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Initial growth phases of two bloom-forming cyanobacteria (Cylindrospermopsis raciborskii and Planktothrix agardhii) in monocultures and mixed cultures depending on light and nutrient conditions

Published online by Cambridge University Press:  17 July 2014

Myriam Ammar ,
Katia Comte ,
Thi Du Chi Tran  and
Monia El Bour
Myriam Ammar
Affiliation:
Laboratoire de Pathologies des Animaux Aquatiques, Institut National des Sciences et Technologies de la Mer, 2025 Salammbô, Tunisia UMR 7245 CNRS-MNHN Molécules de communication et adaptation des micro-organismes, Muséum national d'Histoire naturelle, Case 39, 12, rue Buffon, F-75231 Paris cedex 05, France
Katia Comte*
Affiliation:
UMR 7245 CNRS-MNHN Molécules de communication et adaptation des micro-organismes, Muséum national d'Histoire naturelle, Case 39, 12, rue Buffon, F-75231 Paris cedex 05, France
Thi Du Chi Tran
Affiliation:
UMR 7245 CNRS-MNHN Molécules de communication et adaptation des micro-organismes, Muséum national d'Histoire naturelle, Case 39, 12, rue Buffon, F-75231 Paris cedex 05, France Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi, Vietnam
Monia El Bour
Affiliation:
Laboratoire de Pathologies des Animaux Aquatiques, Institut National des Sciences et Technologies de la Mer, 2025 Salammbô, Tunisia
*
*Corresponding author: [email protected]
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Abstract

Proliferations of cyanobacteria have detrimental effects on ecosystem functioning, and on the global freshwater food chain. Many studies have focused on the “in situ” dynamics of bloom-forming cyanobacteria, including Cylindrospermopsis raciborskii and Planktothrix agardhii. Few have used experimental assays to explore the fast-growing ability of naturally co-occurring species. Here we investigated the growth of these species when exposed separately (i.e., in monocultures) to a range of light and nutrient conditions, plus their interactive performances in mixed cultures in a short-time experiment (6 days). The use of microplates made it possible to carry out multiple measurements of in-vivo fluorescence (IVF), and to monitor species-dependent biovolumes. No allelopathic effect was significantly observed for any target species, while significantly lower growth rates were obtained in mixed cultures, which may reflect other interference interactions between the species. We showed that Planktothrix grew faster with low light intensity and high nutrient concentrations, and was drastically inhibited by nitrogen deprivation, in contrast to Cylindrospermopsis. However, Cylindrospermopsis outgrew Planktothrix at high NH4+ concentrations, suggesting that this species may be a serious competitor for the native species in many water systems.

Keywords

Bloom-forming cyanobacteriainteractionsgrowth ratemixed cultures
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 50 , Issue 3 , 2014 , pp. 231 - 240
Copyright
© EDP Sciences, 2014

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