Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-05T05:03:34.716Z Has data issue: false hasContentIssue false
  • English
  • Français

Stable isotopes to study sulfur amino acid utilization in broilers

Published online by Cambridge University Press:  10 June 2020

R. M. Suzuki Open the ORCID record for R. M. Suzuki [Opens in a new window] ,
L. G. Pacheco Open the ORCID record for L. G. Pacheco [Opens in a new window] ,
J. C. P. Dorigam Open the ORCID record for J. C. P. Dorigam [Opens in a new window] ,
J. C. Denadai Open the ORCID record for J. C. Denadai [Opens in a new window] ,
G. S. Viana Open the ORCID record for G. S. Viana [Opens in a new window] ,
H. R. Varella ,
C. C. N. Nascimento Open the ORCID record for C. C. N. Nascimento [Opens in a new window] ,
J. Van Milgen Open the ORCID record for J. Van Milgen [Opens in a new window]  and
N. K. Sakomura Open the ORCID record for N. K. Sakomura [Opens in a new window]
R. M. Suzuki
Affiliation:
Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil
L. G. Pacheco
Affiliation:
Department of Veterinary Clinic and Surgery, São Paulo State University, Jaboticabal, 148830-900, Brazil
J. C. P. Dorigam
Affiliation:
Evonik Nutrition & Care GmbH, Hanau, 63457, Germany
J. C. Denadai
Affiliation:
Stable Isotope Center, São Paulo State University, Botucatu, 18618-689, Brazil
G. S. Viana
Affiliation:
Natural Resources Institute Finland, Luke, Jokioinen, 31600, Finland
H. R. Varella
Affiliation:
Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil
C. C. N. Nascimento
Affiliation:
Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil
J. Van Milgen
Affiliation:
UMR1348 PEGASE, INRA, Agrocampus Ouest, Saint-Gilles, 35590, France
N. K. Sakomura*
Affiliation:
Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil
*
†E-mail: [email protected]
Get access

Abstract

Nutritionists have been discussing whether the dietary supplementation of cyst(e)ine is required as a part of the dietary methionine (Met) in the total sulfur amino acid (TSAA) requirement to achieve optimum performance in broilers. Part of Met is converted to cysteine (Cys) to meet the Cys requirement, especially for feather growth. The TSAA requirement has been determined by using graded levels of free Met in the diet, without supplementation of free cyst(e)ine. It has also been argued that the Met to Cys ratio (Met : Cys) changes with age and even with different Met sources. The objective of this study was to evaluate the two sources of Met, while determining the proportion of Met and Cys in total dietary TSAA that optimize the performance of broilers. A performance assay was carried out in a factorial arrangement (5 × 2) using 1080 broilers from 42 to 56 days of age fed diets having different dietary proportions of Met and Cys (44 : 56, 46 : 54, 48 : 52, 50 : 50 or 52 : 48) while maintaining the same dietary TSAA in the diets. Two synthetic Met sources (dl-Met or l-Met) were used for each of the diets with different dietary Met : Cys ratios. Twenty-one broilers of the same age were fed the diets 44 : 56, 48 : 52 and 52 : 48 by supplementing the diet with L-(15N) Met or L-(15N2) Cystine to study the metabolism of TSAA. No differences were observed between Met sources for feed intake, BW gain and feed conversion ratio (FCR; P > 0.05); however, FCR was numerically improved at 50 : 50 Met : Cys. Regarding TSAA utilization, the conversion of Met to Cys increased with increase in Met : Cys ratios, but the concentration of Met intermediates decreased. Broiler chickens responded to different dietary proportions of sulfur amino acids by altering their sulfur amino acid metabolism, and diets containing 50 : 50 Met : Cys is recommended for broilers of age 42 to 56 days.

Keywords

broilercysteinelabeled amino acidmethioninetranssulfuration
Type
Research Article
Information
animal , Volume 14 , Issue S2: 9th Workshop on Modelling Nutrient Digestion and Utilization in Farm Animals (MODNUT) , August 2020 , pp. s286 - s293
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of The Animal Consortium

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

a

Present address: Department of Animal Science, São Paulo State University, Poultry Science Laboratory, Lavinesp, Via de Acesso Professor Paulo Donato Castelane Castellane s/n 14883-900, Jaboticabal, Brazil

References

Association of Official Analytical Chemists (AOAC) 1997. In Official methods of analysis, 16th edition/3rd revision edition (ed. Cunniff, P). AOAC International, Gaithersburg, MD, USA.Google Scholar
Baker, DH 2006. Comparative species utilization and toxicity of sulfur amino acids. The Journal of Nutrition 136, 1670S–1675S.10.1093/jn/136.6.1670SCrossRefGoogle ScholarPubMed
Baker, DH, Fernandez, SR, Webel, DM and Parsons, CM 1996. Sulfur amino acid requirement and cystine replacement value of broiler broilers during the period three to six weeks posthatching. Poultry Science 75, 737–742.10.3382/ps.0750737CrossRefGoogle Scholar
Baker, DH and Han, Y 1993. Bioavailable level and source of cysteine determine protein quality of a commercial enteral product: adequacy of tryptophan but deficiency of cysteine for rats fed enteral product prepared fresh or stored beyond shelf life. The Journal of Nutrition 123, 541–546.10.1093/jn/123.3.541CrossRefGoogle ScholarPubMed
Chung, TK and Baker, DH 1992. Maximal portion of the young pig’s sulfur amino acid requirement that can be furnished by cystine. Journal of Animal Science 70, 1182–1187.10.2527/1992.7041182xCrossRefGoogle Scholar
Cobb-Vantress 2012. Cobb 500 broiler management guide. Retrieved on 20 April 2020 from http://www.tt-trade.cz/docs/cobb-broiler-en.pdfGoogle Scholar
Dibner, JJ and Ivey, FJ 1992. Capacity in the liver of the broiler chick for conversion of supplemental methionine activity to L-methionine. Poultry Science 71, 700–708.10.3382/ps.0710700CrossRefGoogle ScholarPubMed
Fontaine, J 2003. Amino acid analysis of feeds. In Amino acids in animal nutrition, 2nd edition (ed. D’Mello, JPF), pp. 15–40. CABI Publishing, Wallingford, UK.10.1079/9780851996547.0015CrossRefGoogle Scholar
Fuller, MF, McWilliam, R, Wang, TC and Giles, LR 1989. The optimum dietary amino acid pattern for growing pigs: 2. Requirements for maintenance and for tissue protein accretion. British Journal of Nutrition 62, 255–267.10.1079/BJN19890028CrossRefGoogle ScholarPubMed
Graber, G and Baker, DH 1971. Sulfur amino acid nutrition of the growing chick: quantitative aspects concerning the efficacy of dietary methionine, cysteine and cystine. Journal of Animal Science 33, 1005–1011.10.2527/jas1971.3351005xCrossRefGoogle ScholarPubMed
Hartsook, EW and Mitchell, HH 1956. The effect of age on the protein and methionine requirements of the rat. Journal of Nutrition 60, 173–195.10.1093/jn/60.2.173CrossRefGoogle ScholarPubMed
Hasegawa, H, Shinohara, Y, Akahane, K and Hashimoto, T 2005. Direct detection and evaluation of conversion of D-methionine into L-methionine in rats by stable isotope methodology. The Journal of Nutrition 135, 2001–2005.10.1093/jn/135.8.2001CrossRefGoogle ScholarPubMed
Heger, J, Phung, TV and Křížová, L 2002. Efficiency of amino acid utilization in the growing pig at suboptimal levels of intake: lysine, threonine, sulfur amino acids and tryptophan. Journal of Animal Physiology and Animal Nutrition 86, 153–165.10.1046/j.1439-0396.2002.00368.xCrossRefGoogle ScholarPubMed
Huyghebaert, G and Pack, M 1996. Effects of dietary protein content, addition of nonessential amino acids and dietary methionine to cysteine balance on responses to dietary sulfur-containing amino acids in broilers. British Poultry Science 37, 623–639.10.1080/00071669608417892CrossRefGoogle Scholar
Kalinowski, AE, Moran, T Jr and Wyatt, CL 2003. Methionine and cystine requirements of slow- and fast-feathering broiler males from three to six weeks of age. Poultry Science 82, 1428–1437.10.1093/ps/82.9.1428CrossRefGoogle ScholarPubMed
Le Floc’h, N, Melchior, D and Obled, C 2004. Modifications of protein and amino acid metabolism during inflammation and imunne system activation. Livestock Production Science 87, 37–45.10.1016/j.livprodsci.2003.09.005CrossRefGoogle Scholar
Llames, C and Fontaine, J 1994. Determination of amino acids in feeds: collaborative study. Journal of AOAC International 77, 1362–1402.10.1093/jaoac/77.6.1362CrossRefGoogle Scholar
López, S, France, J, Gerrits, WJ, Dhanoa, MS, Humphries, DJ and Dijkstra, J 2000. A generalized Michaelis-menten equation for the analysis of growth. Journal of Animal Science 78, 1816–1828.10.2527/2000.7871816xCrossRefGoogle Scholar
Muramatsu, T, Hiramoto, K, Tasaki, I and Okomura, J 1987. Effect of protein starvation on protein turnover in liver, oviduct and whole body of laying hens. Comparative Biochemistry Physiology Part B 87, 227–232.10.1016/0305-0491(87)90134-9CrossRefGoogle ScholarPubMed
National Council Research (NRC) 1994. Nutrient requirements of poultry, 9th revised edition. National Academy Press, Washington, DC, USA.Google Scholar
Pacheco, LG, Sakomura, NK, Suzuki, RM, Dorigam, JCP, Viana, GS, Van Milgen, J and Denadai, JC 2018. Methionine to cystine ratio in the total sulfur amino acid requirements and sulfur amino acid metabolism using labelled amino acid approach for broilers. BMC Veterinary Research 14, 1–11.10.1186/s12917-018-1677-8CrossRefGoogle ScholarPubMed
Rostagno, HS, Albino, LFT, Hannas, MI, Donzele, JL, Sakomura, NK, Perazzo, FG, Saraiva, A, Abreu, MLT De, Rodrigues, PB, Oliveira, RF De, Barreto, SLT and Brito, CO 2017. Brazilian tables for poultry and swine: composition of feedstuffs and nutritional requirements, 4th edition. Departamento de Zootecnia - UFV, Viçosa, BR.Google Scholar
Sakomura, NK and Rostagno, HS 2016. Métodos de Pesquisa em Nutrição de Monogástricos, 2nd edition. Funep, Jaboticabal, Brazil.Google Scholar
Schutte, JB and Pack, M 1995. Sulfur amino acid requirement of broiler chicks from fourteen to thirty-eight days of age. 1. Performance and carcass yield. Poultry Science 74, 480–487.10.3382/ps.0740480CrossRefGoogle ScholarPubMed
Selhub, J and Miller, JW 1992. The pathogenesis of homocysteinemia: interruption of the coordinate regulation by S-adenosylmethionine of the remethylation and transsulfuration of homocysteine. American Journal of Clinical Nutrition 55, 131–138.10.1093/ajcn/55.1.131CrossRefGoogle ScholarPubMed
Selhub, J 1999. Homocysteine metabolism. Annual Review of Nutrition 19, 217–246.10.1146/annurev.nutr.19.1.217CrossRefGoogle ScholarPubMed
Shannon, BM, Howe, JM and Clark, HE 1972. Interrelationships between dietary methionine and cystine as reflected by growth, certain hepatic enzymes and liver composition of weanling rats. The Journal of Nutrition 102, 557–562.10.1093/jn/102.4.557CrossRefGoogle ScholarPubMed
Suzuki, RM, Sakomura, NK, Pacheco, LG, Dorigam, JCP, Ribeiro, HR, Viana, GS, Oliveira, MJK and Leme, BB 2019. Optimum proportion of sulfur amino acids for broiler chickens. Advances in Animal Biosciences 10, 327.Google Scholar
Tesseraud, S, Coustard, SM, Collin, A and Seiliez, I 2009. Role of sulfur amino acids in controlling nutrient metabolism and cell functions: implications for nutrition. British Journal of Nutrition 101, 1132–1139.10.1017/S0007114508159025CrossRefGoogle Scholar
Trivelin, PCO, Victoria, RLand Rodrigues, JCS 1995. Aproveitamento por soqueira de cana-de-açúcar de final de safra do nitrogênio da aquamônia-15N aplicado ao solo em complemento à vinhaça. Pesquisa Agropecuária Brasileira 30, 1375–1385.Google Scholar
Wheeler, KB and Latshaw, JD 1981. Sulfur amino acid requirements and interactions in broilers during two growth periods. Poultry Science 60, 228–236.10.3382/ps.0600228CrossRefGoogle ScholarPubMed
Yarnes, CT and Herszage, J 2017 The relative influence of derivatization and normalization procedures on the compound-specific stable isotope analysis of nitrogen in amino acids. Rapid Communication Mass Spectrometer 3, 693–704.10.1002/rcm.7832CrossRefGoogle Scholar
Supplementary material: File

Suzuki et al. supplementary material

Suzuki et al. supplementary materials

Download Suzuki et al. supplementary material(File)
File 21.9 KB