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Prion protein gene (PrP) polymorphisms in healthy sheep in Turkey

Published online by Cambridge University Press:  22 June 2011

Y. Oner ,
K. Yesilbag ,
E. Tuncel  and
C. Elmaci
Y. Oner*
Affiliation:
Department of Animal Science, Faculty of Agriculture, Uludag University, Bursa 16059, Turkey
K. Yesilbag
Affiliation:
Department of Microbiology, Faculty of Veterinary Medicine, Uludag University, Bursa 16059, Turkey
E. Tuncel
Affiliation:
Department of Animal Science, Faculty of Agriculture, Uludag University, Bursa 16059, Turkey
C. Elmaci
Affiliation:
Department of Animal Science, Faculty of Agriculture, Uludag University, Bursa 16059, Turkey
*
†E-mail: [email protected]
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Abstract

Scrapie, a transmissible spongiform encephalopathy (TSE) or prion disease, is a fatal, neurodegenerative disease in sheep and goats. This disease has been known in Europe for more than 250 years. Susceptibility to scrapie is associated with polymorphisms in the sheep prion protein gene (PrP) gene. In sheep, polymorphism in the PrP gene has been identified at a number of codons, and polymorphisms at codons 136, 154 and 171 have reported linkage with susceptibility to scrapie. Polymorphisms at the PrP locus were studied in 413 animals representing three native sheep breeds (Imroz, Chios and Kıvırcık) in Turkey. Genomic DNA was obtained from blood, and genotypes were screened using PCR and direct DNA sequencing. We report 17 genotypes derived from seven different alleles. The most frequent genotype in the Kıvırcık sheep is ARQ/ARQ, whereas the ARR/ARQ genotype is predominant in the Chios and Imroz breeds. In general, the ARQ haplotype was the predominant haplotype. ARQ haplotype was also predominant in the Kıvırcık and Chios sheep breeds, whereas the Imroz sheep predominantly had the ARR haplotype. The susceptibility-associated VRQ haplotype was found in 2.38%, 0.35% and 0.81% of the Imroz, Kıvırcık and Chios sheep, respectively. Moreover, seven additional polymorphisms have been detected at codons G127S, G127V, H143R, G145S, Y172D, N174Y and Q189L. Among these polymorphisms, the N174Y allele is a novel polymorphism, and the G145S allele is a novel allele for a known polymorphic locus.

Keywords

sheepscrapiePrPpolymorphismsusceptibility
Type
Full Paper
Information
animal , Volume 5 , Issue 11 , 26 September 2011 , pp. 1728 - 1733
Copyright
Copyright © The Animal Consortium 2011

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