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A heterotrophic nanoflagellate grazing on the toxic Cyanobacterium Microcystis aeruginosa

Published online by Cambridge University Press:  03 April 2009

Cui Yan ,
Jian-Hong Li ,
Ju-Jiao Li ,
Jin Wang  and
Yong-Ping Weng
Cui Yan
Affiliation:
Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210046, P. R. China
Jian-Hong Li*
Affiliation:
Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210046, P. R. China
Ju-Jiao Li
Affiliation:
Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210046, P. R. China
Jin Wang
Affiliation:
Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210046, P. R. China
Yong-Ping Weng
Affiliation:
Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210046, P. R. China
*
[email protected]
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Abstract

Cyanobacterial blooms cause extensive ecological damages in aquatic environments. Heterotrophic nanoflagellates (HNF) play an important role in controlling the populations of cyanobacteria in natural water bodies. In this study, we report a HNF, NF-WJ05, which grazes efficiently on the toxic cyanobacterium Microcystis aeruginosa strain PCC 7806. The morphological characteristics of the nanoflagellate observed by optical microscope and confocal microscope showed that NF-WJ05 could be a Paraphysomonas. The sequences of the internal transcribed spacer (ITS) regions of rDNA including the 5.8S rDNA region was determined and compared with sequences available in databases. The 5.8S rDNA sequence showed a high degree of similarity to those belonging to species of Chromophyta. However, sequences similar to that of its ITS were not found in the databases. Several environmental factors affecting the grazing efficiency of NF-WJ05 on cyanobacteria were evaluated. The more suitable conditions for grazing were 30°C and pH 5.0 with stirring. Ammonia inhibited the grazing, whereas low concentrations of phenol increased the grazing rate with an optimal concentration at 50 µg.L-1.

Keywords

CyanobacteriagrazerITS sequenceMicrocystis aeruginosananoflagellate
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 45 , Issue 1 , 2009 , pp. 23 - 28
Copyright
© EDP Sciences, 2009

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References

Boenigk, J., Pfandl, K., Stadler, P. and Chatzinotas, A., 2005. High diversity of the ‘Spumella-like’ flagellates: an investigation based on the SSU rRNA gene sequences of isolates from habitats located in six different geographic regions. Environ. Microbiol. , 7, 685-697. CrossRef
Callieri, C., Karjalainen, S.M. and Passoni, S., 2002. Grazing by ciliates and heterotrophic nanoflagellates on picocyanobacteria in Lago Maggiore, Italy. J. Plankton Res. , 24, 785-796. CrossRef
Chorus I. and Bartram J., 1999. Toxic cyanobacteria in water: A guide to their public health consequences, monitoring and management, Published on Behalf of WHO. F and FN Spon.
Christaki, U., Vazquez-Dominguez, E., Courties, C. and Lebaron, P., 2005. Grazing impact of different heterotrophic nanoflagellates on eukaryotic (Ostreococcus tauri) and prokaryotic picoautotrophs (Prochlorococcus and Synechococcus). Environ. Microbiol. , 7, 1200-1210. CrossRef
Cole, G.T. and Wynne, M.J., 1974. Endocytosis of Microcystis aeruginosa by Ochromonas danica. J. Phycol. , 10, 397-410.
Dokulil, M.T. and Teubner, K., 1998. Cyanobacterial dominance in eutrophic lakes: Causes-Consequences-Solutions. J. Lake Sci. , 10, 357-370.
Dolan, J.R. and Šimek, K., 1998. Ingestion and digestion of an autotrophic picoplankter, Synechococcous, by a heterotrophic nanoflagellate, Bodo saltans. Limnol. Oceanogr. , 43, 1740-1746. CrossRef
Dolan, J.R. and Šimek, K., 1999. Diel periodicity in Synechococcus populations and grazing by heterotrophic nanoflagellates: Analysis of food vacuole contents. Limnol. Oceanogr. , 44, 1565-1570. CrossRef
Goodwin, S.B., Dunkle, L.D. and Zismann, V.L., 2001. Phylogenetic analysis of Cercospora and Mycosphaerella based on the internal transcribed spacer region of ribosomal DNA. Phytopathology , 91, 648-658. CrossRef
Hallegreff, G.M., 1993. A review of harmful algal bloom and their apparent global increase. Phycologia , 32, 79-99. CrossRef
Hitzfeld B.C., Hoger S.J. and Dietrich D.R., 2000. Cyanobacterial toxins: Removal during drinking water treatment and human risk assessment. Environ. Health Perspect., 108, Suppl. 1, 113-122.
Honkanen, R.E., Zwiller, J., Mooren, R.E., Daily, S.L., Khatrall, B.S., Dukelow, M. and Boynton, A.L., 1990. Characterization of microcystin-LR, a potent inhibitor of type 1 and type 2A protein phosphatases. J. Biol. Chem. , 265, 19401-19404.
IPCS, 1994. Phenol health and safety guide, health and safety guide n° 88, International Programme on Chemical Safety (IPCS), Environmental Health Criteria 161, World Health Organization, Geneva.
Klaveness, D., 1995. Collodictyon triciliatum H.J. Carter (1865) - a common but fixation-sensitive algivorous flagellate from the limnopelagial. Nordic J. Freshw. Res. , 70, 3-11.
Kong, F.Q. and Gao, G., 2005. Hypothesis on cyanobacteria bloom-forming mechanism in large shallow eutrophic lakes. Acta Ecol. Sinica , 25, 589-595.
Kumar, S., Tamura, K. and Nei, M., 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. , 5, 150-163. CrossRef
Mariottini, G.L. and Pane, L., 2003. Ecology of planktonic heterotrophic flagellates, a review. Riv. Biol. , 96, 55-71.
Nishibe, Y., Kawabata, Z. and Nakano, S., 2002. Grazing on Microcystis aeruginosa by the heterotrophic flagellate Collodictyon triciliatum in a hypertrophic pond. Aquat. Microb. Ecol. , 29, 173-179. CrossRef
Ochs, C.A. and Eddy, L.P., 1998. Effects of UV-A (320 to 399 nanometers) on grazing pressure of a marine heterotrophic nanoflagellate on strains of the unicellular cyanobacteria Synechococcus spp. Appl. Environ. Microbiol. , 64, 287-293.
Paerl, H.W., Fulton, R.S. III., Moisander, P.H. and Dyble, J., 2001. Harmful freshwater algal blooms, with an emphasis on cyanobacteria. Sci. World J. , 1, 76-113. CrossRef
Pernthaler, J., Šimek, K., Sattler, B., Schwarzenbacher, A., Bobkova, J. and Psenner, R., 1996. Short-term changes of protozoan control on autotrophic picoplankton in an oligo-mesotrophic lake. J. Plankton. Res. , 18, 443-462. CrossRef
Randall, D.J. and Tsui, T.K., 2002. Ammonia toxicity in fish. Mar. Pollut. Bull. , 45, 17-23. CrossRef
Redecker, D., Hijri, M., Dulieu, H. and Sanders, I.R., 1999. Phylogenetic analysis of a dataset of fungal 5.8S rDNA sequences shows that highly divergent copies of internal transcribed spacers reported from Scutellospora castanea are of Ascomycete origin. Fungal Genet. Biol. , 28, 238-244. CrossRef
Rippka, R., Deruelles, J.D., Waterbury, J., Herdman, M. and Stanier, R., 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. , 111, 1-61.
Sherr, E.B. and Sherr, B.F., 1994. Bacterivory and herbivory-Key roles of phagotrophic protests in pelagic food webs. Microbial Ecol. , 28, 223-235. CrossRef
Sigee D.C., Glenn R., Andrews M.J., Bellinger E.G., Butler R.D., Epton H.A.S. and Hendry R.D., 1999. Biological control of cyanobacteria: principles and possibilities. Hydrobiologia, 395/396, 161-172.
Šimek, K. and Chrzanowski, T.H., 1992. Direct and indirect evidence of size-selective grazing on pelagic bacteria by freshwater nanoflagellates. Appl. Environ. Microbiol. , 58, 3715-3720.
Sivonen K. and Jones G., 1999. Cyanobacterial toxins. In: Chorus I., Bartram J. (eds.), Toxic Cyanobacteria in Water, E and FN Spon London, London, UK, 41-111.
Sugiura, N., Inamori, Y., Ouchiyama, T. and Sudo, R., 1992. Degradation of cyanobacteria, Microcystis by microflagellate, Monas guttula. Water Sci. Technol. , 26, 2173-2176.
Vardi, A., Schatz, D., Beeri, K., Motro, U., Sukenik, A., Levine, A. and Kaplan, A., 2002. Dinoflagellate-cyanobacterium communication may determine the composition of phytoplankton assemblage in a mesotrophic lake. Curr. Biol. , 12, 1767-1772. CrossRef
Yoo S., Carmichael W.W., Hoehn R.C. and Hrudey S.E., 1995. Cyanobacterial (Blue-green Algal) toxins: a resource guide, American Water Works Association Research Foundation, Denver, Colorado.
Zhang, X., Watanabe, M.M. and Inouye, I., 1996. Light and electron microscopy of grazing by Poterioochromonas malhamensis (Chrysophyceae) on a range of phytoplankton taxa. J. Phycol. , 32, 37-46. CrossRef