Polyamines (putrescine, spermidine and spermine) are required for optimal growth in all cells, and are essential for cell proliferation and growth of cultured wool follicles, with an optimal concentration of spermidine required for the fibre elongation. The effects of a local supply of exogenous spermidine on the rate of cell division in the wool follicles, the length growth rate and diameter of fibres were therefore examined in Merino lambs. Three groups of eight lambs (40 kg) were given food at 1·2 ✕ maintenance. Spermidine was injected intradermally into a small patch (3 ✕ 3 cm) on the left flank three times per day for 7 days at one of three concentrations: 1·38, 2·75 or 4·58 џmol in 0·8 ml volume. The same volume of saline was injected into the contralateral side as a control. The concentration of spermidine in the skin patch 3 h after injection on day 7 increased by proportionately 018, 0·33 or 0·41 (P < 0001) respectively. The rates of cell division in the follicle bulb 3 h after the spermidine injection were proportionately 0104, 0184 and 0·283 higher compared with the contralateral side (P = 0078 overall) for the low, medium and high doses of spermidine respectively and differed between the three doses (P < 005). The fibre length growth rate, as measured using autoradiography, was proportionately 0099, 0117 and 0156 higher than that of the contralateral side (P < 0001 overall) for the low, medium and high doses of spermidine respectively, but differences between doses were not significant (P > 005). Spermidine injection did not result in a significant change in fibre diameter during the treatment period. The ratio of fibre length growth rate to fibre diameter was increased by the injection of spermidine (P < 0001). The results suggest that injecting extra spermidine into the skin altered spermidine homeostasis in the skin, stimulated cell proliferation and resulted in increased fibre growth.

In ruminants and humans, the majority of whole body glucose utilization is not mediated by insulin. However, while in man most non-insulin-mediated glucose utilization (NIMGU) occurs in the brain, in ruminants the locations of NIMGU remain less well defined. As fasting would be expected to limit NIMGU to what would be regarded as an essential minimum, two studies were performed to establish the contribution of NIMGU to total glucose metabolism in fed and fasted sheep. Each study used four adult castrated male sheep. In study 1, a primed continuous infusion of U- [13C] glucose was begun at time 0 and continued for 7 h. After 3 h of isotope infusion (basal period) somatostatin (0•417 µg/kg per min; SS) was administered for 4 h, either alone (SS-only) or together with insulin (1•0 mU/kg per min; SS + insulin) with normal glucose to maintain euglycaemia for 2 h. Normal glucose was then infused for both the SS-only and SS + insulin treatments to induce and maintain hyperglycaemia over the final 2 h of the experiment. In study 2, fed or 72-h fasted sheep were infused with 6-[3H] glucose from time 0 for 8 h, with SS infusion starting at 3 h and continuing for 5 h. After 3 h of SS infusion, glucose was infused to induce and maintain hyperglycaemia. In both studies SS infusion inhibited insulin secretion, however in study 2, SS in fed sheep caused hyperglycaemia; this effect was not significant in the fasted animals. The rate of glucose utilization was reduced by SS-only as it eliminated insulin mediated glucose uptake (IMGU); under such conditions whole body glucose disposal should be NIMGU. In fed sheep, average NIMGU levels represented between proportionately 0•61 and 0•67 of the basal glucose metabolic clearance rate. During the infusion of SS + insulin in fed sheep, NIMGU fell to 0•34 during euglycaemia and 0•33 during hyperglycaemia, as the infused insulin caused IMGU to predominate. In fasted sheep the absolute rates of both IMGU and NIMGU were reduced, though NIMGU as a proportion of total turn-over (IMGU + NIMGU) increased to 0•88 of glucose metabolic clearance. Calculations suggest that, in contrast to man, only a minor proportion of NIMGU is utilized by the brain and central nervous system in fed or fasted sheep. It is suggested that skeletal muscle and the gastro-intestinal tract may make a major contribution to NIMGU, even in fasted sheep.

The birth weight and growth rate of sucking calves reared by three groups of cows grazing on veld and receiving different amounts of concentrates during winter and the first 8 weeks of lactation were studied over four successive calving seasons. Calves were weaned at either 150 or 240 days. The efficiency of use of concentrates for weaned calf production was calculated.

Calves from cows given most concentrates were heavier (P<0·01) than othei calves at birth, 150 days and 240 days. Milk consumption during the first 98 days of life accounted for most of the variation in pre-weaning growth. Time of weaning had little effect (P<0·05) on birth weight of the next year's calf and although cows in the early- weaning groups produced calves which were the heaviest at 150 days (P>0·05) these calves weighed less than other calves at 240 days (P<0·001). Simple and multiple regression analyses were used to show that birth weight was affected significantly by cow-weight change during winter and these differences were in part independent of treatment and year. Calf birth weight increased as age of dam increased but this was due to increase in weight of cow with age. Within classes, calf birth weight had a significant and positive effect on weight gain after accounting for milk consumption and this was shown to be an effect of sex. Cows given concentrates produced more weight of 240-days-old weaned calf per year than those given none (P<0·01), and late weaning led to the production of more weaned calf per annum than early weaning (P< 0·001) even when allowance was made for differences in concentrate consumption.

A deterministic method of predicting numbers of each animal class present over time in a beef cow herd with multiple calving seasons per year is described. Breeding cow classes are defined according to the number of generations of descent from the cows in the herd prior to the introduction of a specific heifer replacement breeding policy. Cow classes are further divided into subgroups defined according to age of cow, calving interval and whether or not the cow was last mated to a terminal sire. The method is demonstrated by showing the evolution of herd breed make-up with several alternative replacement female breeding policies including grading-up, two-breed rotation and three-breed rotation breeding policies. For an example herd, the first generation of replacements peaked at approximately 0·4 of the herd at 6 years after the first matings for a new breeding system. Later generations of replacements reached equivalent proportions of the herd to the previous generation approximately 3·3 years later, with peak proportions at approximately 0·25 of the herd. Differences in the relative levels of expression of heterosis for grade up versus rotational replacement policies increased steadily from 5 years onwards with 0·17 of the heterosis expected in a herd of F1 cows remaining 20 years after the first matings of the new breeding system. The two-breed grading-up system showed less heterosis than the three-breed grading-up system only after 8 years, with the difference increasing to 0·16 of the heterosis in an F1 herd after 20 years. Results are also presented showing the difference in contributions of breeds to the herd gene pool over time with the different breeding systems. With the rotational systems, breeds lag by approximately 3 years in the timing of their contribution according to the order of their introduction to the rotation. The effects of faster heifer replacement rates on results were also discussed. It is concluded that short-to medium-term impacts of breed and replacement breeding system choices are likely to be more relevant in practical decision making by suckler herd managers than the characteristics of breeding systems once herds have reached genetic equilibrium.

The present study was conducted to investigate the effects of ghrelin on gastric acid and pepsin secretion, as well as the mRNA expression of gastrin, somatostatin (SS) and receptors for growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in gastric mucosal cells in vitro. Gastric mucosal cells were isolated from 5-week-old weanling piglets and exposed for 4 h to 3×10−2, 3×10−1, 3, 3×10 and 3×102 nmol/l of h-ghrelin, respectively after 30-h incubation in DMEM/F-12. Pepsin activity in culture medium, cell viability and H+-K+-ATPase activity, as well as mRNA expression of gastrin, SS, GHR and IGF-1R in gastric mucosal cells were determined as response criteria. The experiment was repeated three times. Ghrelin significantly (P<0·05) increased H+-K+-ATPase activity of gastric mucosal cells at 3×10−1, 3 and 3×10 nmol/l of h-ghrelin. However, no significant changes were observed either in pepsin activity or the cell viability after ghrelin treatment. The mRNA expression of gastrin and SS was significantly increased in gastric mucosal cells exposed to h-ghrelin at 3×10−1 and 3 nmol/l (P<0·05). H-ghrelin significantly increased IGF-1R but not GHR mRNA expression at 3×10−1, 3 and 3×10 nmol/l of h-ghrelin (P<0·05). Ghrelin acts on gastric mucosal cells from weanling piglets to regulate the H+-K+-ATPase activity and mRNA expression of gastrin, SS, and IGF-1R in vitro.

In a previous experiment the provision of straw to group-housed pigs increased the number of daily feeder visits. This effect of straw is comparable with conditions of reduced social competition for access to the feeder. The aim of this experiment was to investigate if changing straw provision within a pen resulted in similar and reversible effects in this behaviour. One hundred and twenty male pigs (41·1 (s.e. 0·43) kg) were used in three blocks each of four pens of 10 pigs. In each block of 42 days duration one pen had no straw throughout (treatment 1); one pen had no straw for 21 days (period 1) followed by straw for 21 days (period 2) (2); one pen had straw followed by no straw (3) and one pen had straw throughout (4). The pens were situated in a naturally ventilated building with light from 06.00 to 20.00 h. Each pen had a kennel, a lying area and a single-space computerized feeder for recording feeding behaviour. The pigs’ activity was recorded on video in the 2nd and 5th weeks of each block. The number of feeder visits was slightly higher for pigs with straw (1: 9·69 v. 4: 10·46) but this was not significant (P > 0·05). The effect was more marked for treatment 2 where the visits increased (P < 0·01) from period 1 (8·52) to period 2 (10·64) and for treatment 3 where they decreased (11·20 v. 10·10). Pigs without straw (treatment 1) spent more time in the kennel than those with straw (treatment 4) (P < 0·05). For treatment 2 the proportion of time spent in the kennel fell from 0·607 in period 1 to 0·288 in period 2 (P < 0·01). There was a general trend for more activity and more lying outside the kennel when straw was provided. The effect of straw provision was not as marked as that seen previously; nevertheless changing straw provision (treatments 2 and 3) did result in an increase in feeder visits with straw, probably related to the increase in general activity and lying outside in the area adjacent to the feeder.

Two temperature regimes were compared in relation to the growth and feed conversion ratio of groups of cage-reared pigs between 10 and 38 days of age. The treatments were: 27°C at 10 days reducing by 1°C every 4 days to 20°C at 38 days; and 27°C at 10 days reducing by 1°C daily to 20°C at 17 days and then remaining constant. Twelve replicates using 244 pigs were completed, the group size varying between 9 and 12 pigs. The mean growth rates on the two temperature regimes were respectively 234 and 243 g/day, and in both cases feed conversion ratio (kg DM/kg gain) averaged 1·03. It is concluded that temperatures lower than those currently recommended for pigs weaned at 14 to 16 days into controlled-environment houses would support satisfactory performance with direct savings in heating costs under winter conditions.

Observations of the behaviour of confined sows provide information about how difficult it is for each individual to cope with the conditions in which it lives. Behaviour which has been described as occurring more frequently in confined sows than in sows with space to walk around includes stereotypies and apathetic behaviour. A stereotypy is a relatively invariate sequence of movements occurring so frequently in a particular context that it could not be considered to form part of one of the normal functional systems of the animal, feeding, body maintenance, reproduction, etc. (Broom 1983). The term apathetic behaviour has generally implied inactivity and lack of responsiveness. In this study, carried out at the Animal and Grassland Research Institute, the occurrence of stereotypies and the degree of responsiveness of stall-housed sows have been quantified.

At farrowing time, sows are normally confined in barren environments which prevent the performance of normal behaviour patterns. It has been argued that both the sows welfare and productivity may suffer as a result of these conditions, (Vestergaard and Hansen, 1984). Investigations into the feasibility of an alternative system in which the sows are not restricted have therefore been initiated. The main objective of the present study was to establish design criteria for voluntary accessed farrowing pens which will attract sows by providing them with their preferred environmental requirements.

Previous observations, not reported here, were made of the pre-farrowing behaviour of sows housed individually in large pens which contained a deep sand floor and a straw dispenser. All the sows showed nest building behaviour which culminated in the production of a recognisable nest. Typically they had the following characteristics: a shallow depression; a straw lining and a nest rim which formed a low barrier around the nest. The experiments described here were designed to establish the preferences of the peri-parturient sow for preformed lying areas which incorporated these features.

The use of malt distillers grains (draff) in ruminant diets is limited both by its moderate digestibility and variable intake. This is due to the low digestibility of its fibre and to the inhibitory effects of its unsaturated oil on rumen cellulolysis. Treatment of draff with Ca(OH)2 may ameliorate these limitations in two ways. Firstly, alkali treatment increases fibre digestibility due to hydrolysis of lignocellulosic linkages. Secondly, under alkaline conditions and in the presence of calcium ions, the unsaturated fatty acids of the draff oil form insoluble calcium soaps, thus reducing their inhibitory effects on rumen function.

Thirty Blackface-cross lambs were allocated by sex and weight to three groups of ten. The animals were penned in pairs and were offered hay ad libitum and supplemented with 350g DM/d of either mineralised draff (MD), limed draff (LD - draff treated with 80g Ca(OH)2 /kg DM, held at 60°C for 18 hours and then dried at 60°C), or a proprietary lamb finishing concentrate (C - Premium Sheep Fattening Pellets, Carrs Farm Foods, Solway Mills, Silloth). The supplements were gradually increased to 1200g DM/d over 60 days. Dry matter intakes of hay and supplements were measured daily and animals were weighed weekly. The lambs were condition scored ‘blind’ on day 60 and were assessed for slaughter.

The effect of including full-fat Colombian rice polishings, at 250 or 500 g/kg dry matter (DM) or defatted Colombian rice polishings (at 500 g/kg DM) on the fermentation of a basal diet of dried grass and medium-quality hay, in the rumen simulation technique (Rusitec), was investigated. With diets which contained 0, 0·25, 0·50 full-fat or 0·50 defatted, proportions of rice polishing, values for pH were depressed (7·22, 7·19, 7·11, 7·06 (s.e.d. 0·05)) and total volatile fatty acid concentrations were increased (52·8, 52·5, 75·5, 754 (s.e.d. 2·1) mmol/l) at the high levels of inclusion of rice polishings. Concentrations of ammonia (130, 140, 228, 209 (s.e, d. 64) mg/l) and total bacterial numbers (2·32, 2·70, 3·55, 442 (s.e.d. 0·82) × 108 per ml) were elevated by rice polishings inclusion. Numbers of cellulolytic bacteria (2·52, 1·74, 1·84, 2·10 (s.e.d. 0·60) × 106 per ml) and protozoa (19·8, 16·2, 15·8, 22·2 (s.e.d. 1·51) × 103 per ml were depressed (the latter significantly P < 0·01) by the inclusion offull-fat, but not by defatted, rice polishings.

Two feeding trails were made and in both all the diets given contained about 11 MJ metabolizable energy (ME) per kg dry matter (DM).

In trial 1, diets containing three concentrations of crude protein (CP) were given to a total of 84 Friesian bulls in each of three stages of growth. They were, in stage 1 (150 to 250 kg live weight) 130, 145 and 160 g CP per kg dietary DM; in stage 2 (250 to 350 kg live weight) 110, 130 and 145 g CP per kg dietary DM; in stage 3 (350 kg to slaughter) 95, 110 and 130 g CP per kg dietary DM. In stages 2 and 3, the source of supplementary nitrogen at each CP concentration was either soya-bean meal or non-protein nitrogen (NPN) giving six combinations of concentration and source of dietary CP. Live-weight gains in stage 1 were 1·52, 1·60 and 1·58 kg/day respectively (P > 0·05). In the stages involving both concentrations and source of nitrogen (soya-bean meal in parentheses), the mean daily live-weight gains were (1·40), 1·38, (1·32), 1·34 (1·34) and 1·31 kg for stage 2 (F > 0·05) and (1·14), 1·14, (1.24), 1·16, (1·06) and 1·08 kg for stage 3 (P < 0·05), for the diets of 130, 145 and 160 g CP per kg diet respectively.

Trial 2 consisted of six stages of growth, the first five of 45 days duration and the sixth of varying length up to a predicted slaughter weight, and seven dietary treatments. Treatment 1 was a negative control (NC) with a dietary CP concentration of 90 g/kg DM. For the other six treatments two concentrations of dietary CP were given; 120 g/kg and 140 g/kg in stages 1 and 2 and 100 g/kg or 120 g/kg in stages 3 to 6. Dietary CP concentration was increased by the addition of NPN, fish meal or soya-bean meal. A total of 98 Friesian bulls were used with an initial average live weight of 185 kg. Daily gain of the NC group in the six stages of the trial was 0·86, 0·97, 101, 1·13, 1·07 and 1·00 kg for stages 1 to 6 respectively. From the results it was concluded that feeding NPN is not efficient at live weights lower than 250 kg and that there is no advantage to feeding fish meal over soya-bean meal in diets of medium energy concentration at live weights heavier than 250 kg.

Total milk yield of sows is positively correlated with litter size (Bonsma and Oosthuizen, 1935; Wells, Beeson and Brady, 1940; Smith, 1952; Allen and Lasley, 1960) even though the amount of milk obtained by each pig decreases with increasing litter size. Positive correlations between sow milk yield and the creep feed consumption of her litter and between weaning weight and milk production have been reported by Bonsma and Oosthuizen (1935), and by Allen and Lasley (1960). Braude (1964) has comprehensively reviewed the literature on the associations between litter size, birth weight and weaning weight. Ward, Rempel and Enfield (1964) reported a positive correlation between weaning weight and post-weaning growth rate in swine.

The coat of 16 Wiltshire × Wensleydale lambs was investigated and all were woolly, with no halo hairs on the back. The bulk of the coat consisted of curly-tip fibres, and most lambs had Valley (8) or Plain (6) fibre-type arrays, only two having Ravine arrays.

There was little difference in diameter between the primary and secondary fibres in the skin, and some evidence of ‘checking’ in the central primaries. The secondary/primary follicle ratio of 4·26 compared with a mid-parent value of 4·5. There was less latticed medullation in the crossbreds than in the Wiltshire.

The adult fleeces were visually similar to those of the Wensleydale and were intermediate in weight and staple length between the parent breeds. These findings could have practical application in the improvement of ‘hair’ sheep, in that a first cross with the Wensleydale would have a fleece of medium wool lacking hair or kemp.

Six comparable groups, each of 4 castrate pigs, were used to compare the feed value of maize and sorghum as estimated by live-weight gain, food conversion efficiency, slaughter quality and ham composition.

The maize ration contained 40% yellow maize and the sorghum ration 40% Sorghum vulgare subglabrescens, all other ingredients being the same for both diets.

No statistically significant differences were found between the maize and sorghum pigs with regard to the criteria mentioned.

It is concluded that no difference exists between the feed value of the maize and the sorghum used. This result is in better agreement with the net energy values of maize and sorghum as calculated by the formula of Hoff-mann and digestion coefficients for pigs, than with “classical” starch equivalents.

The influences of age at first pregnancy and number of suckling young on maternal performance of mice were studied. Mean mating ages for early-pregnant and normal-pregnant females were 27 and 52 days respectively. Litter size from parturition (day 0) to day 12 of lactation was standardized to either 8 or 12 pups. The more rapid (P<0·01) growth rate and feed conversion efficiency of early-pregnant dams from mating to parturition continued from 0 to 12 days of lactation but occurred primarily in the first 3 days of lactation, so that there were no differences in body weight or feed intake from days 3 to 12 of lactation. There were no age at pregnancy effects on total mammary gland DNA, RNA or RNA/DNA ratio at day 12 of lactation or on individual progeny weights or feed conversion efficiency of litter growth from 3 to 12 days of lactation. Dams suckling eight young had a lower (P<0·01) feed intake, gain and feed conversion efficiency from 0 to 3 days of lactation. From 0 to 12 days of lactation, these dams were smaller, consumed less feed and their individual progeny weights were larger (P<0·01). Dams suckling eight pups had smaller (P<O01) mammary glands, less (P < 0·05) mammary gland DNA and a higher (P<0·05) RNA/DNA ratio. Interactions between pregnancy age and number suckling were not significant. Feed intake of the dam from 0 to 12 days of lactation was influenced more by litter weight gain (34%) than either dam weight gain (5%) or metabolic body size (6%). Maternal performance of mice, based on pup growth and mammary gland growth and function, was not affected by early pregnancy.

In an initial experiment, cattle receiving 5 mg. hydroxyzine dihydro-chloride daily gained 12·7% faster and manifested 12·0% better feed conversion ratio than the control, but these effects were not statistically significant under the conditions of the experiment. Carcass quality and endocrines were not affected by hydroxyzine feeding.

In formulating a genetic index for use in cow and sire evaluation, there is a need to account for all environmental factors which influence an animal's yield. To this end, milk production records are currently adjusted for, amongst other things, age at calving and month of calving. In Norway, Sweden and West Germany, records are corrected for calving interval, i.e. the number of days between subsequent calvings (Philipsson, Dommerholt, Fimland, Gaillard, Gjøl-Christensen, Lederer, McClintock and Mocquot, 1978), but in Britain no account is taken of calving interval or days dry. Analysis has shown (McClintock, 1982) that both these factors have a significant effect on yield, and hence further investigation into the value and methods of adjusting for these factors was required.

Progesterone, oestradiol-I7β and luleinizing hormone were measured by radioimmunoassay in plasma samples collected daily or on alternate days following insemination in six ‘repeat breeder’ buffaloes. The endocrine profiles in these animals demonstrated that a variety of temporary disturbances in behaviour of these circulating hormones resulted in reproductive failure.

The objective of this study was to evaluate the use of oat hulls (OH) to substitute traditional fiber (a mixture of rice hulls and mugwort (RHM) leaf) in the diets of fattening rabbits by examining on its effect on the growth performance, coefficient of total tract apparent digestibility (CTTAD) of nutrients, gastrointestinal tract development, cecum fermentation and carcass traits. A total of 160 mixed sex Hyla commercial meat rabbits (40/treatment) were used to study the effects of including OH in the diet from 30 to 80 days of age. A control diet based on RHM and containing 175.2 g crude fiber and 169.7 g CP/kg was included. Growth performance and CTTAD of nutrients were recorded from day 35 to day 80 and day 74 to day 80, respectively, whereas gastrointestinal tract development, cecum fermentation and carcass traits were determined at day 80. Increasing concentrations of OH in the diet increased average daily feed intake (P=0.0018), but have no effects on average daily gain and feed conversion ratio (P>0.05). Moreover, the 150 g/kg OH diet decreased the relative weight of full cecum and cecal content (P<0.05), but did not affect other gastrointestinal organs. The CTTAD of NDF and gross energy decreased with the inclusion of OH (P<0.05). No effects of CTTAD of ADF, ADL, dry matter, CP and ether extract were observed (P>0.05). The concentrations of total volatile fatty acid, acetic and butyric acids were greater in rabbits fed the lower levels of OH (0 to 100 g/kg) compared with higher rate (150 g/kg; P<0.05). However, the proportions of acetic, propionic and butyric acids were not affected by treatments (P>0.05). Furthermore, no significant effect on the ratios of acetic/(propionic+butyric) was observed (P>0.05). Hot carcass weight, pH (45 min, 24 h), lightness, redness, yellowness, 24-h drip loss of longissimus lumborum muscles were not affected by diet OH (P>0.05). However, dressing out percentage increased with higher (150 g/kg) inclusion of OH. It is concluded that OH can be included in rabbit diets at levels up to 100 g/kg, but negative effect on digestion and performance were observed with the increasing of OH (150 g/kg).