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Isolation and characterization of Antarctic psychrotroph Streptomyces sp. strain INACH3013

Published online by Cambridge University Press:  13 July 2016

Paris Leonardo Lavin Open the ORCID record for Paris Leonardo Lavin [Opens in a new window] ,
Sheau Ting Yong ,
Clemente M.V.L. Wong  and
Mario De Stefano
Paris Leonardo Lavin*
Affiliation:
Laboratorio de Biorrecursos, Instituto Antártico Chileno, Punta Arenas, Chile
Sheau Ting Yong
Affiliation:
Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Sabah, Malaysia
Clemente M.V.L. Wong
Affiliation:
Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Sabah, Malaysia National Antarctic Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia
Mario De Stefano
Affiliation:
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, II University of Naples, Italy
*
[email protected]
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Abstract

An actinobacterial strain with antimicrobial activity, INACH3013, was isolated from soil collected from Antarctica. The taxonomic status of the isolate was established using a polyphasic approach. The strain was identified as belonging to the genus Streptomyces based on the scanning electron microscopic observation and partial 16S rRNA gene sequence analysis. The sequence analysis revealed that strain INACH3013 is closely related to Streptomyces fildesensis (99.8%), S. beijiangensis (98.1%) and S. purpureus (97.2%). A phylogenetic tree constructed using the partial 16S rRNA gene sequences of strain INACH3013 and closely related strains revealed that INACH3013 fell into the same subclade as S. fildesensis and S. purpureus. Strain INACH3013 was observed to be psychrotolerant, slightly halotolerant (up to 5% NaCl) and capable of inhibiting the growth of seven Gram-negative and eight Gram-positive foodborne pathogens. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of the strain is KJ624755.

Keywords

antimicrobialfoodborne pathogenspsychrotolerantSouth Shetland Islands
Type
Biological Sciences
Information
Antarctic Science , Volume 28 , Issue 6 , December 2016 , pp. 433 - 442
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Copyright
© Antarctic Science Ltd 2016 

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