Determining energy utilization in growing animals enables to adjust the nutritional constraints to nutrient requirements while maximizing the ratio between lean retention and fat retention to improve feed efficiency. In turkey production, the important sexual dimorphism and differences between strains may contribute to differences in basal energy metabolism and the partitioning of energy retention between protein and lipid. The objective of this study was to determine the dynamics of energy utilization in males and females of a heavy strain of turkeys fed ad libitum from 1 to 23 weeks of age. Heat production (HP) was determined by indirect calorimetry and retained energy (RE) was calculated as the difference between metabolizable energy (ME) intake and HP. The RE as protein was determined by a nitrogen balance, while the remaining RE was assumed to be lipid. A modeling procedure allowed partitioning HP between fasting HP (FHP), activity-related HP and thermic effect of feeding. A multiple regression analysis was used to estimate the maintenance energy expenditure (MEm) and the energy efficiencies of protein and lipid retention (kp and kf, respectively). Results were expressed either per day or per kg BW0.75 per day. In comparison with females, males consumed more feed (440 v. 368 g/day), grew faster (163 v. 147 g/day) and retained more protein (38 v. 28 g/day) during the experimental period. Expressed per kg BW0.75 per day, ME intake decreased linearly with increasing age and was not affected by gender. Similarly, RE as protein decreased with increasing age and tended to be greater in males than in females, whereas RE as lipid increased with increasing age and was lower in males than in females. In addition, HP decreased with increasing age and was greater in males than in females, because of greater activity-related HP and FHP (47% and 9% greater in males compared with females). The FHP averaged 417 kJ/(kg BW)0.75 per day during the first 3 weeks of age and decreased to 317 and 277 kJ/(kg BW)0.75 per day in males and females, respectively, from 20 weeks of age onwards. Similar to FHP, MEm was lower in females than in males ((586 to 12 × BW) and (586 to 5 × BW) kJ/(kg BW)0.75 per day, respectively) and the kp and kf were estimated at 0.63 and 0.87, respectively. This study shows that the partitioning of RE and HP differs between genders in growing turkeys, which likely results in differences in nutrient requirements.

Re-esterified oils are new fat sources obtained from the chemical esterification of acid oils with glycerol (both economically interesting by-products from oil refining and biodiesel industries, respectively). The different fatty acid (FA) positional distribution and acylglycerol composition of re-esterified oils may enhance the apparent absorption of saturated fatty acids (SFA) and, therefore, their overall nutritive value, which might lead to an increased deposition of SFA. The aim of the present study was to investigate the potential use of re-esterified palm oils, in comparison with their corresponding acid and native oils in fattening pig diets, studying their effects on fatty acid apparent absorption, acylglycerol and free fatty acid (FFA) composition of feces, growth performance, carcass-fat depots and fatty acid composition of backfat. Seventy-two crossbred boars and gilts (average weight of 24.7±2.55 kg) were blocked by initial BW (nine blocks of BW for each gender), housed in adjacent individual boxes, and fed one of the four dietary treatments, which were the result of a basal diet supplemented with 4% (as-fed basis) of native palm oil (PN), acid palm oil (PA), re-esterified palm oil low in mono- and diacylglycerols (PEL), or re-esterified palm oil high in mono- and diacylglycerols (PEH). Regarding results from the digestibility balance, PA and PN showed similar apparent absorption coefficients (P>0.05), despite the high, FFA content of the former. However, re-esterified palm oils (both PEL and PEH) showed a higher apparent absorption of total FA than did their corresponding native and acid oils (P<0.001), mainly due to the increased apparent absorption of SFA (P<0.001). This resulted in a greater feed efficiency and an increased deposition of SFA in backfat of pigs fed PEH, when compared with those fed PA (P<0.05), although no differences were found for carcass-fat depots (P>0.05). We conclude that re-esterified oils are interesting fat sources to be considered in fattening pigs.

Low selection intensity due to few selection candidates available at any one time due to thinly spread year-round lambings in villages and prohibitively large nucleus requirements to provide sufficient improved rams to the production tier are the major challenges for designing effective village-based and central nucleus-based breeding programmes, respectively, for smallholder sheep farmers. To tackle these challenges, we used deterministic simulation to design three schemes in village-based programmes introducing hormonal oestrus synchronization (natural oestrus (VNE), single oestrus synchronization (VSE1) and double oestrus synchronization (VSE2)) and three schemes in central nucleus programme introducing artificial insemination (AI) (natural mating with nucleus sizes of 5% (CNM1) and 1% (CNM2) of the total ewe population and natural mating in breeding tier and AI in production tier (CAI)). The schemes were evaluated for their bio-economic and operational feasibility, taking Bonga sheep of Ethiopia as a case study. The selection intensities achieved in VNE, VSE1 and VSE2 were 2.0, 2.3 and 2.4, respectively, for selecting rams for the breeding tier and 0.0, 0.8 and 1.0, respectively, for the production tier. The profits per ewe per year from VNE, VSE1 and VSE2 were Birr 12.2, 21.7 and 24.5, but the profit from VNE for the production tier was zero. CAI generated more genetic gains in the breeding objective (Birr 4.8) than CNM1 (Birr 2.5) and CNM2 (Birr 0.0) in the production tier. However, CAI was less profitable than CNM1 and CNM2. In conclusion, hormonal oestrus synchronization was found to be a feasible technological aide to accelerate genetic progress in village-based programmes. CNM1 and CNM2 could not be recommended as CNM1 requires large nucleus of 10 325 ewes and CNM2 results in zero genetic gain in the production tier. CAI could overcome the challenge in central nucleus programmes, namely unaffordable large nucleus, but the scheme needs to be subsidized by the public sector to be economically feasible for farmers.

Genetic parameters for test-day milk flow (TDMF) of 2175 first lactations of Holstein cows were estimated using multiple-trait and repeatability models. The models included the direct additive genetic effect as a random effect and contemporary group (defined as the year and month of test) and age of cow at calving (linear and quadratic effect) as fixed effects. For the repeatability model, in addition to the effects cited, the permanent environmental effect of the animal was also included as a random effect. Variance components were estimated using the restricted maximum likelihood method in single- and multiple-trait and repeatability analyses. The heritability estimates for TDMF ranged from 0.23 (TDMF 6) to 0.32 (TDMF 2 and TDMF 4) in single-trait analysis and from 0.28 (TDMF 7 and TDMF 10) to 0.37 (TDMF 4) in multiple-trait analysis. In general, higher heritabilities were observed at the beginning of lactation until the fourth month. Heritability estimated with the repeatability model was 0.27 and the coefficient of repeatability for first lactation TDMF was 0.66. The genetic correlations were positive and ranged from 0.72 (TDMF 1 and 10) to 0.97 (TDMF 4 and 5). The results indicate that milk flow should respond satisfactorily to selection, promoting rapid genetic gains because the estimated heritabilities were moderate to high. Higher genetic gains might be obtained if selection was performed in the TDMF 4. Both the repeatability model and the multiple-trait model are adequate for the genetic evaluation of animals in terms of milk flow, but the latter provides more accurate estimates of breeding values.

The effect of including 14 g urea with either 75 g dry matter (DM) from sugar cane molasses (UM) or Colombian rice polishings (RP) at three levels, 68 (RP1), 137 (RP2) or 203 (RP3) g DM on grass hay DM intake and on rumen fermentation was investigated. An incomplete Latin-square design was used and each experimental period was divided into 12 days for adaptation to each diet followed by 9 days in metabolism cages when all measurements were made using five adult sheep.

Pooled mean values for rumen metabolites (five samples per day) were calculated. Rumen pH was not affected by the nature of the supplements. Concentrations of volatile fatty acids (VFA) (UM 92·8, RP1 84·2, RP2 86·4, and RP3 84·0 (s.e.d. 3·4) mmol/l) and lactate (UM 2·0, RP1 1/6, RP2 1/7, RP3 1/8 (s.e.d. 0·014) mmol/l) (UM v. RP, P < 0·05 and P < 0·10 respectively) were lower when RP were given, while concentration of branched and longer chain VFA (26·5, 34·0, 31·1 and 33·5 (s.e.d. 1·6) mmol/mol total VFA, UM v. RP, P < 0·01) and ammonia (98, 131, 141, 137 (s.e.d. 16·1) mg/l, UM v. RP, P < 0·05) were increased. Numbers of rumen protozoa (1·6, 3·2, 2·7, 3·3 (s.e.d. 0·75) × 105 per ml, UM v. RP, P < 0·20) tended to be higher 2 h after feeding when RP rather than UM were given. However, hay DM intake (1050, 960, 960, 880 (s.e.d. 45·3) g/day, UM v. RP, P < 0·05), and microbial protein supply (11·7, 9·3,11·1,10·8 (s.e.d. 0·59) g N per day, UM v. RP, P < 0·05), estimated from urinary purine excretion were reduced by feeding RP instead of UM.

At the levels of inclusion tested RP did not increase the efficiency of rumen fermentation and were not as effective a supplement with urea as was molasses for a low-quality forage diet.

Twenty-five Californian does were mated and given pelleted diets of either 8(LE) or 10(HE) MJ/kg dry matter (ruminant metabolizable energy values) throughout gestation and lactation. Litter sizes were adjusted to six offspring per doe. Milk yield was estimated from the weight differences of the litters before and after suckling, when the opportunity for nursing was confined to once daily. The young rabbits were allowed access to either the LE or HE diets from 18 days of age. Weaning took place at 32 days of age when four rabbits from each doe grouP × pre-weaning diet were slaughtered. Diet had no effect on litter size or birth weight, but does on the LE diet had lower live weights post partum (P < 0·05). Dry-matter consumption by the does on the LE diet was greater than for the HE diet (P < 0·05) both during gestation and lactation, but calculated metabolizable energy intakes were lower during lactation. There were no statistically significant differences between treatments for milk yield or live-weight gain to weaning and dry-matter intakes of the young at weaning, but daily live-weight gain from fostering to 18 days of age was greater for litters of does fed the HE rather than the LE diet (P < 0·05). There were no statistically significant differences between diets for carcass traits at weaning.

After weaning, 32 rabbits were given either the LE or HE diet until 2 kg live weight when they were slaughtered. A post-weaning × pre-weaning diet interaction (P < 0·05) occurred for live weights of the rabbits at the start of the post-weaning trial (i.e. after a 10-day adaptation period) with LE: LE rabbits being lighter than other rabbits as a results of poor live-weight gains during the adaptation period. Live-weight gains to slaughter were greater for the pre-weaning LE diet (P < 0·05) and the post-weaning HE diet (P < 0·01), although dry-matter intakes of the HE diet were less (P < 0·05). Rabbits on the LE diet had greater empty gut weights (P < 0·05) and lighter livers (P < 0·05) at slaughter.

An investigation of milking ability in three dairy breeds has shown that selection for superior productive performance would contribute little to the improvement of milking time. The heritability of both average rate of flow and milking time was reasonably high in all three breeds, however, and direct selection should result in effective improvements.

The problem of slow milking was more acute in the Danish Red breed, in which almost one cow in ten needed more than six minutes to milk out, than in the Black Pied or Danish Jersey breeds.

A comparison of the average rate of flow and milking time as criteria of selection for milking ability indicated that both measures were equally effective in the Black Pied and Jersey breeds, but that rate of flow was considerably more (40%) effective than milking time in the Danish Reds. This was largely because the heritability of milking time in this breed was only one-third that of milking rate.

It was concluded that the average rate of flow was to be preferred to milking time as a criterion of selection for milking ability in the Danish station testing programme.

A trial is described in which normal rations containing oleandomycin at 12½ and 25 g. per ton of meal and without the additive were given to females and castrates which had previously received creep feed with and without oxytetracycline.

A total of 48 pigs were individually fed on the rations from 50 to 200 lb. live-weight, a restricted scale of intake based on live-weight being adopted.

Between 50 and 100 lb. live-weight rate and efficiency of gain were significantly better in pigs which had not received antibiotic in the creep feed. No other treatment had any significant effect on performance.

Oleandomycin did not influence carcass measurements or dressing percentage. Minimum depth of fat at the mid-back was significantly greater in pigs which had received oxytetracycline in the creep feed while castrates had a significantly greater depth of fat at the shoulder and mid-back compared with females.

Pigs receiving the lower level of oleandomycin had significantly lighter kidneys than the control animals. Other visceral weights were not significantly influenced by the antibiotic, but females had significantly heavier kidneys, spleen and liver weights than castrates.

During winter in southern Queensland, eight rusa deer stags aged 4 years were given ad libitum lucerne (Medicago sativa) hay and confined in individual metabolism pens for 26 days. Stags ate 2·04 kg dry matter (DM) per day and drank 6·4 kg water per day, while the drinking water: food DM ratio was 3·3 l/kg. In experiment 2, seven rusa stags were given ad libitum lucerne hay or oaten (Avena spp.) hay with or without barley grain supplementation (200 g/day) for 56 days (four periods). This experiment was conducted from 26 July to 19 September 2001, when the stags were exhibiting the behaviour characteristic of the rut. Rusa stags ate 1·19 and 1·17 kg DM per day of lucerne and oaten hay respectively. Rusa stags given oaten hay drank slightly more water than those that received lucerne hay (5·34 and 4·47 kg/day, respectively). The drinking water: food DM ratios were 3·81 and 4·67 kg/kg for lucerne and oaten hay, respectively. Barley grain supplementation (200 g/day) had no influence on total food or water intakes of the rusa stags.

1. Thirty lactating British Friesian heifers and cows were individually given maize silage ad libitum containing 331 g dry matter/kg, 13 g nitrogen and 11·2 MJ of metabolizable energy per kg dry matter. Urea or aqueous ammonia was mixed in at the time of feeding to give 7·0 g nitrogen per kg silage dry matter. Urea-treated silage was given with concentrates at a high (mean 6·6 kg dry matter/day: treatment HU) or a low (mean 3·3 kg:treatment LU) level of feeding; the lower level only was given with ammonia-treated silage (treatment LA).

2. The addition of ammonia raised silage pH values from 3·9 to 4·4. Silage dry-matter intakes in lactation weeks 7 to 22 for treatments HU, LU and LA were 8·8, 11·1 and 10·7 kg/day.

3. Digestible organic matter content in the dry matter of the diets measured in vivo was not significantly affected by treatment. However, digestible organic matter intakes were significantly greater for treatment HU than for LU in weeks 7 to 10, and for LU than for LA in weeks 11 to 22.

4. The yields of milk and the contents of protein, lactose and energy did not differ between treatments. The milk yields for treatments HU, LU and LA in weeks 7 to 22 were 20·2,19·2, 18·8 kg/day respectively. Fat content of milk was significantly depressed with the HU treatment for heifers in weeks 7 to 10. The live-weight changes of the cows in weeks 7 to 22 for treatments HU, LU and LA were +0·10, −0·07 and −0·37 kg/day; the live-weight loss by cows given treatment LA was significantly greater than for LU. The live-weight change of the heifers was not affected by treatment.

The aim of this study was to compare responses to cold stress in newborn lambs and kids. Two experiments were conducted in a climatic chamber with controlled air temperatures and artificial wind and rain. The first experiment involved 25 Poll Dorset × (Border Leicester × Romney) lambs and 16 Angora × feral kids while the second involved 30 Suffolk × (Border Leicester × Romney) lambs and 26 Angora × feral kids. Rectal temperatures were measured and the rate of decline in rectal temperature estimated in both experiments. In the second experiment, metabolic rate was also measured and coat characteristics (birth coat depth and wool weight per unit area of skin) were examined. The effects of birth rank and sex on resistance to cold exposure and their interaction with species were examined in both experiments.

In experiment 1, kids had lower rectal temperatures than lambs at the start of the test but there was no species effect on the rate of decline in rectal temperature. In experiment 2, kids exhibited a significantly greater rate of decline in rectal temperature than did lambs. This difference in species effect between the experiments reflected primarily a greater rate of body cooling in the Poll Dorset-cross lambs (experiment 1) than in the Suffolk-cross lambs (experiment 2) rather than a difference between the kids in the two experiments. Additionally, in experiment 2, metabolic rate per unit live weight was significantly lower in the kids than in the lambs. No differences were apparent between the species in coat characters. Multiple-born lambs and kids were found to exhibit a significantly greater rate of decline in rectal temperature than singles, but only marginal differences in heat production were observed between the birth ranks. No significant effects of sex on resistance to cold stress were found in this study. Effects of birth rank and sex were additive with those of species. It is concluded that a combination of low heat production capacity and small body size is responsible for the generally poor responses of newborn kids to cold stress but there is probably little difference between lambs and kids in the insulative value of their birth coats.

Fleece characteristics of three distinct strains of Scottish Blackface sheep were studied by sampling eighteen ewes of the Lanark, Galloway and Lewis strains on three occasions in a single year. During the period of maximum growth the Lewis strain, which is largely of historical interest, differed significantly from the others in S/P ratio and mean weight, diameter and degree of medullation in fine and coarse fibres. The differences between the other two strains were small and although a considerable range in fleece characteristics was found, most of the whole fleeces would be classed as ‘medium’ wool.

All three strains showed a considerable reduction in follicle output during the winter and spring months when differences amongst them were minimised.

Electrophoretic variants of certain red cell esterases of rabbits (Oryctolagus cuniculus) have been shown to be under the control of two co-dominant autosomal alleles (Grunder, Sartore and Stormont, 1965). Each allele controls two zones of esterase; those controlled by EsA migrated ahead of those controlled by EsB. The heterozygote manifests not only the zones of both homozygotes but also a ‘hybrid’ zone which appears between the A and B zones.

The stomach and small intestine manifest a myoelectric activity pattern called the migrating myoelectric complex (MMC), which is controlled by both environmental and intrinsic factors. The daily MMC pattern has been little investigated, therefore the purpose of the present study was to study it, in weaned pigs given food twice a day. A wireless telemetric recording system was used for 24-h electromyography of the antrum and duodenum. The activity of the antrum showed little change if any, whereas the myoelectric activity of the duodenum significantly changed with respect to the time of day and feeding in conscious pigs. Namely, there were more frequent and regular MMC cycles occurring in the duodenum at night as compared with that during daytime. This change was due to the shortening of phase II of the MMC cycles that were registered at night. Phase I of the MMC showed transient variability in relation to feeding with no impact on the day/night differences. Phase III of the duodenal MMC cycle as well as the ‘feeding pattern’ did not change along with the time of day and feeding regime. In conclusion, daily variation in the duodenum may provide different conditions for digestive processes in the day and night.

Computer simulation was used to study the effect of assortative mating in tandem selection. Selection was applied alternatively for each of two traits of equal economic importance, the mating being assortative for the trait not subjected to selection. Two heritabilities (0·2, 0·4), two selection intensities (20/40, 20]100) and three additive genetic correlations (0·50, 0, —0·50) were considered. Assortative mating gives a response which is 1·03 to 1·10 times that achieved when random mating is employed.

Seventy-nine Scottish Blackface and 79 North × South Country Cheviot ewes were fed to increase their mean condition score by 0·5 over periods of either 4 (M/H group) or 8 (I group) weeks prior to mating. Thereafter, they were fed a live-weight maintenance ration until they were slaughtered at 50 to 65 days after mating. Reproductive tracts were recovered, corpora lutea counted and embryos counted and examined. Plasma progesterone levels were monitored at selected times after mating. The proportion of ewes in each treatment group that were pregnant at the time of slaughter was similar (0·75 to 0·83) but there was evidence of differences in the pattern of reproductive failure with treatment. The proportion of non-pregnant ewes which had apparently been pregnant initially was 0·55 in the M/H group compared with only 0·27 in the I group. Circulating progesterone levels were not affected by premating nutritional treatments but differed with breed.

Parameters of daily milk yield during the first three lactations of Chios ewes were estimated with random regression models. Data consisted of 42 675 test-day records of 7121 ewes from 75 flocks that had lambed between 1998 and 2000. Models fitted fourth order fixed regressions on Legendre polynomials of the number of days post partum and fourth order random regressions on the individual animal. (Co)variance components were estimated with Gibbs sampling. Lactations were analysed separately. The four eigen values accounted for 0·80 to 0·84, 0·11 to 0·15, 0·04 to 0·05 and about 0·01 of the animal variance, respectively, depending on lactation number. Animal variance estimates, including genetic and, partly, permanent environment effects, were high at the beginning of each lactation and decreased as lactation progressed, suggesting that the animal effect is most important to early daily records. Residual variance was highest in the middle of lactation, suggesting that non-systematic environmental factors play a bigger at that time. Animal correlation estimates between daily yield records ranged from 0·26 to 0·99, were highest for adjacent days and decreased for days further apart. The decline had a different shape in the three lactations and was more evident in the first, suggesting that the three lactations may be biologically distinct traits. Animal correlation estimates between daily and total lactation milk yield ranged from 0·61 to 0·98 and were highest in the middle and lowest towards the end of lactation. Early lactation daily yield had an animal correlation of 0·70 to 0·80 with total lactation milk yield, in all three lactations. Results of this study suggest that daily milk yield records in the early stages of lactation may be useful for selection of ewes with high producing ability and accurate prediction of total lactation milk yield.

Three groups, each of 12 West African Dwarf ewes weighing 17·5 kg at mating, had mean daily digestible organic matter intakes over the final 7 weeks of pregnancy of 249, 435 and 538 g/ewe (Treatments I, II and III respectively). Mean plasma concentrations in Treatments I, II and III were: glucose, 384, 527 and 538mg/l; non-esterified fatty acids (NEFA), 672, 441 and 427 μequiv/1; and ketones, 44·1, 30·5 and 27·3 mg/1. The differences in all metabolites between Treatment I and Treatments II and III were significant (i) <0·001). The mean birth weights of the single lambs from ewes in Treatments I to III were 1·1, 1·7 and 1·8 kg, those from ewes in Treatment I being significantly lighter than those from ewes in Treatments II and III. Lamb birth weights were significantly and positively related to energy intake and to plasma glucose concentrations, and significantly and negatively related to plasma NEFA and ketone concentrations.

The results indicate that the effect of undernourishment during late pregnancy in West African Dwarf sheep may be greater than in European breeds, and that the concentrations of blood metabolites may have a use in monitoring and regulating nutritional state in this genotype.

The effects of level of supplemental carbohydrate from turnips and level of rumen degradable nitrogen on bacterial cellulolysis, food intake, live-weight gain and diet digestibility were examined with 36 300-kg Friesian steers allocated, in a randomized-block design, to one of six dietary treatments: (i) sodium hydroxide treated straw plus a supplement of urea (30 g urea per kg straw dry matter (DM)) (uO); (ii) uO plus turnips (23·5 g DM per kg M0·75); (Hi) uO plus a high level of turnips (47 g DM per kg M0·75); (iv) (v) and (vi), the same three diets, but with additional urea (60 g urea per kg straw DM). In two separate experiments each lasting for 56 days, the turnip allowance was given in either two feeds, or one feed per day and the straw was given to appetite. Rumen fluid was obtained by stomach tube from all steers before feeding (07·30 h) and after feeding (11.00 h) on days 10 and 24 of each experiment.

Weight gains for all groups given straw plus turnips were high (> 0·90 kg/day). Increasing the quantity of turnips gave significantly increased DM intakes and weight gains but then turnips substituted for straw, and the proportion of straw consumed decreased from 0·8 to 0·6 of the dietary DM (P < 0·01) There were no significant interactions between frequency of feeding of turnips and level of urea on the variables food intake and weight gain. Fibre digestibility was not depressed by the addition of turnips to the diet. Rumen fluid pH decreased after the steers ate turnips but tended to remain above 6·7. There were no large changes in the total number of viable cellulolytic bacteria as a result of either addition of urea or turnips to the diet; counts remained in the order of 107 per ml. Steers consumed large quantities of sodium hydroxide treated straw; turnips were therefore only a small proportion of the overall DM intake with relatively little effect on bacterial cellulolysis.

Three experiments were carried out to investigate the effect of different forage types on the performance of male British Friesian calves up to 6 months of age. Unwilted and wilted silages were compared in all experiments, and in experiment 1 hay was also compared with the two silages. All the forages were compared at different levels of concentrate supplementation ranging, in different experiments, from none to ad libitum.

Calves fed wilted silage consumed 490, 90 and 200g/kg more forage dry matter respectively than those fed unwilted silage in the three experiments but this extra intake was generally not reflected in an increased rate of gain. Hay which was of lower digestibility than silage gave significantly lower live-weight gain. Quantities of silage consumed at an early age were small, with a mean daily intake over the first five weeks of 0·13 kg dry matter. Concentrate reduced silage dry-matter intake by 0·49 kg/kg concentrate dry matter consumed. Estimated mean concentrate intake required to achieve a daily gain of 0·75 kg in 1- to 6-month-old calves with silage offered ad libitum was 0·83 kg dry matter.