Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-18T14:14:15.443Z Has data issue: false hasContentIssue false
  • English
  • Français

Antioxidant responses of in vitro shoots of Deschampsia antarctica to Polyethylene glycol treatment

Published online by Cambridge University Press:  22 January 2010

Pablo Zamora ,
Susana Rasmussen ,
Ariel Pardo ,
Humberto Prieto  and
Gustavo E. Zúñiga
Pablo Zamora
Affiliation:
Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Universidad de Santiago de Chile, Santiago de Chile, 9170022, Chile
Susana Rasmussen
Affiliation:
Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Universidad de Santiago de Chile, Santiago de Chile, 9170022, Chile
Ariel Pardo
Affiliation:
Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Universidad de Santiago de Chile, Santiago de Chile, 9170022, Chile PhD Programme in Biotechnology, Universidad de Santiago de Chile
Humberto Prieto
Affiliation:
Instituto de Investigaciones Agropecuarias (INIA), La Platina, Research Center, Santiago de Chile, Chile
Gustavo E. Zúñiga*
Affiliation:
Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Universidad de Santiago de Chile, Santiago de Chile, 9170022, Chile
*
*corresponding author: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

To understand the adaptability to environmental stresses by Deschampsia antarctica, one of the two vascular plants growing in Antarctica, we analysed the activity of several antioxidant enzymes, including peroxidase (POD, EC 1.11.1.7), ascorbate peroxidase (APX, EC 1.11.1.11), catalase (CAT, EC 1.11.1.6)) and glutathion reductase (GR, EC 1.6.4.2), in shoots subjected to drought stress (PEG-8000, -0.3 MPa). Additionally, levels of total phenolic compounds, flavonoids and ascorbate, were determined. The content of malondialdehyde (MDA), chlorophyll and hydrogen peroxide did not change as a result of PEG-8000 treatment. In addition, treated plants showed higher enzymatic activity of CAT, POD and GR in shoots than control plants. In addition, a high capacity to scavenge free radicals was also detected in stressed plants. These results seem to indicate that in D. antarctica tolerance of drought stress is associated with enhanced activity of antioxidant enzymes and free radical scavenging capacity.

Keywords

Antarctic hairgrassgramineaeosmotic stressPEG-8000reactive oxygen species (ROS)
Type
Biological Sciences
Information
Antarctic Science , Volume 22 , Issue 2 , April 2010 , pp. 163 - 169
Copyright
Copyright © Antarctic Science Ltd 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alberdi, M., Bravo, L., Gutiérrez, A., Gidekel, M.Corcuera, L. 2002. Ecophysiology of Antarctic vascular plants. Physilogia Plantarum, 115, 479–486.CrossRefGoogle ScholarPubMed
Asada, K. 1999. The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 601–639.CrossRefGoogle Scholar
Asami, D., Hong, Y., Barrett, D.Mitchell, A. 2003. Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried conventional, organic, and sustainable agricultural practices. Journal of Agricultural and Food Chemistry, 51, 1237–1241.CrossRefGoogle ScholarPubMed
Baisak, R., Rana, D., Acharya, P.B.B.Kar, M. 1994. Alteration in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress. Plant Cell Physiology, 35, 489–495.Google Scholar
Brand-Williams, W., Cuvelier, M.E.Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und Technologie, 28, 25–30.CrossRefGoogle Scholar
Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.CrossRefGoogle ScholarPubMed
Chen, T.Murata, N. 2002. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and others compatible solutes. Plant Biology, 5, 250–257.Google Scholar
Crowe, J., Crowe, L., Leslie, S.Fisk, E. 1993. Plant responses to cellular dehydration during environmental stress. mechanisms of stabilization of dry biomolecules in anhydrobiotic organism. Current Topics in Plant Physiology, 10, 11–20.Google Scholar
Ederli, L., Pasqualini, S., Batini, P.Antonielli, M. 1997. Photoinhibition and oxidative stress: effects on xanthophyll cycle, scavenger enzymes and abscisic acid content in tobacco plants. Journal of Plant Physiology, 151, 422–428.CrossRefGoogle Scholar
Fadzilla, N.M., Finch, R.P.Burdon, R.H. 1997. Salinity, oxidative stress and antioxidant responses in shoot cultures of rice. Journal of Experimental Botany, 48, 325–331.CrossRefGoogle Scholar
Foyer, C.H., Descourvieres, P.Kunert, K.J. 1994. Protein against oxygen radicals: an important defense mechanism studied in transgenic plants. Plant, Cell & Environment, 17, 507–523.CrossRefGoogle Scholar
Gamble, P.E.Burke, J.J. 1984. Effect of water stress on the chloroplast antioxidant system. 1. Alterations in glutathione reductase activity. Plant Physiology, 76, 615–621.CrossRefGoogle Scholar
Hodges, M., Delong, J., Forney, C.Prange, R. 1999. Improving the thiobarbituric acid-reactive-substance assay for estimating lipid peroxidation in plant tissue containing anthocyanin and other interfering compounds. Planta, 207, 604–611.CrossRefGoogle Scholar
Holmstrom, K.O., Somersalo, S., Mandal, A., Palva, T.Welin, B. 2000. Improved tolerance to salinity and low temperature in transgenic tobacco producing glycine betaine. Journal of Experimental Botany, 51, 177–185.CrossRefGoogle ScholarPubMed
Jain, M., Mathur, G., Koul, G.S.Sarin, N.B. 2001. Ameliorative effects of proline on salt stress induced lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). Plant Cell Reproduction, 20, 463–468.CrossRefGoogle Scholar
Jiang, M.Zhang, J. 2002. Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings. Planta, 215, 1022–1030.CrossRefGoogle ScholarPubMed
Kerk, N.M.Feldman, L.J. 1995. A biochemical model for the initiation and maintenance of the quiescent center: implications for organization of root meristems. Development, 121, 2825–2833.CrossRefGoogle Scholar
Lichtenthaler, H.Wellburn, A. 1990. Determinations of total carotenoids and chlorophylls α and β of leaf extracts in different solvents. Biochemical Society Transactions, 11, 591–592.CrossRefGoogle Scholar
Liu, M., Li, X.Q., Weber, C., Lee, C.Y., Brown, J.Liu, R.H. 2002. Antioxidant and antiproliferative activities of raspberries. Journal of Agricultural and Food Chemistry, 50, 2926–2930.CrossRefGoogle ScholarPubMed
McCue, K.F.Hanson, A.D. 1990. Drought and salt tolerance: towards understanding and application. Trends in Biotechnology, 8, 358–362.CrossRefGoogle Scholar
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plants Science, 7, 405–410.CrossRefGoogle ScholarPubMed
Murashige, T.Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–479.CrossRefGoogle Scholar
Noctor, G.Foyer, C.H. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 249–279.CrossRefGoogle ScholarPubMed
Pastori, G.M.Trippi, V.S. 1993. Antioxidative protection in a drought resistant maize strains during leaf senescence. Physilogia Plantarum, 87, 227–231.CrossRefGoogle Scholar
Pietta, P. 2000. Flavonoids as antioxidant. Journal of Natural Products, 63, 1035–1042.CrossRefGoogle Scholar
Pinhero, R., Rao, M., Paliyath, G., Murr, D.Fletcher, R. 1997. Changes in activities of antioxidant enzymes and their relationship to genetic and paclobutrazol-induced chilling tolerance of maize seedling. Plant Physiology, 114, 695–704.CrossRefGoogle Scholar
Sairam, R.K., Shukla, D.S.Saxena, D.C. 1998. Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes. Biologia Plantarum, 40, 357–364.Google Scholar
Sánchez-Fernández, R., Fricker, M., Corben, L.B., White, N.S., Sheard, N., Leaver, C.J., van Montagu, M., Inzé, D.May, M.J. 1997. Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control. Proceedings of the National Academy of Science of the United States, 94, 2745–2750.CrossRefGoogle ScholarPubMed
Schaedle, M.Bassham, J.A. 1977. Chloroplast glutathione reductase. Plant Physiology, 59, 1011–1012.CrossRefGoogle ScholarPubMed
Smirnoff, N. 1993. The role of active oxygen in response of plants to water deficit and desiccation. New Phytologist, 125, 27–58.CrossRefGoogle ScholarPubMed
Wang, W., Binocur, B.Altman, A. 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta, 218, 1–14.CrossRefGoogle ScholarPubMed
Yeo, A. 1998. Molecular biology of salt tolerance in the context of whole-plant physiology. Journal of Experimental Botany, 49, 915–929.Google Scholar
Zhao, S.Blumwald, E. 1998. Changes in oxidation-reduction state and antioxidant enzymes in the roots of jack pine seedlings during cold acclimation. Physiologia Plantarum, 104, 134–142.CrossRefGoogle Scholar
Zhu, J.K. 2001. Plant salt tolerance. Trends in Plant Science, 6, 66–71.CrossRefGoogle ScholarPubMed
Zúñiga, G.E., Alberdi, M.Corcuera, L. 1996. Non-structural carbohydrates in Deschampsia antarctica Desv. from South Shetland Islands, maritime Antarctica. Environmental and Experimental Botany, 36, 396–399.CrossRefGoogle Scholar