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Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater

Published online by Cambridge University Press:  18 March 2019

Consolazione Caruso ,
Carmen Rizzo ,
Santina Mangano ,
Annarita Poli ,
Paola Di Donato ,
Barbara Nicolaus ,
Ilaria Finore ,
Gaetano Di Marco ,
Luigi Michaud  and
Angelina Lo Giudice Open the ORCID record for Angelina Lo Giudice [Opens in a new window]
Consolazione Caruso
Affiliation:
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Messina, Italy
Carmen Rizzo
Affiliation:
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Messina, Italy
Santina Mangano
Affiliation:
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Messina, Italy
Annarita Poli
Affiliation:
Institute of Biomolecular Chemistry, National Research Council (ICB-CNR), Pozzuoli (NA), Italy
Paola Di Donato
Affiliation:
Institute of Biomolecular Chemistry, National Research Council (ICB-CNR), Pozzuoli (NA), Italy Department of Science and Technology, University of Naples Parthenope – Centro Direzionale, Isola C4, 80143 Napoli, Italy
Barbara Nicolaus
Affiliation:
Institute of Biomolecular Chemistry, National Research Council (ICB-CNR), Pozzuoli (NA), Italy
Ilaria Finore
Affiliation:
Institute of Biomolecular Chemistry, National Research Council (ICB-CNR), Pozzuoli (NA), Italy
Gaetano Di Marco
Affiliation:
Institute for Chemical-Physical Processes, National Research Council (IPCF-CNR), Messina, Italy
Luigi Michaud
Affiliation:
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Messina, Italy
Angelina Lo Giudice*
Affiliation:
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Messina, Italy Institute for the Biological Resources and Marine Biotechnologies National Research Council (IRBIM-CNR), Messina, Italy
*
*[email protected]
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Abstract

Marinobacter sp. W1-16 from Antarctic surface seawater was analysed for the production of extracellular polymeric substances (EPSs). Enhancement of the EPS biosynthesis was carried out by evaluating the influences of the carbon source (type and concentration), temperature, pH and salinity. EPS yields varied strongly depending on sugar substrate and temperature, while pH and salinity did not strongly affect levels of EPS production. Marinobacter sp. W1-16 produced the highest quantity of EPSs when growing at 15°C and pH 8, in the presence of 2% glucose and 3% NaCl. The EPS chemical characterization revealed a molecular weight of about 260 kDa. Colorimetric assays determined a higher quantity of carbohydrate than of proteins and uronic acids, as well as the presence of sulphate, in the extracted EPSs. The monosaccharidic composition resulted in Glc:Man:Gal:GalN:GalA:GlcA in relative molar proportions of 1:0.9:0.2:0.1:0.1:0.01. Some biotechnological potentialities (i.e. emulsifying and cryoprotective actions, and heavy metal binding properties) of the EPSs were proved, suggesting possible industrial and bioremediation applications.

Keywords

bioprospectingcryoprotectionemulsifying activityEPSheavy metals
Type
Biological Sciences
Information
Antarctic Science , Volume 31 , Issue 2 , April 2019 , pp. 69 - 79
Copyright
Copyright © Antarctic Science Ltd 2019 

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Posthumous

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