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Pig identification and meat traceability by multiallelic amplification fragments with multiple single nucleotide polymorphisms

Published online by Cambridge University Press:  22 December 2017

G. D. Xing ,
Y. N. Hu ,
Q. Ding ,
X. X. Wang ,
F. Xing ,
H. L. Wang ,
H. L. Huan  and
Y. X. Xu
G. D. Xing*
Affiliation:
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
Y. N. Hu
Affiliation:
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
Q. Ding
Affiliation:
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
X. X. Wang
Affiliation:
Model Animal Research Center, Nanjing University, Nanjing, Jiangsu 210061, China
F. Xing
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China
H. L. Wang
Affiliation:
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
H. L. Huan
Affiliation:
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
Y. X. Xu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
*
†E-mail: [email protected]
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Abstract

Compared with conventional identification methods, DNA-based genetic approaches such as single nucleotide polymorphisms (SNPs) and satellites are much more reliable for pig identification and meat traceability. In this study, multiallelic amplification fragments with multiple SNPs, incorporating the advantages of both SNPs and microsatellites, were explored for the first time for pig identification and meat traceability. Primer pairs for multiallelic fragments and their optimal SNPs were successfully selected and used for identification of individuals from Suzhong and Duroc populations. Meanwhile, the combined panel of the above mentioned primer pairs together with their optimal SNPs for Suzhong and/or Duroc pigs were validated for identification of the hybrids (Suzhong×Duroc). Therefore, we have successfully selected multiallelic amplification fragments with multiple SNPs to identify pigs and their meat samples from Suzhong, Duroc or their hybrids. Our study demonstrates that our method is more powerful for pig identification or meat traceability than SNPs or microsatellites.

Keywords

pig identificationmeat traceabilityamplification fragmentsingle nucleotide polymorphismmicrosatellite
Type
Research Article
Information
animal , Volume 12 , Issue 9 , September 2018 , pp. 1785 - 1791
Copyright
© The Animal Consortium 2017 

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Footnotes

a

Present address: Institute of Zoology, Chinese Academy of Science, Beijing, 100101, China.

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