitrogen and temperature controls on microbial activity in soils from an Antarctic dry valley. Soil Biology and Biochemistry, 38, 3130–3140.CrossRefGoogle Scholar
Hopkins, D.W., Sparrow, A.D., Shillam, L.L., English, L.C., Dennis, P.G., Novis, P.M., Elberling, B., Gregorich, E.G.Greenfield, L.G. 2008a. Enzymatic activities in Antarctic dry valley soil: responses to carbon and nitrogen supplementation. Soil Biology and Biochemistry, 40, 2130–2136.CrossRefGoogle Scholar
Hopkins, D.W., Sparrow, A.D., Gregorich, E.G., Elberling, B., Novis, P., Fraser, F., Scrimgeour, C., Dennis, P.G., Meier-Augenstein, W.Greenfield, L.G. 2009. Isotopic evidence for the provenance and turnover of organic carbon in Antarctic dry valley soils. Environmental Microbiology, 11, 597–608.CrossRefGoogle Scholar
Killham, K. 1994. Soil ecology. Cambridge: Cambridge University Press, 260 pp.CrossRefGoogle Scholar
Leckie, S.E., Prescott, C.E., Grayston, S.J., Neufeld, J.D.Mohn, W.W. 2004. Characterization of humus microbial communities in adjacent forest types that differ in nitrogen availability. Microbial Ecology, 48, 29–40.CrossRefGoogle ScholarPubMed
Parsons, A.N., Barrett, J.E., Wall, D.H.Virginia, R.A. 2004. Soil carbon dioxide flux in Antarctic dry valley ecosystems. Ecosystems, 7, 286–295.CrossRefGoogle Scholar
Pietr, S.J., Tatur, A.Myrcha, A. 1983. Mineralization of penguin excrements in the Admiralty Bay region (King George Island, South Shetland, Antarctica). Polar Research, 4, 97–112.Google Scholar
Schutter, M.E.Dick, R.P. 2000. Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities. Soil Science Society of America Journal, 64, 1659–1668.CrossRefGoogle Scholar
Shannon, C.E. 1948. A mathematical theory of communication. The Bell System Technical Journal, 27, 379–423.CrossRefGoogle Scholar
Simmons, B.L., Wall, D.H., Adams, B.J., Ayres, E., Barrett, J.E.Virginia, R.A. 2009. Long-term experimental warming reduces soil nematode populations in the McMurdo Dry Valleys, Antarctica. Soil Biology and Biochemistry, 41, 2052–2060.CrossRefGoogle Scholar
Simpson, E.H. 1949. Measurement of diversity. Nature, 163, 688.CrossRefGoogle Scholar
Smith, J.L., Barrett, J.E., Tusnady, G., Rejtoe, L.Cary, S.C. 2010. Resolving environmental drivers of microbial community structure in Antarctic soils. Antarctic Science, 22, 673–680.CrossRefGoogle Scholar
Sparrow, A.D., Gregorich, E.G., Hopkins, D.W., Novis, P., Elberling, B.Greenfield, L.G. 2011. Resource limitations on the activity of a soil microbial community in the dry valleys of southern Victoria Land, Antarctica. Soil Science Society of America Journal, 75, 2188–2197.CrossRefGoogle Scholar
Steger, K., Jarvis, A., Smårs, S.Sundh, I. 2003. Comparison of signature lipid methods to determine microbial community structure in compost. Journal of Microbiological Methods, 55, 371–382.CrossRefGoogle ScholarPubMed
Stevens, M.I.Hogg, I.D. 2002. Expanded distributional records of Collembola and Acari in southern Victoria Land, Antarctica. Pedobiologia, 46, 485–495.CrossRefGoogle Scholar
Treonis, A.M., Wall, D.H.Virginia, R.A. 1999. Invertebrate biodiversity in Antarctic dry valley soils and sediments. Ecosystems, 2, 482–492.CrossRefGoogle Scholar
Tscherko, D., Bölter, M., Beyer, L., Chen, J., Elster, E., Kandeler, E., Kuhn, D.Blume, H-P. 2003. Biomass and enzyme activity of soil transects at King George Island, Maritime Antarctica. Arctic, Antarctic, and Alpine Research, 35, 34–47.CrossRefGoogle Scholar
Zeglin, L.H., Sinsabaugh, R.L., Barrett, J.E., Gooseff, M.N.Takacs-Vesbach, C.D. 2009. Landscape distribution of microbial activity in the McMurdo Dry Valleys: linked biotic processes, hydrology, and geochemistry in a cold desert ecosystem. Ecosystems, 12, 562–573.CrossRefGoogle Scholar
Zelles, L., Bai, Q.Y., Rackwitz, R., Chadwick, D.Beese, F. 1995. Determination of phospholipid- and lipopolysaccharide-derived fatty acids as an estimate of microbial biomass and community structures in soils. Biology and Fertility of Soils, 19, 115–123.CrossRefGoogle Scholar