Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-23T07:51:39.809Z Has data issue: false hasContentIssue false
  • English
  • Français

Selenium persistency and speciation in the tissues of lambs following the withdrawal of dietary high-dose selenium-enriched yeast

Published online by Cambridge University Press:  01 March 2008

D. T. Juniper ,
R. H. Phipps ,
E. Ramos-Morales  and
G. Bertin
D. T. Juniper*
Affiliation:
Animal Science Research Group, School of Agriculture, Policy and Development University of Reading, Earley Gate, Reading RG6 6AR, UK
R. H. Phipps
Affiliation:
Animal Science Research Group, School of Agriculture, Policy and Development University of Reading, Earley Gate, Reading RG6 6AR, UK
E. Ramos-Morales
Affiliation:
Animal Science Research Group, School of Agriculture, Policy and Development University of Reading, Earley Gate, Reading RG6 6AR, UK
G. Bertin
Affiliation:
ALLTECH France, EU Regulatory Affairs Department, 14 Place Marie-Jeanne Bassot, 92300 Levallois- Perret, France
*
E-mail: [email protected]
Get access

Abstract

The objective was to determine the concentration of total selenium (Se) and the proportion of total Se comprised as selenomethionine (SeMet) and selenocysteine (SeCys) in post mortem tissues of lambs in the 6 weeks period following the withdrawal of a diet containing high-dose selenised yeast (HSY), derived from a specific strain of Saccharomyces cerevisae CNCM (Collection Nationale de Culture de Micro-organism) I-3060. Thirty Texel × Suffolk lambs used in this study had previously received diets (91 days) containing either HSY (6.30 mg Se per kg dry matter (DM)) or an unsupplemented control (C; 0.13 mg Se per kg DM). Following the period of supplementation, all lambs were then offered a complete pelleted diet, without additional Se (0.15 mg Se per kg DM), for 42 days. At enrolment and 21 and 42 days later, five lambs from each treatment were blood sampled, euthanased and samples of heart, liver, kidney and skeletal muscle (longissimus dorsi and psoas major) tissue were retained. Total Se concentration in whole blood and tissues was significantly (P < 0.001) higher in HSY lambs at all time points that had previously received long-term exposure to high dietary concentrations of SY. The distribution of total Se and the proportions of total Se comprised as SeMet and SeCys differed between tissues, treatment and time points. Total Se was greatest in HSY liver and kidney (22.64 and 18.96 mg Se per kg DM, respectively) and SeCys comprised the greatest proportion of total Se. Conversely, cardiac and skeletal muscle (longissimus dorsi and psoas major) tissues had lower total Se concentration (10.80, 7.02 and 7.82 mg Se per kg DM, respectively) and SeMet was the predominant selenised amino acid. Rates of Se clearance in HSY liver (307 μg Se per day) and kidney (238 μg Se per day) were higher compared with HSY cardiac tissue (120 μg Se per day) and skeletal muscle (20 μg Se per day). In conclusion, differences in Se clearance rates were different between tissue types, reflecting the relative metabolic activity of each tissue, and appear to be dependent on the proportions of total Se comprised as either SeMet or SeCys.

Keywords

lambsseleniumselenocysteineselenomethioninetissues
Type
Full Paper
Information
animal , Volume 2 , Issue 3 , March 2008 , pp. 375 - 380
Copyright
Copyright © The Animal Consortium 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aspila, P 1988. Metabolism of selenite, selenomethionine and feed-incorporated selenium in lactating goats and dairy cows. Journal of Agricultural Science Finland 63, 1–74.Google Scholar
Commission Directive 2001/79/EC of 17 September 2001 amending Council Directive 87/153/EEC fixing guidelines for the assessment of additives in animal nutrition (Official Journal of the European Communities, L267–6.10.2001–1-26).Google Scholar
Cristaldi, LA, McDowell, LR, Buergelt, CD, Davis, PA, Wilkinson, NS, Martin, FG 2005. Tolerance of inorganic selenium in wether sheep. Small Ruminant Research 56, 205–213.CrossRefGoogle Scholar
Davis, PA, McDowell, LR, Wilkinson, NS, Buergelt, CD, Van Alstyne, R, Weldon, RN, Marshall, TT 2006. Tolerance of inorganic selenium by range-type ewes during gestation and lactation. Journal of Animal Science 84, 660–668.Google Scholar
Home Office 1986. Animal Scientific Procedures Act 1986. Her Majesty’s Stationary Office, London.Google Scholar
Juniper, DT, Phipps, RH, Jones, AK, Bertin, G 2006. Selenium supplementation of lactating dairy cows: Effect on selenium concentration in blood, milk, urine and feces. Journal of Dairy Science 89, 3544–3551.CrossRefGoogle ScholarPubMed
Korhola, M, Vainio, A, Edelmann, K 1986. Selenium yeast. Annals of Clinical Research 18, 65–68.Google ScholarPubMed
Lawler, TL, Taylor, JB, Finley, JW, Caton, JS 2004. Effect of supranutritional and organically bound selenium on performance carcass characteristics and selenium distribution in finishing beef steers. Journal of Animal Science 82, 1488–1493.Google Scholar
MacRae, JC, Armstrong, DG 1968. Enzyme method for determination of α-linked glucose polymers in biological materials. Journal of the Science and Food Agriculture 19, 578–581.Google Scholar
Ministry of Agriculture, Fisheries and Food 1982. The Feedingstuffs (sampling and analysis) Regulatory Instrument N. 1144. Pub. Her Majesty’s Stationery Office, London, UK.Google Scholar
Ministry of Agriculture, Fisheries and Food 1993. Prediction of energy values of compounds feedstuffs for farm animals bookler 1285. Pub. Her Majesty’s Stationery Office, London, UK.Google Scholar
Palacios, O, Encinar, JR, Bertin, G, Lobinski, P 2005. Analysis of the selenium species distribution in cow blood by size exclusion liquid chromatography – inductively coupled plasma collision cell mass spectrometry (SEC-ICPccMS). Analytical and Bioanalytical Chemistry 383, 516–522.CrossRefGoogle ScholarPubMed
Qin, S, Gao, J, Huang, K 2007. Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs. Biological Trace Element Research 116, 91–102.Google Scholar
Rayman, MP 2004. The use of high-selenium yeast to raise selenium status: how does it measure up? The British Journal of Nutrition 92, 557–573.CrossRefGoogle Scholar
Schubert, JR, Muth, OH, Oldfield, JE, Remmert, LF 1961. Experimental results with selenium in white muscle disease of lambs and calves. Federation Proceedings 20, 689–694.Google ScholarPubMed
Surai, PF 2006. Selenium absorption and metabolism. In Selenium nutrition and health, pp. 161–171.Nottingham University Press, Nottingham, UK.Google Scholar
Suzuki, KT, Ogra, Y 2002. Metabolic pathway for selenium in the body: speciation by HPLC-ICP MS with enriched Se. Food Additives and Contaminants 19, 974–983.Google Scholar
Taylor, JB 2005. Time-dependent influence of supranutritional organically bound selenium on selenium accumulation in growing whether lambs. Journal of Animal Science 83, 1186–1193.CrossRefGoogle Scholar
Weiss, WP 2003. Selenium nutrition of dairy cows: comparing responses to organic and inorganic selenium forms. In Proceeding 19th Alltech Annual Symposium. Nutritional Biotechnology in the Feed and Food Industries (ed. P Lyons and KA Jaques), pp. 333–343.Nottingham University Press, Nottingham, UK.Google Scholar