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Peripheral cytokine expression in Standardbred mares at different adiposity during the periparturient period

Published online by Cambridge University Press:  22 June 2011

R. Saleri ,
A. Sabbioni ,
I. Vecchi ,
I. Davighi ,
F. Vaccari Simonini  and
P. Superchi
R. Saleri*
Affiliation:
Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy
A. Sabbioni
Affiliation:
Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy
I. Vecchi
Affiliation:
Dipartimento di Salute Animale, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy
I. Davighi
Affiliation:
Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy
F. Vaccari Simonini
Affiliation:
Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy
P. Superchi
Affiliation:
Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy
*
†E-mail: [email protected]
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Abstract

The aim of this study was to evaluate whether the peripheral cytokine expression could be affected by differences in fat thickness in healthy mares during the periparturient period (last weeks of pregnancy and first weeks of lactation). At the beginning of the 11th month of gestation, 18 mares with normal body condition score (BCS; 5) were divided into two groups with low (L; ⩽12 mm; n = 12) and high (H; >12 mm; n = 6) fat thickness. Blood samples were collected every 10 days from the 11th month of gestation to 20 days post partum. Peripheral blood mononuclear cells were separated and tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1α (IL-1α), -1β (ILβ), -2 (IL-2) and -6 (IL-6) gene expressions were performed by using reverse transcriptase PCR. TNF-α and IFN-γ expressions were affected by fatness group, as the sampling period influenced the expression of all cytokines with the exception of IFN-γ. The interaction between fatness group and sampling period tended to be significant (P < 0.10) only for IL-1β, TNF-α and IL-2. In H group, compared with L group, IL-1β and IL-2 significantly (P < 0.05) increased at parturition. TNF-α peripheral expression increased from parturition to reach the peak at +10 days (P < 0.05) only in H group. In conclusion, in normal BCS periparturient mares, small body fat thickness variations influence the peripheral cytokine expression, showing a possible role for cytokines in parturition and lactation, events strongly linked to adipose tissue availability.

Keywords

mareperiparturient periodcytokine expressionadiposity
Type
Full Paper
Information
animal , Volume 5 , Issue 12 , 10 November 2011 , pp. 1938 - 1943
Copyright
Copyright © The Animal Consortium 2011

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References

Adams, AA, Katepalli, MP, Kohler, K, Reedy, SE, Stilz, JP, Vick, MM, Fitzgerald, BP, Lawrence, LM, Horohov, DW 2009. Effect of body condition, body weight and adiposity on inflammatory cytokine responses in old horses. Veterinary Immunology and Immunopathology 127, 286–294.CrossRefGoogle ScholarPubMed
Alexaki, VI, Notas, G, Pelekanou, V, Kampa, M, Valkanou, M, Theodoropoulos, P, Stathopoulos, EN, Tsapis, A, Castanas, E 2009. Adipocytes as immune cells: differential expression of TWEAK, BAFF, and APRIL and their receptors (Fn14, BAFF-R, TACI, and BCMA) at different stages of normal and pathological adipose tissue development. Journal of Immunology 183, 5948–5956.CrossRefGoogle ScholarPubMed
Berkenbosch, F, van Oers, J, del Rey, A, Tilders, F, Besedovsky, H 1987. Corticotropin-releasing factor-producing neurons in the rat activated by interleukin-1. Science 238, 524–526.CrossRefGoogle ScholarPubMed
Besedovsky, HO, del Rey, A 2000. The cytokine-HPA axis feed-back circuit. Zeitschrift für Rheumatologie 59, 26–30.CrossRefGoogle ScholarPubMed
Blue, MG, Brady, AA, Davidson, JN, Kenney, RM 1982. Studies on the composition and antibacterial activity of uterine fluid from mares. Journal of Reproduction and Fertility 32, 143–149.Google ScholarPubMed
Borghetti, P, Saleri, R, Mocchegiani, E, Corradi, A, Martelli, P 2009. Infection, immunity and the neuroendocrine response. Veterinary Immunology and Immunopathology 130, 141–162.CrossRefGoogle ScholarPubMed
Bowen, JM, Chamley, L, Keelan, JA, Mitchell, MD 2002. Cytokines of the placenta and extra-placental membranes: roles and regulation during human pregnancy and parturition. Placenta 23, 257–273.CrossRefGoogle ScholarPubMed
Challis, JR, Lockwood, CJ, Myatt, L, Norman, JE, Strauss, JF, Petraglia, F 2009. Inflammation and pregnancy. Reproduction Science 16, 206–215.CrossRefGoogle ScholarPubMed
Coppack, SW 2001. Pro-inflammatory cytokines and adipose tissue. In Proceedings Nutrition Society 60 (ed. KR Westerterp), pp. 349–356. Maastricht University, The Netherlands.Google Scholar
Coulam, CH, Edwin, SS, LaMarche, S, Mitchell, MD 1993. Actions of interleukin-2 on chorio-decidual prostaglandin biosynthesis. Prostaglandins 46, 145–156.CrossRefGoogle ScholarPubMed
Desjardins, M 2009. The good fat: a link between lipid bodies and antigen cross-presentation. Immunity 31, 176–178.CrossRefGoogle ScholarPubMed
Frasier-Scott, K, Hatzakis, H, Seong, D, Jones, CM, Wu, KK 1988. Influence of natural and recombinant interleukin 2 on endothelial cell arachidonate metabolism. Induction of de novo synthesis of prostaglandin H synthase. The Journal of Clinical Investigation 82, 1877–1883.CrossRefGoogle ScholarPubMed
Giguère, S, Prescott, JF 1999. Quantitation of equine cytokine mRNA expression by reverse transcription-competitive polymerase chain reaction. Veterinary Immunology and Immunopathology 67, 1–15.CrossRefGoogle ScholarPubMed
Haas, SD, Bristol, F, Card, CE 1996. Risk factors associated with the incidence of foal mortality in an extensively managed mare herd. Canadian Veterinary Journal 37, 91–95.Google Scholar
Harvey, NL 2008. The link between lymphatic function and adipose biology. Annals of the New York Academy of Sciences 1131, 82–88.CrossRefGoogle ScholarPubMed
Hauner, H, Petruschke, T, Russ, M, Eckel, J 1995. Effects of tumour necrosis factor alpha (TNF) on glucose transport and lipid metabolism of newly-differentiated human fat cells in cell culture. Diabetologia 38, 764–771.CrossRefGoogle ScholarPubMed
Henneke, DR, Potter, GD, Kreider, JL, Yeates, BF 1983. Relationship between condition score, physical measurements and body fat percentage in mares. Equine Veterinary Journal 15, 371–372.CrossRefGoogle ScholarPubMed
Kajantie, E, Dunkel, L, Turpeinen, U, Stenman, UH, Wood, PJ, Nuutila, M, Andersson, S 2003. Placental 11ß-hydroxysteroid dehydrogenase-2 and fetal cortisol/cortisone shuttle in small preterm infants. The Journal of Clinical Endocrinology and Metabolism 88, 493–500.CrossRefGoogle Scholar
Keelan, JA, Blumenstein, M, Helliwell, RJ, Sato, TA, Marvin, KW, Mitchell, MD 2003. Cytokines, prostaglandins and parturition – a review. Placenta 24 (suppl. 1), S33–S46.CrossRefGoogle ScholarPubMed
Morell, V 1995. Attacking the causes of “silent” infertility. Science 269, 775–777.CrossRefGoogle ScholarPubMed
Nishimura, S, Manabe, I, Nagai, R 2009. Adipose tissue inflammation in obesity and metabolic syndrome. Discovery Medicine 8, 55–60.Google ScholarPubMed
Ogawa, H, Nielsen, S, Kawakami, M 1989. Cachetin/tumor necrosis factor and interleukin-1 show different modes of combined effect on lipoprotein lipase and intracellular lipolysis. Biochimica et Biophysica Acta 1003, 131–135.CrossRefGoogle Scholar
Olsen, NJ, Kovacs, WJ 1996. Gonadal steroids and immunity. Endocrine Reviews 17, 369–384.Google ScholarPubMed
Ousey, JC 2004. Peripartal endocrinology in the mare and foetus. Reproduction in Domestic Animals 39, 222–231.CrossRefGoogle ScholarPubMed
Ousey, JC 2006. Hormone profiles and treatments in the late pregnant mare. Veterinary Clinics of North America: Equine Practice 22, 727–747.Google ScholarPubMed
Saeed, SA, Qureshi, Z, Saleem, A, Quadri, J, Sheikh, AF, Waqar, MA 2008. Prostaglandins and cytokines in pregnancy and labor. Journal of the Chemical Society of Pakistan 30, 963–971.Google Scholar
Sapolsky, R, Rivier, C, Yamamoto, G, Plotsky, P, Vale, W 1987. Interleukin-1 stimulates the secretion of hypothalamic corticotropin-releasing factor. Science 238, 522–524.CrossRefGoogle ScholarPubMed
SAS 2003. User's guide: statistics. Version 9.1.3. SAS Institute Inc., Cary, NC, USA.Google Scholar
Stephens, JM, Pekala, PH 1991. Transcriptional regression of the GLUT4 and C/EBP genes in 3T3-L1 adipocytes by tumor necrosis factor-alpha. The Journal of Biological Chemistry 266, 21839–21845.CrossRefGoogle Scholar
Sumida, M, Shiosaka, T, Nagar, A, Isshikimasuda, M, Okuda, H, Hamada, M 1997. Suppressive effect of tumor necrosis factor on adipogenic cell differentiation and on gene expression of hormone-sensitive lipase. Journal of Clinical Biochemistry and Nutrition 22, 1–11.CrossRefGoogle Scholar
Tilg, H, Moschen, AR 2006. Adipocytokines: mediators linking adipose tissue, inflammation and immunity. Nature Reviews Immunology 6, 772–783.CrossRefGoogle ScholarPubMed
Trayhurn, P, Wood, IS 2004. Adipokines: inflammation and the pleiotropic role of white adipose tissue. British Journal of Nutrition 92, 347–355.CrossRefGoogle ScholarPubMed
Vick, MM, Adams, AA, Murphy, BA, Sessions, DR, Horohov, DW, Cook, RF, Shelton, BJ, Fitzgerald, BP 2007. Relationships among inflammatory cytokines, obesity, and insulin sensitivity in the horse. Journal of Animal Science 85, 1144–1155.CrossRefGoogle ScholarPubMed
Weisberg, SP, McCann, D, Desai, M, Rosenbaum, M, Leibel, RL, JrFerrante, AW 2003. Obesity is associated with macrophage accumulation in adipose tissue. The Journal of Clinical Investigation 112, 1796–1808.CrossRefGoogle ScholarPubMed
Zarković, M, Ignjatović, S, Dajak, M, Cirić, J, Beleslin, B, Savić, S, Stojković, M, Bulat, P, Trbojević, B 2008. Cortisol response to ACTH stimulation correlates with blood interleukin 6 concentration in healthy humans. European Journal of Endocrinology 159, 649–652.CrossRefGoogle ScholarPubMed