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Timed artificial insemination plus heat I: effect of estrus expression scores on pregnancy of cows subjected to progesterone–estradiol-based protocols

Published online by Cambridge University Press:  15 April 2019

E. Nogueira Open the ORCID record for E. Nogueira [Opens in a new window] ,
M. R. Silva ,
J. C. B. Silva ,
U. P. G. Abreu ,
N. A. Anache ,
K. C. Silva ,
C. J. T. Cardoso ,
P. Sutovsky  and
W. B. Rodrigues
E. Nogueira*
Affiliation:
Embrapa Pantanal, Rua 21 de Setembro 1880, Corumbá, MS 79320-900, Brasil Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443, Campo Grande -MS, 79074-460, Brasil
M. R. Silva
Affiliation:
Melhore Animal Consultoria LTDA, Rua Glycia Pedroso Fenerich, 147 box 06, Jaboticabal- SP, 14871-835, Brasil
J. C. B. Silva
Affiliation:
Embrapa Pantanal, Rua 21 de Setembro 1880, Corumbá, MS 79320-900, Brasil
U. P. G. Abreu
Affiliation:
Embrapa Pantanal, Rua 21 de Setembro 1880, Corumbá, MS 79320-900, Brasil
N. A. Anache
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443, Campo Grande -MS, 79074-460, Brasil
K. C. Silva
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443, Campo Grande -MS, 79074-460, Brasil
C. J. T. Cardoso
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443, Campo Grande -MS, 79074-460, Brasil
P. Sutovsky
Affiliation:
Department of Obstetrics, Gynecology & Women’s Health, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
W. B. Rodrigues
Affiliation:
Melhore Animal Consultoria LTDA, Rua Glycia Pedroso Fenerich, 147 box 06, Jaboticabal- SP, 14871-835, Brasil
*
†E-mail: [email protected]
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Abstract

Expression of estrus near timed artificial insemination (TAI) is associated with greater fertility, and estrus detection could improve TAI fertility or direct TAI management, although accurate estrus detection can be difficult and time-consuming using traditional methods. The aim of this study is to evaluate influence of estrus on pregnancy (artificial insemination pregnancy rates (P/AI)) and to validate an alternative method to classify estrus/heat expression using tail chalking (HEATSC) in postpartum Bos indicus cows subjected to TAI in progesterone–estrogen-based protocols. In experiment 1 (Exp. 1), cows (5491) were subjected to visual observation of estrus after progesterone device removal, before TAI, and P/AI was evaluated according to estrus and body condition score (BCS). Cows received a progesterone device and 2 mg estradiol benzoate (EB). After 8 days, the device was removed and 150 μg of d-cloprostenol and 300 IU equine chorionic gonadotrophin was given. Later, animals in Exp. 1 received 1 mg EB and TAI 44 to 48 h. In the Exp. 2 – 3830 cows using similar protocol, received different ovulation inducers: 1 mg EB (n=1624) or 1 mg estradiol cypionate (EC; n=2206) on day 8 (D8). Cows were then marked with chalk, and HEATSC evaluated at TAI on D10 (HEATSC1 – no chalk removal=no estrus expression; HEATSC2 – partial chalk removal=low estrus expression; HEATSC3 – near complete/complete chalk removal=high estrus expression). In Exp. 1, cows showing estrus presented greater P/AI (48.4% v. 40.2%, P<0.05). In Exp. 2, P/AI (HEATSC1 – 40.0%; HEATSC2 – 49.7%; HEATSC3 – 60.9%; P<0.001), and larger follicle timed artificial insemination (LFTAI) (<0.001) varied according to HEATSC. There was no difference in P/AI (P=0.41) or LFTAI (P=0.33) according to ovulation inducer. Cows with greater BCS showed greater P/AI in both experiments (P<0.05). Estrus presence and greater HEATSC improved P/AI, and EC v. EB used promoted differential estrus manifestation (cows showing HEATSC2 and HEATSC3: 79.5% with EB v. 69.98% with EC use, P<0.001), however, with similar P/AI. The use of HEATSC in B. indicus cows subjected to TAI is useful to identify cows with greater estrus expression and consequently improved pregnancy rates in TAI, allowing the cows with low HEATSC to be targeted for additional treatments aimed at improving P/AI.

Keywords

Bos indicusartificial inseminationfolliclereproductionheat score
Type
Research Article
Information
animal , Volume 13 , Issue 10 , October 2019 , pp. 2305 - 2312
Copyright
© The Animal Consortium 2019 

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References

ASSOCIAÇÃO BRASILEIRA DE INSEMINAÇÃO ARTIFICIAL 2015. Índex ASBIA- importação, exportação e comercialização de sêmen, 4th edition. ASBIA, São Paulo, SP, Brazil.Google Scholar
Ayres, H, Martins, CM, Ferreira, RM, Mello, JE, Dominguez, JH, Souza, AH, Valentin, R, Santos, IC and Baruselli, PS 2008. Effect of timing of estradiol benzoate administration upon synchronization of ovulation in suckling Nelore cows (Bos indicus) treated with a progesterone-releasing intravaginal device. Animal Reproduction Science 109, 77–87.CrossRefGoogle ScholarPubMed
Baruselli, P, Sales, J, Sala, R and Vieira, L 2012. History, evolution and perspectives of timed artificial insemination programs in Brazil. Animal Reproduction 9, 139–152.Google Scholar
Baruselli, PS, Reis, EL, Marques, MO, Nasser, LF and Bo, GA 2004. The use of hormonal treatments to improve reproductive performance of anestrous beef cattle in tropical climates. Animal Reproduction Science 82–83, 479–486.CrossRefGoogle ScholarPubMed
Batista, DSN, Abreu, UGP, Ferraz Filho, PB and Rosa, AN 2012. Índices reprodutivos do rebanho Nelore da fazenda Nhumirim, Pantanal da Nhecolândia. Acta Scientiarum. Animal Sciences 34, 71–76.Google Scholar
Binelli, M, Thatcher, WW, Mattos, R and Baruselli, PS 2001. Antiluteolytic strategies to improve fertility in cattle. Theriogenology 56, 1451–1463.CrossRefGoogle ScholarPubMed
Bó, GA, Tríbulo, H, Caccia, M and Tríbulo, R 2001. Pregnancy rates in embryo recipients treated with progesterone vaginal devices and transferred without estrus detection. Theriogenology 55, 357. (abstract).Google Scholar
Buhi, WC 2002. Characterization and biological roles of oviduct-specific, oestrogen-dependent glycoprotein. Reproduction 123, 355–362.CrossRefGoogle ScholarPubMed
Burton, MJ, Dziuk, HE, Fahning, ML and Zemjanis, R 1990. Effects of oestradiol cypionate on spontaneous and oxytocin-stimulated postpartum myometrial activity in the cow. British Veterinary Journal 146, 309–315.CrossRefGoogle ScholarPubMed
Busch, DC, Atkins, JA, Bader, JF, Schafer, DJ, Patterson, DJ, Geary, TW and Smith, MF 2008. Effect of ovulatory follicle size and expression of estrus on progesterone secretion in beef cows. Journal Animal Science 86, 553–563.CrossRefGoogle ScholarPubMed
Carter, F, Forde, N, Duffy, P, Wade, M, Fair, T, Crowe, MA, Evans, AC, Kenny, DA, Roche, JF and Lonergan, P 2008. Effect of increasing progesterone concentration from day 3 of pregnancy on subsequent embryo survival and development in beef heifers. Reproduction Fertility and Development 20, 368–375.CrossRefGoogle ScholarPubMed
Clemente, M, de La Fuente, J, Fair, T, Al Naib, A, Gutierrez-Adan, A, Roche, JF, Rizos, D and Lonergan, P 2009. Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium? Reproduction 138, 507–517.CrossRefGoogle ScholarPubMed
Gentry, GT Jr, Walker, RS and Gentry, LR 2016. Impacts of incorporation of follicle stimulating hormone into an estrous synchronization protocol for timed artificial insemination of crossbred beef cattle. Animal Reproduction Science 168, 19–25.CrossRefGoogle ScholarPubMed
Hawk, HW and Cooper, BS 1975. Improvement of sperm transport by the administration of estradiol to estrous ewes. Journal Animal Science 41, 1400–1406.CrossRefGoogle ScholarPubMed
Hillegass, J, Lima, FS, Sa Filho, MF and Santos, JE 2008. Effect of time of artificial insemination and supplemental estradiol on reproduction of lactating dairy cows. Journal Dairy Science 91, 4226–4237.CrossRefGoogle ScholarPubMed
Jinks, EM, Smith, MF, Atkins, JA, Pohler, KG, Perry, GA, Macneil, MD, Roberts, AJ, Waterman, RC, Alexander, LJ and Geary, TW 2013. Preovulatory estradiol and the establishment and maintenance of pregnancy in suckled beef cows. Journal Animal Science 91, 1176–1185.10.2527/jas.2012-5611CrossRefGoogle ScholarPubMed
Kasimanickam, R, Cornwell, JM and Nebel, RL 2005. Fertility following fixed-time AI or insemination at observed estrus in Ovsynch and Heatsynch programs in lactating dairy cows. Theriogenology 63, 2550–2559.CrossRefGoogle ScholarPubMed
Kastelic, JP, Pierson, RA and Ginther, OJ 1990. Ultrasonic morphology of corpora lutea and central luteal cavities during the estrous cycle and early pregnancy in heifers. Theriogenology 34, 487–498.CrossRefGoogle ScholarPubMed
Mann, GE, Lamming, GE, Robinson, RS and Wathes, DC 1999. The regulation of interferon-tau production and uterine hormone receptors during early pregnancy. Journal Reproduction Fertility suppl. 54, 317-328.Google ScholarPubMed
Meneghetti, M, Sa Filho, OG, Peres, RF, Lamb, GC and Vasconcelos, JL 2009. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows I: basis for development of protocols. Theriogenology 72, 179–189.CrossRefGoogle ScholarPubMed
Pereira, MHC, Wiltbank, MC and Vasconcelos, JLM 2016. Expression of estrus improves fertility and decreases pregnancy losses in lactating dairy cows that receive artificial insemination or embryo transfer. Journal Dairy Science 99, 2237–2247.CrossRefGoogle ScholarPubMed
Perry, GA and Perry, BL 2008. Effect of preovulatory concentrations of estradiol and initiation of standing estrus on uterine pH in beef cows. Domestic Animal Endocrinology 34, 333–338.CrossRefGoogle ScholarPubMed
Perry, GA, Smith, MF, Lucy, MC, Green, JA, Parks, TE, MacNeil, MD, Roberts, AJ and Geary, TW 2005. Relationship between follicle size at insemination and pregnancy success. Proceedings of the National Academy of Sciences 102, 5268–5273.CrossRefGoogle ScholarPubMed
Richardson, BN, Hill, SL, Stevenson, JS, Djira, GD and Perry, GA 2016. Expression of estrus before fixed-time AI affects conception rates and factors that impact expression of estrus and the repeatability of expression of estrus in sequential breeding seasons. Animal Reproduction Science 166, 133–140.CrossRefGoogle ScholarPubMed
Ryan, DP, Prichard, JF, Kopel, E and Godke, RA 1993. Comparing early embryo mortality in dairy cows during hot and cool seasons of the year. Theriogenology 39, 719–737.CrossRefGoogle Scholar
Sa Filho, MF, Santos, JE, Ferreira, RM, Sales, JN and Baruselli, PS 2011. Importance of estrus on pregnancy per insemination in suckled Bos indicus cows submitted to estradiol/progesterone-based timed insemination protocols. Theriogenology 76, 455–463.CrossRefGoogle ScholarPubMed
Sa Filho, OG, Meneghetti, M, Peres, RF, Lamb, GC and Vasconcelos, JL 2009. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows II: strategies and factors affecting fertility. Theriogenology 72, 210–218.CrossRefGoogle ScholarPubMed
Sales, JN, Carvalho, JB, Crepaldi, GA, Cipriano, RS, Jacomini, JO, Maio, JR, Souza, JC, Nogueira, GP and Baruselli, PS 2012. Effects of two estradiol esters (benzoate and cypionate) on the induction of synchronized ovulations in Bos indicus cows submitted to a timed artificial insemination protocol. Theriogenology 78, 510–516.CrossRefGoogle ScholarPubMed
Spell, AR, Beal, WE, Corah, LR and Lamb, GC 2001. Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle. Theriogenology 56, 287–297.CrossRefGoogle ScholarPubMed
Torres, JR Jr, Penteado, L, Sales, JN, Sa Filho, MF, Ayres, H and Baruselli, PS 2014. A comparison of two different esters of estradiol for the induction of ovulation in an estradiol plus progestin-based timed artificial insemination protocol for suckled Bos indicus beef cows. Animal Reproduction Science 151, 9–14.CrossRefGoogle Scholar
Uslenghi, G, Chaves, SG, Cabodevila, J and Callejas, S 2014. Effect of estradiol cypionate and amount of progesterone in the intravaginal device on synchronization of estrus, ovulation and on pregnancy rate in beef cows treated with FTAI based protocols. Animal Reproduction Science 145, 1–7.CrossRefGoogle ScholarPubMed
Vynckier, L, Debackere, M, De Kruif, A and Coryn, M 1990. Plasma estradiol-17 beta concentrations in the cow during induced estrus and after injection of estradiol-17 beta benzoate and estradiol-17 beta cypionate--a preliminary study. Journal of Veterinary Pharmacology and Therapeutics 13, 36–42.CrossRefGoogle ScholarPubMed
Wiltbank, MC and Pursley, JR 2014. The cow as an induced ovulator: timed AI after synchronization of ovulation. Theriogenology 81, 170–185.CrossRefGoogle ScholarPubMed