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SNPs detected in the yak MC4R gene and their association with growth traits

Published online by Cambridge University Press:  11 March 2015

X. Cai ,
T. D. Mipam ,
F. F. Zhao  and
L. Sun
X. Cai*
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
T. D. Mipam
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
F. F. Zhao
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
L. Sun
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
*
†E-mail: [email protected]
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Abstract

MC4R (melanocortin 4 receptor) is expressed in the appetite-regulating areas of the brain and takes part in leptin signaling pathways. Sequencing of the coding region of the MC4R gene for 354 yaks identified the following five single nucleotide polymorphisms (SNPs): SNP1 (273C>T), SNP2 (321 G>T), SNP3 (864 C>A), SNP4 (1069G>C) and SNP5 (1206 G>C). SNP1, SNP2 and SNP3 were synonymous mutations, whereas SNP4 and SNP5 were missense mutations resulting in amino acid substitutions (V286L and R331S). Pairwise linkage disequilibrium (LD) analysis indicated that two pairs of SNPs, SNP2 and SNP5 (r2=0.81027) and SNP4 and SNP5 (r2=0.53816), exhibited higher degrees of LD. CC genotype of SNP4, CGACG and CTCCC haplotypes for all SNPs were associated with increased BW of animals that were 18 months old and with the average daily gain. The secondary structure and transmembrane region prediction of the yak MC4R protein suggested that SNP4 was correlated with influential changes in the seventh transmembrane domain of the MC4R protein and with the functional deterioration or even incapacitation of MC4R, which may contribute to the increased feed intake, BW and average daily gain of the yaks with CC genotypes. The data from this study suggested that 1069G>C SNP of the MC4R gene could be used in marker-assisted selection of growth traits in the Maiwa yak breed.

Keywords

Maiwa yakMC4R geneSNPsgrowth traits
Type
Research Article
Information
animal , Volume 9 , Issue 7 , July 2015 , pp. 1097 - 1103
Copyright
© The Animal Consortium 2015 

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Footnotes

*

These are the first co-authors.

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