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Validation of fatty acid predictions in milk using mid-infrared spectrometry across cattle breeds

Published online by Cambridge University Press:  02 July 2012

M. H. T. Maurice-Van Eijndhoven ,
H. Soyeurt ,
F. Dehareng  and
M. P. L. Calus
M. H. T. Maurice-Van Eijndhoven
Affiliation:
Animal Breeding and Genomics Centre, Wageningen UR Livestock Research, PO Box 65, 8200 AB Lelystad, The Netherlands Animal Breeding and Genomics Centre, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
H. Soyeurt
Affiliation:
Animal Science Unit, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, Belgium National Fund for Scientific Research, 1000 Brussels, Belgium
F. Dehareng
Affiliation:
Valorisation of Agricultural Products, Walloon Agricultural Research Centre, 5030 Gembloux, Belgium
M. P. L. Calus
Affiliation:
Animal Breeding and Genomics Centre, Wageningen UR Livestock Research, PO Box 65, 8200 AB Lelystad, The Netherlands
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Abstract

The aim of this study was to investigate the accuracy to predict detailed fatty acid (FA) composition of bovine milk by mid-infrared spectrometry, for a cattle population that partly differed in terms of country, breed and methodology used to measure actual FA composition compared with the calibration data set. Calibration equations for predicting FA composition using mid-infrared spectrometry were developed in the European project RobustMilk and based on 1236 milk samples from multiple cattle breeds from Ireland, Scotland and the Walloon Region of Belgium. The validation data set contained 190 milk samples from cows in the Netherlands across four breeds: Dutch Friesian, Meuse-Rhine-Yssel, Groningen White Headed (GWH) and Jersey (JER). The FA measurements were performed using gas–liquid partition chromatography (GC) as the gold standard. Some FAs and groups of FAs were not considered because of differences in definition, as the capillary column of the GC was not the same as used to develop the calibration equations. Differences in performance of the calibration equations between breeds were mainly found by evaluating the standard error of validation and the average prediction error. In general, for the GWH breed the smallest differences were found between predicted and reference GC values and least variation in prediction errors, whereas for JER the largest differences were found between predicted and reference GC values and most variation in prediction errors. For the individual FAs 4:0, 6:0, 8:0, 10:0, 12:0, 14:0 and 16:0 and the groups’ saturated FAs, short-chain FAs and medium-chain FAs, predictions assessed for all breeds together were highly accurate (validation R2 > 0.80) with limited bias. For the individual FAs cis-14:1, cis-16:1 and 18:0, the calibration equations were moderately accurate (R2 in the range of 0.60 to 0.80) and for the individual FA 17:0 predictions were less accurate (R2 < 0.60) with considerable bias. FA concentrations in the validation data set of our study were generally higher than those in the calibration data. This difference in the range of FA concentrations, mainly due to breed differences in our study, can cause lower accuracy. In conclusion, the RobustMilk calibration equations can be used to predict most FAs in milk from the four breeds in the Netherlands with only a minor loss of accuracy.

Keywords

milkfatty acidmid-infrared spectrometrycattle breeds
Type
Product quality, human health and well-being
Information
animal , Volume 7 , Issue 2 , February 2013 , pp. 348 - 354
Copyright
Copyright © The Animal Consortium 2012

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