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Using high-pressure teflon bomb digestion in phosphorus determination of aquatic animals

Published online by Cambridge University Press:  03 April 2009

Gergely Boros ,
István Tátrai  and
Sándor A. Nagy
Gergely Boros*
Affiliation:
Balaton Limnological Research Institute of the Hungarian Academy of Sciences, 8237, P.O. Box 35, Tihany, Hungary Department of Hydrobiology, University of Debrecen, 4032, Egyetem tér 1., Debrecen, Hungary
István Tátrai
Affiliation:
Balaton Limnological Research Institute of the Hungarian Academy of Sciences, 8237, P.O. Box 35, Tihany, Hungary
Sándor A. Nagy
Affiliation:
Department of Hydrobiology, University of Debrecen, 4032, Egyetem tér 1., Debrecen, Hungary
*
[email protected]
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Abstract

High pressure and temperature were combined with the convenient mixture of acids to perform an effective digestion of samples from aquatic animals for phosphorus (P) analysis. Our results indicate that digestion in high-pressure bombs combined with concentrated acidic media can be a convenient way to yield all P stored in samples, and to produce accurate recoveries. Phosphorus recovery of a certified standard material (94% ± 15) and digestions of aquatic animal tissues confirmed the relevance of bomb decomposition, as well as its reliability compared to ashing and microwave oven digestion methods. Moreover, the applied digestion technique is easily manageable even in samples consisting of a few individuals. This method is relatively rapid, saves money and time, and is able to recover P from the most persistent components (e.g. bones, scales, fat) of aquatic animal tissues.

Keywords

High-pressure digestionphosphorusfishzooplanktonbenthos
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 45 , Issue 1 , 2009 , pp. 55 - 58
Copyright
© EDP Sciences, 2009

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References

Andersen, T. and Hessen, D.O., 1991. Carbon, nitrogen, and phosphorus content of freshwater zooplankton. Limnol. Oceanogr. , 36, 807-814. CrossRef
Brown, H.G., Hensley, C.P. and Thale, J.E., 1973. Low-temperature digestion method for fish tissue and sediment prior to total mercury analysis. Transactions of the Kansas Academy of Science. , 76, 4-8. CrossRef
Colina, M., Ledo, H., Gutiérrez, E., Villalobos, E. and Marín, J., 1996. Determination of total phosphorus in sediments by means of high-pressure bombs and ion chromatography. J. Chromatogr. , 739, 223-227. CrossRef
Farkas, A., Salánki, J. and Specziár, A., 2002. Relation between growth and the heavy metal concentration in organs of bream Abramis brama L. populating Lake Balaton. Arch. Environ. Contam. Toxicol. , 43, 236-243. CrossRef
Farkas, A., Salánki, J. and Specziár, A., 2003. Age- and size specific patterns of heavy metals in the organs of freshwater fish Abramis brama L. populating a low-contaminated site. Wat. Res. , 37, 959-964. CrossRef
Frost, P.C., Tank, S.E., Turner, M.A. and Elser, J.J., 2003. Elemental composition of littoral invertebrates from oligortophic and eutrophic Canadian lakes. J. N. Am. Benthol. Soc. , 22, 51-62. CrossRef
Going, J.E. and Eisenreich, S.J., 1974. Spectrophotometric studies of reduced molybdoantimonylphosphoric acid. Anal. Chim. Acta , 70, 95-106. CrossRef
Hendrixson, H.A., Sterner, R.W. and Kay, A.D., 2007. Elemental stoichiometry of freshwater fishes in relation to phylogeny, allometry and ecology. J. Fish Biol. , 70, 121-140. CrossRef
Higgins, K.A., Vanni, M.J. and González, M.J., 2006. Detritivory and the stoichiometry of nutrient cycling by a dominant fish species in lakes of varying productivity. Oikos , 114, 419-430. CrossRef
Jarvie, H.P., Withers, P.J.A. and Neal, C., 2002. Review of robust measurement of phosphorus in river water: sampling, storage, fractionation and sensitivity. Hydrol. Earth Sys. Sci. , 6, 113-132. CrossRef
Le Loc'h, F., Hily, C. and Grall, J., 2008. Benthic community and food web structure on the continental shelf of the Bay of Biscay (North Eastern Atlantic) revealed by stable isotopes analysis. J. Marine Syst. , 72, 17-34. CrossRef
Pai, S., Yang, C.C. and Riley, J.P., 1990. Effects of acidity and molybdate concentration on the kinetics of the formation of the phosphoantimonylmolybdenum blue complex. Anal. Chim. Acta , 229, 115-120.
Penczak, T. and Tátrai, I., 1985. Contribution of bream, Abramis brama (L.), to the nutrient dynamics of Lake Balaton. Hydrobiologia , 126, 59-64. CrossRef
Pilati, A. and Vanni, M.J., 2007. Ontogeny, diet shifts, and nutrient stoichiometry in fish. Oikos , 116, 1663-1674. CrossRef
Reis, P.A., Valente, L.M.P. and Almeida, C.M.R., 2008. A fast and simple methodology for determination of yttrium as an inert marker in digestibility studies. Food Chem. , 108, 1094-1098. CrossRef
Ringmann, S., Boch, K., Marquardt, W., Schuster, M., Schlemmer, G. and Kainrath, P., 2002. Microwave-assisted digestion of organoarsenic compounds for the determination of total arsenic in aqueous, biological, and sediment samples using flow injection hybride generation electrothermal atomic absorption spectrometry. Anal. Chim. Acta , 452, 207-215. CrossRef
Sadiq, M. and Zaidi, T.H., 1983. A study of various factors affecting digestion of fish tissue prior to mercury determination. Int. J. Environ. Anal. Chem. , 16, 57-66. CrossRef
Schnitzer, G., Soubelet, A., Testu, C. and Chafey, C., 1995. Comparison of open and closed focused microwave digestions in view of total mercury determination by cold vapour atomic absorption spectrometry. Microchim. Acta. , 119, 199-209. CrossRef
Sereda, J.M., Hudson, J.J., Taylor, W.D. and Demers, E., 2008. Fish as sources and sinks of nutrients in lakes. Freshwat. Biol. , 53, 278-289.
Sterner, R.W. and George, N.B., 2000. Carbon, nitrogen and phosphorus stoichiometry of cyprinid fishes. Ecology , 81, 127-140. CrossRef
Strickland J.D.H. and Parsons T.R., 1972. A practical handbook of seawater analysis. Fish. Res. Board Can. Bull., 167, Ottawa, Canada, 310 p.
Tanner, D.K., Leonard, E.N. and Brazner, J.C., 1999. Microwave digestion method for phosphorus determination of fish tissue. Limnol. Oceanogr. , 44, 708-709. CrossRef
Tanner, D.K., Brazner, J.C. and Brady, V.J., 2000. Factors influencing carbon, nitrogen, and phosphorus content of fish from a Lake Superior coastal wetland. Can. J. Fish. Aquat. Sci. , 57, 1243-1251. CrossRef
Walve, J. and Larsson, U., 1999. Carbon, nitrogen and phosphorus stoichiometry of crustacean zooplankton in the Baltic Sea: implications for nutrient recycling. J. Plank. Res. , 21, 2309-2321. CrossRef