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Variation of an indicator of Escherichia coli persistence from surface waters of mixed-use watersheds, and relationship with environmental factors

Published online by Cambridge University Press:  24 December 2010

Emilie Lyautey ,
Graham Wilkes ,
Jim J. Miller ,
Eric Van Bochove ,
Hans Schreier ,
Wendell Koning ,
Thomas A. Edge ,
David R. Lapen  and
Edward Topp
Emilie Lyautey
Affiliation:
Agriculture and Agri-Food Canada, 1391 Sandford St, London, ON N5V 4T3, Canada
Graham Wilkes
Affiliation:
Agriculture and Agri-Food Canada, 960 Carling Ave, Ottawa, ON K1A 0C6, Canada
Jim J. Miller
Affiliation:
Agriculture and Agri-Food Canada, 5403 1st Ave S, Lethbridge, AB T1J 4B1, Canada
Eric Van Bochove
Affiliation:
Agriculture and Agri-Food Canada, 2560 boul. Hochelaga, Sainte-Foy, QC G1V 2J3, Canada
Hans Schreier
Affiliation:
Institute for Resources, Environment and Sustainability, University of British Columbia, 429-2202 Main Mall, Vancouver, BC V6T 1Z4, Canada
Wendell Koning
Affiliation:
Alberta Environment, 2938-11 St. NE, Calgary, AB T2E 7L7, Canada
Thomas A. Edge
Affiliation:
Environment Canada, 867 Lakeshore Road, Burlington, ON L7R 4A6, Canada
David R. Lapen
Affiliation:
Agriculture and Agri-Food Canada, 960 Carling Ave, Ottawa, ON K1A 0C6, Canada
Edward Topp*
Affiliation:
Agriculture and Agri-Food Canada, 1391 Sandford St, London, ON N5V 4T3, Canada
*
*Corresponding author: [email protected]
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Abstract

Escherichia coli is an indicator of fecal pollution used to mandate recreational and drinking water quality. Concentrations of culturable E. coli following contamination of surface water are determined by three factors: dilution; cell attachment to particulate material and settling or resuspension in the water column; and the net rate of change in viability. This study evaluated the variability in the latter parameter, and how predictive variation in death rate was of culturable population densities at the time of sampling. Water samples (N=232) with varying levels of E. coli contamination were collected from 46 discrete locations in four watersheds across Canada over a three-month period and enumerated for culturable E. coli by membrane filtration plate counting (T0EC). Water samples were again enumerated following a laboratory 24 h holding period at 30°C in the dark, and the difference considered the death rate (ΔEC). Relationships of T0EC and ΔEC with environmental and water chemistry factors were explored using step-wise multiple regression. The model predicting T0EC indicated that stream order, total rainfall seven days in advance of sampling day, total phosphorus, and ΔEC were the most significant contributors. The model predicting ΔEC indicated that turbidity and NH3+NH4 were the most important contributors. A model suggests that the persistence factor is less important than dilution (i.e. stream order) in describing E. coli densities, followed by factors that influence the loading of E. coli into watersheds.

Keywords

Escherichia colisurface waterpersistenceenvironmental factors
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 47 , Issue 1 , 2011 , pp. 11 - 19
Copyright
© EDP Sciences, 2010

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References

American Public Health Association, 1999. Standard methods for the examination of water and wastewater, 20th edn., Clesceri, L.S, Greenberg, A.E and Eaton, A.D. (eds.), American Public Health Association, Washington, DC.
Anderson, K.L., Whitlock, J.E. and Harwood, V.J., 2005. Persistence and differential survival of fecal indicator bacteria in subtropical waters and sediments. Appl. Environ. Microbiol. , 71, 3041–3048.CrossRefGoogle ScholarPubMed
Benham, B.L., Baffaut, C., Zeckoski, R.W., Mankin, K.R., Pachepsky, Y.A., Sadeghi, A.M., Brannan, K.M., Soupir, M.L. and Habersack, M.J., 2006. Modeling bacteria fate and transport in watersheds to support TMDLs. T. ASABE , 49, 987–1002.CrossRefGoogle Scholar
Beversdorf, L.J., Bornstein-Forst, S.M. and McLellan, S.L., 2007. The potential for beach sand to serve as a reservoir for Escherichia coli and the physical influences on cell die-off. J. Appl. Microbiol. , 102, 1372–1381.CrossRefGoogle ScholarPubMed
Bouteleux, C., Saby, S., Tozza, D., Cavard, J., Lahoussine, V., Hartemann, P. and Mathieu, L., 2005. Escherichia coli behavior in the presence of organic matter released by algae exposed to water treatment chemicals. Appl. Environ. Microbiol. , 71, 734–740.CrossRefGoogle ScholarPubMed
Brettar, I. and Höfle, M.G., 1992. Influence of ecosystematic factors on survival of Escherichia coli after large-scale release into lake water mesocosms. Appl. Environ. Microbiol. , 58, 2201–2210.Google ScholarPubMed
Canale, R.P., Auer, M.T., Owens, E.M., Heidtke, T.M. and Effler, S.W., 1993. Modeling fecal-coliform bacteria. 2. Model development and application. Water Res. , 27, 703–714.CrossRefGoogle Scholar
Carlucci, A.F. and Pramer, P., 1960a. An evaluation of factors affecting the survival of Escherichia coli in sea water: III. Antibiotics. Appl. Environ. Microbiol. , 8, 251–254.Google Scholar
Carlucci, A.F. and Pramer, P., 1960b. An evaluation of factors affecting the survival of Escherichia coli in sea water: IV. Bacteriophages. Appl. Environ. Microbiol. , 8, 254–256.Google Scholar
Collins, R. and Rutherford, K., 2004. Modelling bacterial water quality in streams draining pastoral land. Water Res. , 38, 700–712.CrossRefGoogle ScholarPubMed
Cook, K.L. and Bolster, C.H., 2007. Survival of Campylobacter jejuni and Escherichia coli in groundwater during prolonged starvation at low temperatures. J. Appl. Microbiol. , 103, 573–583.CrossRefGoogle ScholarPubMed
Craig, D.L., Fallowfield, H.J. and Cromar, N.J., 2004. Use of microcosms to determine persistence of Escherichia coli in recreational coastal water and sediment and validation with in situ measurements. J. Appl. Microbiol. , 96, 922–930.CrossRefGoogle ScholarPubMed
Davis, R.K., Hamilton, S. and Van Brahana, J., 2005. Escherichia coli survival in mantled karst springs and streams, northwest Arkansas Ozarks, USA. J. Am. Water Resour. Assoc. , 41, 1279–1287.CrossRefGoogle Scholar
Dufour, A.P., 1984. EPA health effects criteria for fresh recreational waters, United States Environmental Protection Agency, Research Triangle Park, NC.Google Scholar
Environment Canada, 2007. National climate data and information archive, Environment Canada, Ottawa, ON.
Faust, M.A., Aotaky, A.E. and Hargadon, M.T., 1975. Effect of physical parameters on the in situ survival of Escherichia coli MC 6 in an estuarine environment. J. Appl. Microbiol. , 30, 800–806.Google Scholar
Flint, K.P., 1987. The long-term survival of Escherichia coli in river water. J. Appl. Bacteriol. , 63, 261–270.CrossRefGoogle ScholarPubMed
Haas, C.N., Rose, J.B. and Gerba, C.P., 1999. Quantitative Microbial Risk Assessment, John Wiley and Sons, Inc., New York, NY.Google Scholar
Ishii, S. and Sadowsky, M.J., 2008. Escherichia coli in the environment: implications for water quality and human health. Microbes Environ. , 23, 101–108.CrossRefGoogle ScholarPubMed
Ishii, S., Ksoll, W.B., Hicks, R.E. and Sadowsky, M.J., 2006. Presence and growth of naturalized Escherichia coli in temperate soils from Lake Superior watersheds. Appl. Environ. Microbiol. , 72, 612–621.CrossRefGoogle ScholarPubMed
Ishii, S., Hansen, D.L., Hicks, R.E. and Sadowsky, M.J., 2007. Beach sand and sediments are temporal sinks and sources of Escherichia coli in Lake Superior. Environ. Sci. Technol. , 41, 2203–2209.CrossRefGoogle Scholar
Johnson, J.Y.M., Thomas, J.E., Graham, T.A., Townshend, I., Byrne, J., Selinger, L.B. and Gannon, V.P.J., 2003. Prevalence of Escherichia coli O157:H7 and Salmonella spp. in surface waters of southern Alberta and its relation to manure source. Can. J. Microbiol. , 49, 326–335.CrossRefGoogle Scholar
Kashefipour, S.M., Lin, B. and Falconer, R.A., 2002. Dynamic modelling of bacterial concentrations in coastal waters: effects of solar radiation on decay. Adv. Hydraul. Water Eng. , 1–2, 993–998.CrossRefGoogle Scholar
Leclerc, H., Mossel, D.A.A., Edberg, S.C. and Struijk, C.B., 2001. Advances in the bacteriology of the coliform group: their suitability as markers of microbial water safety. Annu. Rev. Microbiol. , 55, 201–234.CrossRefGoogle ScholarPubMed
Marshall, K.C., 1968. Interaction between colloidal montmorillonite and cells of Rhizobium species with different inorganic surfaces. Biochim. Biophys. Acta , 156, 179–186.CrossRefGoogle Scholar
McCambridge, J. and McMeekin, T.A., 1980. Relative effects of bacterial and protozoan predators on survival of Escherichia coli in estuarine water samples. Appl. Environ. Microbiol. , 40, 907–911.Google ScholarPubMed
Muela, A., Garcia-Bringas, J.M., Arana, I. and Barcina, I., 2000. The effect of simulated solar radiation on Escherichia coli: the relative roles of UV-B, UV-A, and photosynthetically active radiations. Microb. Ecol. , 39, 65–71.CrossRefGoogle Scholar
Noble, R.T., Lee, I.M. and Schiff, K.C., 2004. Inactivation of indicator micro-organisms from various sources of faecal contamination in seawater and freshwater. J. Appl. Microbiol. , 96, 464–472.CrossRefGoogle ScholarPubMed
Pachepsky, Y.A., Sadeghi, A.M., Bradford, S.A., Shelton, D.R., Guber, A.K. and Dao, T., 2006. Transport and fate of manure-borne pathogens: modeling perspective. Agr. Water Manage. , 86, 1–92.CrossRefGoogle Scholar
Roser, D.J., Davies, C.M., Ashbolt, N.J. and Morison, P., 2006. Microbial exposure assessment of an urban recreational lake: a case study of the application of new risk-based guidelines. Water Sci. Technol. , 54, 245–252.CrossRefGoogle ScholarPubMed
Servais, P., Billen, G. and Rego, J.V., 1985. Rate of bacterial mortality in aquatic environments. Appl. Environ. Microbiol. , 49, 1448–1454.Google ScholarPubMed
Servais, P., Garcia-Armisen, T., George, I. and Billen, G., 2007. Fecal bacteria in the rivers of the Seine drainage network (France): sources, fate and modelling. Sci. Total Environ. , 375, 152–167.CrossRefGoogle ScholarPubMed
Smith, I.M., Hall, K.J., Lavkulich, L.M. and Schreier, H., 2007. Trace metal concentrations in an intensive agricultural watershed in British Columbia, Canada. J. Am. Water Resour. Assoc. , 43, 1455–1467.CrossRefGoogle Scholar
Strahler, A.N., 1952. Hypsometric (area altitude) analysis of erosional topology. Geol. Soc. Am. Bull. , 63, 1117–1142.CrossRefGoogle Scholar
Topp, E., Welsh, M., Tien, Y.C., Dang, A., Lazarovits, G., Conn, K. and Zhu, H., 2003. Strain-dependent variability in growth and survival of Escherichia coli in agricultural soil. FEMS Microbiol. Ecol. , 44, 303–308.CrossRefGoogle ScholarPubMed
U.S. Environmental Protection Agency (USEPA), 2000. Improved enumeration methods for the recreational water quality indicators: Enterococci and Escherichia coli, Office of Science and Technology, Washington, DC.
van Bochove, E., Dechmi, F., Nolin, M.C., Chantigny, M.H., Lemieux, C., Thériault, G. and Corriveau, J., 2005. Evaluation of beneficial agricultural management practices on water quality at edge-of-field and micro-watershed scales in Southern Québec, Canada. Proceedings of the International Water Association, 9th Diffuse Pollution Conference, Johannesburg, South Africa.
Walk, S.T., Alm, E.W., Calhoun, L.M., Mladonicky, J.M. and Whittam, T.S., 2007. Genetic diversity and population structure of Escherichia coli isolated from freshwater beaches. Environ. Microbiol. , 9, 2274–2288.CrossRefGoogle ScholarPubMed
Weinbauer, M.G. and Höfle, M.G., 1998. Significance of viral lysis and flagellate grazing as factors controlling bacterioplankton production in a eutrophic lake. Appl. Environ. Microbiol. , 64, 431–438.Google Scholar
Whitman, R.L., Przybyla-Kelly, K., Shively, D.A., Nevers, M.B. and Byappanahalli, M.N., 2008. Sunlight, season, snowmelt, storm, and source affect E. coli populations in an artificially ponded stream. Sci. Total Environ. , 390, 448–455.CrossRefGoogle Scholar
Wilkes, G., Edge, T., Gannon, V., Jokinen, C., Lyautey, E., Medeiros, D., Neumann, N., Ruecker, N., Topp, E. and Lapen, D.R., 2009. Seasonal relationships among indicator bacteria, pathogenic bacteria, Cryptosporidium oocysts, and Giardia cysts in surface waters within an agricultural landscape. Water Res. , 43, 2209–2223.CrossRefGoogle ScholarPubMed
Wilkinson, J., Jenkins, A., Wyer, M. and Kay, D., 1995. Modeling fecal-coliform dynamics in streams and rivers. Water Res. , 29, 847–855.CrossRefGoogle Scholar
Wommack, K.E. and Colwell, R.T., 2000. Virioplankton: viruses in aquatic ecosystems. Microbiol. Mol. Biol. Rev. , 64, 69–114.CrossRefGoogle ScholarPubMed