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Serum cortisol and haematological, biochemical and antioxidant enzyme variables in horse blood sampled in a slaughterhouse lairage, immediately before stunning and during exsanguination

Published online by Cambridge University Press:  31 January 2012

A. Nemec Svete ,
N. Čebulj-Kadunc ,
R. Frangež  and
P. Kruljc
A. Nemec Svete*
Affiliation:
Clinic for Surgery and Small Animal Medicine, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
N. Čebulj-Kadunc
Affiliation:
Institute for Physiology, Pharmacology and Toxicology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
R. Frangež
Affiliation:
Institute for Physiology, Pharmacology and Toxicology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
P. Kruljc
Affiliation:
Clinic for Reproduction and Horses, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
*
†E-mail: [email protected]
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Abstract

The aim of the study was to determine changes of serum cortisol and biochemical, haematological and antioxidant enzyme variables in the blood of horses sampled during the pre-slaughter period (in the lairage and in the stunning box) and during exsanguination. A total of 24 Slovenian warm-blooded horses were observed. Blood samples for determination of serum cortisol and biochemical, haematological (red blood cell count, haematocrit, haemaglobin concentration) and antioxidant enzyme (whole blood superoxide dismutase and glutathione peroxidase) variables were collected by venipuncture of the left jugular vein in the lairage pen, 60 min before stunning (lairage) and immediately before stunning (stunning box). At exsanguination, blood samples were collected from the wound at the time of jugular vein sticking. During blood collection in the lairage pen and in the stunning box, horses were gently restrained with a halter. They were stunned using a penetrating captive bolt pistol impelled by air and were bled by jugular vein sticking. Horses were physically active in the lairage pen and in the race before entering the stunning box. After stunning, the horses showed paddling movements with their legs. In horses, the plasma lactate and glucose concentrations, the serum potassium concentration, the activities of the serum muscle enzymes aspartate aminotransferase and creatine kinase, and values of most of the other biochemical (Table 1) and haematological variables (Table 2), were significantly (P < 0.05) higher at exsanguination, than in blood sampled while they were in the lairage and in the stunning box. The serum concentrations of cortisol and chloride and the activities of alanine aminotransferase and antioxidant enzymes were not significantly different between the pre-slaughter period and exsanguination. All selected blood variables were not significantly different between the lairge and the stunning box sampling time, indicating no physiological stress responses of the investigated horses to stressors, such as novelty of the pre-slaughter environment and handling, present in the slaughterhouse between the lairage and the stunning box. However, the significantly higher values, at exsanguination, for the plasma lactate and glucose concentrations, serum muscle enzyme activities and haematological variables, than during the pre-slaughter period, might partially be attributed to stimulation of the sympathetic nervous system, caused by stunning and bleeding.

Keywords

horsesslaughterstressantioxidant enzymesblood chemistry
Type
Full Paper
Information
animal , Volume 6 , Issue 8 , August 2012 , pp. 1300 - 1306
Copyright
Copyright © The Animal Consortium 2012

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References

Alexander, SL, Irvine, CHG 1998. The effect of social stress on adrenal axis activity in horses: the importance of monitoring corticosteroid-binding globulin capacity. Journal of Endocrinology 157, 425–432.CrossRefGoogle ScholarPubMed
Boissy, A 1995. Fear and fearfulness in animals. The Quarterly Review of Biology 70, 165–191.CrossRefGoogle ScholarPubMed
Cockram, MS, Corley, KTT 1991. Effect of pre-slaughter handling on the behaviour and blood composition of beef cattle. British Veterinary Journal 147, 444–454.CrossRefGoogle ScholarPubMed
Colahan, PT, Merritt, AM, Moore, JN, Mayhew, IGJ 1999. Equine medicine and surgery, 5th edition. Mosby, St Louis, MO, USA.Google Scholar
Deaton, CM, Marlin, DJ 2003. Exercise-associated oxidative stress. Clinical Techniques in Equine Practice 2, 278–291.CrossRefGoogle Scholar
Dotan, Y, Lichtenberg, D, Pinchuk, I 2004. Lipid peroxidation cannot be used as a universal criterion of oxidative stress. Progress in Lipid Research 43, 200–227.CrossRefGoogle ScholarPubMed
Eades, SC, Bounous, DI 1997. Significance of laboratory tests. In Laboratory profiles of equine diseases, 1st edition (ed. PW Pratt), pp. 1–31. Mosby, St Louis, MO, USA.Google Scholar
Fazio, E, Medica, P, Aronica, V, Grasso, L, Ferlazzo, A 2008. Circulating β-endorphin, adrenocorticotrophic hormone and cortisol levels of stallions before and after short road transport: stress effects of different distances. Acta Veterinaria Scandinavica, 50, 6. Published online by BioMed Central 3 March 2008.CrossRefGoogle Scholar
Frank, JJ, Bermes, EW, Bickel, MJ, Watkins, BF 1978. Effect of in vitro hemolysis on chemical values for serum. Clinical Chemistry 24, 1966–1970.CrossRefGoogle ScholarPubMed
Gorecka, R, Sitarska, E, Klucinski, W 2002. Antioxidant parameters of horses according to age, sex, breed and environment. Polish Journal of Veterinary Sciences 5, 209–216.Google ScholarPubMed
Grandin, T 1997. Assessment of stress during handling and transport. Journal of Animal Science 75, 249–257.CrossRefGoogle ScholarPubMed
Hada, T, Onaka, T, Kusunose, R, Yagi, K 2001. Effects of novel environmental stimuli on neuroendocrine activity in thoroughbred horses. Journal of Equine Science 12, 33–38.CrossRefGoogle Scholar
Hambrecht, E, Eissen, JJ, Newman, DJ, Smits, CHM, den Hartog, LA, Verstegen, MWA 2005. Negative effects of stress immediately before slaughter on pork quality are aggravated by suboptimal transport and lairage conditions. Journal of Animal Science 83, 440–448.CrossRefGoogle ScholarPubMed
Harbuz, MS, Lightman, SL 1992. Stress and the hypothalamo–pituitary–adrenal axis: acute, chronic and immunological activation. Journal of Endocrinology 134, 327–339.CrossRefGoogle ScholarPubMed
Ishida, N, Hobo, S, Takahashi, T, Nanbo, Y, Sato, F, Hasegawa, T, Mukoyama, H 1999. Chronological changes in superoxide-scavenging ability and lipid peroxide concentration of equine serum due to stress from exercise and transport. Equine Veterinary Journal Supplement 30, 430–433.CrossRefGoogle Scholar
Jarvis, AM, Cockram, MS 1994. Effects of handling and transport on bruising of sheep sent directly from farms to slaughter. Veterinary Record 135, 523–527.CrossRefGoogle ScholarPubMed
Jarvis, AM, Cockram, MS, McGilp, IM 1996. Bruising and biochemical measures of stress, dehydration and injury determined at slaughter in sheep transported from farms or markets. British Veterinary Journal 152, 719–722.CrossRefGoogle ScholarPubMed
Kaneko, JJ, Harvey, JW, Bruss, L 1997a. Appendixes. In Clinical biochemistry of domestic animals, 5th edition (ed. JJ Kaneko, JW Harvey and L Bruss), pp. 885–905. Academic Press, San Diego, USA.CrossRefGoogle Scholar
Kaneko, JJ 1997b. Serum proteins and dysproteinemias. In Clinical biochemistry of domestic animals, 5th edition (ed. JJ Kaneko, JW Harvey and L Bruss), pp. 117–155. Academic Press, San Diego, USA.CrossRefGoogle Scholar
Kirschvink, N, de Moffarts, B, Lekeux, P 2008. The oxidant/antioxidant equilibrium in horses. The Veterinary Journal 177, 178–191.CrossRefGoogle ScholarPubMed
Linares, MB, Bornez, R, Vergara, H 2008. Cortisol and catecholamine levels in lambs: effects of slaughter weight and type of stunning. Livestock Science 115, 53–61.CrossRefGoogle Scholar
McCord, JM, Fridovich, I 1969. The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen. Journal of Biological Chemistry 244, 6056–6063.CrossRefGoogle ScholarPubMed
McCorry, LK 2007. Physiology of the autonomic nervous system. American Journal of Pharmaceutical Education 71, 78.CrossRefGoogle ScholarPubMed
Micera, E, Albrizio, M, Surdo, NC, Moramarco, AM, Zarrilli, A 2010. Stress-related hormones in horses before and after stunning by captive bolt gun. Meat Science 84, 634–637.CrossRefGoogle ScholarPubMed
Miranda-de la Lama, GC, Rivero, L, Chacon, G, Garcia-Belenguer, S, Villarroel, M, Maria, GA 2010. Effect of the pre-slaughter logistic chain on some indicators of welfare in lambs. Livestock Science 128, 52–59.CrossRefGoogle Scholar
Mitchell, G, Hattingh, J, Ganhao, M 1988. Stress in cattle assessed after handling, after transport and after slaughter. Veterinary Record 123, 201–205.CrossRefGoogle ScholarPubMed
Moratinos, J, Reverte, M 1993. Effects of catecholamines on plasma potassium: the role of alpha- and beta-adrenoceptors. Fundamental & Clinical Pharmacology 7, 143–153.CrossRefGoogle ScholarPubMed
Muir, W 2004. Recognizing and treating pain in horses. In Equine internal medicine, 2nd edition (ed. SM Reed, WM Bayly and DC Sellon), pp. 1529–1541. Saunders, St Louis, MO, USA.CrossRefGoogle Scholar
Paglia, DE, Valentine, WN 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine 70, 158–169.Google ScholarPubMed
Shaw, FD, Tume, RK 1992. The assessment of pre-slaughter and slaughter treatments of livestock by measurements of plasma constituents – a review of recent work. Meat Science 32, 311–329.CrossRefGoogle ScholarPubMed
Stull, CJ 1999. Responses of horses to trailer design, duration, and floor area during commercial transportation to slaughter. Journal of Animal Science 77, 2925–2933.CrossRefGoogle ScholarPubMed
Stull, CL, Rodiek, AV 2000. Physiological responses of horses to 24 h of transportation using a commercial van during summer conditions. Journal of Animal Science 78, 1458–1466.CrossRefGoogle Scholar
Tadich, N, Gallo, C, Bustamante, H, Schwerter, M, van Schaik, G 2005. Effects of transport and lairage time on some blood constituents of Friesian-cross steers in Chile. Livestock Production Science 93, 223–233.CrossRefGoogle Scholar
Terlouw, EMC, Arnould, C, Auperin, B, Berri, C, Le Bihan-Duval, E, Deiss, V, Lefevre, F, Lensink, BJ, Mounier, L 2008. Pre-slaughter conditions, animal stress and welfare: current status and possible future research. Animal 2, 1501–1517.CrossRefGoogle ScholarPubMed
von Borell, EH 2001. The biology of stress and its application to livestock housing and transportation assessment. Journal of Animal Science 79 (E. suppl.), E260–E267.CrossRefGoogle Scholar
Werner, M, Gallo, C 2008. Effects of transport, lairage and stunning on the concentrations of some blood constituents in horses destined for slaughter. Livestock Science 115, 94–98.CrossRefGoogle Scholar
Wernicki, A, Urban-Chimel, R, Kankofer, M, Mikucki, P, Puchalski, A, Tokarzewski, S 2006. Evaluation of plasma cortisol and TBARS levels in calves after short-term transport. Revue de Medecine Veterinaire 157, 30–34.Google Scholar
Winther Christensen, J, Kelling, LJ, Lindestrøm Nielsen, B 2005. Responses of horses to novel visual, olfactory and auditory stimuli. Applied Animal Behaviour Science 93, 53–65.CrossRefGoogle Scholar
Young, JF, Rosenvold, K, Stagsted, J, Steffensen, CL, Nielsen, JH, Andersen, HJ 2003. Significance of preslaughter stress and different tissue PUFA levels on the oxidative status and stability of porcine muscle and meat. Journal of Agricultural and Food Chemistry 51, 6877–6881.CrossRefGoogle ScholarPubMed