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Post-hatching ontogeny of intestinal proton-coupled folate transporter and reduced folate carrier in broiler chickens

Published online by Cambridge University Press:  28 June 2013

M. Jing ,
G. B. Tactacan  and
J. D. House
M. Jing
Affiliation:
Department of Animal Science, University of Manitoba, Winnipeg, CanadaR3T 2N2 Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, CanadaR3T 2N2
G. B. Tactacan
Affiliation:
Department of Animal Science, University of Manitoba, Winnipeg, CanadaR3T 2N2
J. D. House*
Affiliation:
Department of Animal Science, University of Manitoba, Winnipeg, CanadaR3T 2N2 Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, CanadaR3T 2N2
*
†E-mail: [email protected]
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Abstract

Folate transporters, including the reduced folate carrier and the proton-coupled folate transporter, encoded by Slc19a1 and Slc46a1 genes respectively, play important roles in the transport of folate across biological membranes given the hydrophilic nature of folates. Although a number of studies have demonstrated that these two transporters are regulated ontogenetically in mammals, little data are available on their developmental patterns of expression in poultry. The objective of this study was to investigate the expression patterns of Slc19a1 and Slc46a1 in jejunal and cecal tissue of broiler chickens during post-hatching development. Post-hatch male chicks (Ross × Ross) had free access to water and a soybean/wheat-based diet. Jejunal, cecal and blood samples were collected on day-of-hatch but before feeding (D0), and on D2, D7, D14, D21 and D35 post-hatch (n = 8 at each time point), respectively. Plasma folate concentrations were low on the day of hatch and increased with maturation; by contrast, plasma homocysteine, a marker of folate status, was highest (P < 0.05) in the day-of-hatch birds and decreased thereafter. Increasing age reduced mRNA abundance of Slc19a1 (P < 0.05) in the jejunum and cecum. Abundance of Slc46a1 mRNA (P < 0.05) gradually decreased in the cecum with increasing age and that of Slc46a1 in the jejunum initially decreased and then increased to level similar to that of day-of-hatch. The study provides some initial data on ontogenetic regulation of Slc19a1 and Slc46a1 in the jejunum and cecum of the chicken and lays the ground work for future nutritional studies. Moreover, the expression of Slc19a1 and Slc46a1 transcripts in the cecum provides evidence of the potential for cecally derived folate to contribute to the folate status of the host.

Keywords

reduced folate carrierproton-coupled folate transportermRNA abundanceontogenybroiler
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 10 , October 2013 , pp. 1659 - 1664
Copyright
Copyright © The Animal Consortium 2013 

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References

Araki, A, Sako, Y 1987. Determination of free and total homocysteine in human plasma by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography 422, 43–52.Google Scholar
Ashokkumar, B, Mohammed, ZM, Vaziri, ND, Said, HM 2007. Effect of folate oversupplementation on folate uptake by human intestinal and renal epithelial cells. The American Journal of Clinical Nutrition 86, 159–166.Google Scholar
Asrar, FM, O'Connor, DL 2005. Bacterially synthesized folate and supplemental folic acid are absorbed across the large intestine of piglets. The Journal of Nutritional Biochemistry 16, 587–593.Google Scholar
Aviagen 2007. Ross 708 broiler: performance objectives, Section g (08–09), June 2007, Aviagen.Google Scholar
Balamurugan, K, Said, HM 2003. Ontogenic regulation of folate transport across rat jejunal brush-border membrane. American Journal of Physiology-Gastrointestinal and Liver Physiology 285, G1068–G1073.Google Scholar
Balamurugan, K, Ashokkumar, B, Moussaif, M, Sze, JY, Said, HM 2007. Cloning and functional characterization of a folate transporter from the nematode caenorhabditis elegans. American Journal of Physiology-Cell Physiology 293, C670–C681.Google Scholar
Canadian Council on Animal Care 1993. Guide to the care and use of experimental animals. Canadian Council on Animal Care, Ottawa, Ontario, Canada.Google Scholar
Chiao, JH, Roy, K, Tolner, B, Yang, CH, Sirotnak, FM 1997. RFC-1 gene expression regulates folate absorption in mouse small intestine. The Journal of Biological Chemistry 272, 11165–11170.CrossRefGoogle ScholarPubMed
Crittenden, RG, Martinez, NR, Playne, MJ 2003. Synthesis and utilisation of folate by yoghurt starter cultures and probiotic bacteria. International Journal of Food Microbiology 80, 217–222.Google Scholar
Dev, S, Ahmad Wani, N, Kaur, J 2011. Regulatory mechanisms of intestinal folate uptake in a rat model of folate oversupplementation. British Journal of Nutrition 105, 827–835.Google Scholar
Gilbert, ER, Li, H, Emmerson, DA, Webb, KE Jr, Wong, EA 2007. Developmental regulation of nutrient transporter and enzyme mRNA abundance in the small intestine of broilers. Poultry Science 86, 1739–1753.Google Scholar
Gilfix, BM, Blank, DW, Rosenblatt, DS 1997. Novel reductant for determination of total plasma homocysteine. Clinical Chemistry 43, 687–688.Google Scholar
House, JD, O'Connor, CP, Guenter, W 2003. Plasma homocysteine and glycine are sensitive indices of folate status in a rodent model of folate depletion and repletion. Journal of Agricultural and Food Chemistry 51, 4461–4467.Google Scholar
House, JD, Jacobs, RL, Stead, LM, Brosnan, ME, Brosnan, JT 1999. Regulation of homocysteine metabolism. Advances in Enzyme Regulation 39, 69–91.CrossRefGoogle ScholarPubMed
Inoue, K, Nakai, Y, Ueda, S, Kamigaso, S, Ohta, K, Hatakeyama, M, Hayashi, Y, Otagiri, M, Yuasa, H 2008. Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model. American Journal of Physiology-Gastrointestinal and Liver Physiology 294, G660–G668.Google Scholar
Jing, M, Tactacan, GB, Rodriguez-Lecompte, JC, Kroeker, A, House, JD 2009. Molecular cloning and tissue distribution of reduced folate carrier (RFC) and effect of dietary folate supplementation on the expression of RFC in laying hens. Poultry Science 88, 1939–1947.Google Scholar
Jing, M, Tactacan, GB, Rodriguez-Lecompte, JC, Kroeker, A, House, JD 2010. Proton-coupled folate transporter (PCFT): molecular cloning, tissue expression patterns and the effects of dietary folate supplementation on mRNA expression in laying hens. British Poultry Science 51, 635–638.Google Scholar
Kim, TH, Yang, J, Darling, PB, O'Connor, DL 2004. A large pool of available folate exists in the large intestine of human infants and piglets. The Journal of Nutrition 134, 1389–1394.Google Scholar
Li, H, Gilbert, ER, Zhang, Y, Crasta, O, Emmerson, D, Webb, KE Jr, Wong, EA 2008. Expression profiling of the solute carrier gene family in chicken intestine from the late embryonic to early post-hatch stages. Animal Genetics 39, 407–424.Google Scholar
Liu, M, Ge, Y, Cabelof, DC, Aboukameel, A, Heydari, AR, Mohammad, R, Matherly, LH 2005. Structure and regulation of the murine reduced folate carrier gene: identification of four noncoding exons and promoters and regulation by dietary folates. The Journal of Biological Chemistry 280, 5588–5597.Google Scholar
Lucock, M 2000. Folic acid: nutritional biochemistry, molecular biology, and role in disease processes. Molecular Genetics and Metabolism 71, 121–138.Google Scholar
Matherly, LH, Goldman, DI 2003. Membrane transport of folates. Vitamins and Hormones 66, 403–456.Google Scholar
Qiu, A, Jansen, M, Sakaris, A, Min, SH, Chattopadhyay, S, Tsai, E, Sandoval, C, Zhao, R, Akabas, MH, Goldman, ID 2006. Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption. Cell 127, 917–928.Google Scholar
Qiu, A, Min, SH, Jansen, M, Malhotra, U, Tsai, E, Cabelof, DC, Matherly, LH, Zhao, R, Akabas, MH, Goldman, ID 2007. Rodent intestinal folate transporters (SLC46A1): secondary structure, functional properties, and response to dietary folate restriction. American Journal of Physiology-Cell Physiology 293, C1669–C1678.Google Scholar
Said, HM 2004. Recent advances in carrier-mediated intestinal absorption of water-soluble vitamins. Annual Review of Physiology 66, 419–446.Google Scholar
Said, HM, Seetharam, B 2006. Intestinal absorption of water-soluble vitamins. In Physiology of the Gastrointestinal Tract, 4th edition (ed. LR Johnson), pp. 1791–1825. Elsevier, San Diego, CA.Google Scholar
Said, HM, Ghishan, FK, Murrell, JE 1985. Ontogenesis of intestinal transport of 5-methyltetrahydrofolate in the rat. American Journal of Physiology-Gastrointestinal and Liver Physiology 249, G567–G571.Google Scholar
Said, HM, Ghishan, FK, Redha, R 1987. Folate transport by human intestinal brush-border membrane vesicles. American Journal of Physiology-Gastrointestinal and Liver Physiology 252, G229–G236.Google Scholar
Said, HM, Chatterjee, N, Haq, RU, Subramanian, VS, Ortiz, A, Matherly, LH, Sirotnak, FM, Halsted, C, Rubin, SA 2000. Adaptive regulation of intestinal folate uptake: effect of dietary folate deficiency. American Journal of Physiology-Cell Physiology 279, C1889–C1895.Google Scholar
Shafizadeh, TB, Halsted, CH 2009. Postnatal ontogeny of intestinal GCP II and RFC in pig. American Journal of Physiology-Gastrointestinal and Liver Physiology 296, G476–G481.Google Scholar
Steel, RGD, Torrie, JH 1980. Principle and procedure of statistics. In A biochemical approach, 2nd edition. McGraw-Hill Books Company, New York, NY.Google Scholar
Subramanian, VS, Reidling, JC, Said, HM 2008. Differentiation-dependent regulation of the intestinal folate uptake process: studies with Caco-2 cells and native mouse intestine. American Journal of Physiology-Cell Physiology 295, C828–C835.Google Scholar
Tactacan, GB, Rodriguez-Lecompte, JC, Karmin, O, House, JD 2011. Functional characterization of folic acid transport in the intestine of the laying hen using the everted intestinal sac model. Poultry Science 90, 83–90.Google Scholar
van der Put, NM, van Straaten, HW, Trijbels, FJ, Blom, HJ 2001. Folate, homocysteine and neural tube defects: an overview. Experimental Biology and Medicine 226, 243–270.Google Scholar
Wang, Y, Rajgopal, A, Goldman, ID, Zhao, R 2005. Preservation of folate transport activity with a low-pH optimum in rat IEC-6 intestinal epithelial cell lines that lack reduced folate carrier function. American Journal of Physiology-Cell Physiology 288, C65–C71.Google Scholar