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Oral and intranasal administration of somatostatin DNA vaccine mediated by attenuated SalmonellaEnterica Serovar Typhimurium to promote growth of piglets

Published online by Cambridge University Press:  22 February 2011

X. B. Liu ,
A. X. Liang ,
X. G. Feng ,
A. Z. Guo ,
C. Y. Ke ,
S. J. Zhang  and
L. G. Yang
X. B. Liu
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
A. X. Liang
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
X. G. Feng
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
A. Z. Guo
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
C. Y. Ke
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
S. J. Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
L. G. Yang*
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
*
†E-mail: [email protected]
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Abstract

An attenuated strain of Salmonella typhimurium has been used as a carrier for oral and intranasal genetic immunization. Here, we evaluate the efficacy of a vaccine strain of S. typhimurium. CSO22 (pGM-CSF/SS, plasmid granulocyte-macrophage colony-stimulating factor/somatostatin) expressing two copies of SS genes. A total of 115 piglets, aged 2 months old, were either orally or intranasally immunized against the vaccine strain CSO22 (pGM-CSF/SS) with three dosages (5 × 1010 colony forming units (CFU), 5 × 109 CFU and 5 × 108 CFU). For oral immunization, the specific anti-SS antibodies were detected in the immunized piglets. The levels of SS antibodies in the high-dose immunized group (5 × 1010 CFU) were significantly higher than that in the phosphate buffered saline immunized group (P < 0.01) and 40% of animals were positive in SS antibodies in the high-dose immunized group. Moreover, the weight gain of the high-dose group was increased by 20.86%, 10.26% and 15.30% during 4, 8 and 12 weeks, respectively, after immunization in comparison to the control. For intranasal immunization, the growth of the low-dose group was increased by 10.23% in the whole test period (12 weeks). In conclusion, our results suggest that the recombinant strain could elicit anti-SS antibodies and improve the growth performance of immunized piglets, and that the oral immunization program is better than the intranasal program.

Keywords

salmonellasomatostatinDNA vaccinepiglets
Type
Full Paper
Information
animal , Volume 5 , Issue 8 , August 2011 , pp. 1231 - 1236
Copyright
Copyright © The Animal Consortium 2011

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Footnotes

a

The authors contributed equally to this paper.

References

Buonomo, FC, Sabacky, MJ, Della-Fera, MA, Baile, CA 1987. Effects of somatostatin immunoneutralization on growth and endocrine parameters in chickens. Domestic Animal Endocrinology 4, 191–200.CrossRefGoogle ScholarPubMed
Dawson, JM, Soar, JB, Buttery, PJ, Craigon, J, Gill, M, Beever, DE 1997. The effect of immunization against somatostatin and β-agonist administration alone and in combination on growth and carcass composition in young steers. Animal Science 64, 37–51.CrossRefGoogle Scholar
Donnelly, JJ, Wahren, B, Liu, MA 2005. DNA vaccines: progress and challenges. Journal of Immunology 175, 633–639.CrossRefGoogle ScholarPubMed
Elsaesser, F, Drath, S 1995. The potential of immuno-neutralization of somatostatin for improving pig performance. Livestock Production Science 42, 255–263.CrossRefGoogle Scholar
Fló, J, Tisminetzky, S, Baralle, F 2001. Oral transgene vaccination mediated by attenuated Salmonellae is an effective method to prevent Herpes simplex virus-2 induced disease in mice. Vaccine 19, 1772–1782.CrossRefGoogle ScholarPubMed
Hameleers, DM, van der Ven, I, Biewenga, J, Sminia, T 1991. Mucosal and systemic antibody formation in the rat after intranasal administration of three different antigens. Immunology and Cell Biology 69, 119–125.CrossRefGoogle ScholarPubMed
Han, L, Liang, AX, Zhang, J, Fang, M, Hua, GH, Sang, L, Geng, LY, Wang, HR, Yang, LG 2008. Evaluation of the VP22 gene adjuvant for enhancement of DNA vaccine against somatostatin in mice. Animal 2, 1569–1574.CrossRefGoogle ScholarPubMed
Leitner, WW, Ying, H, Restifo, NP 1999. DNA and RNA-based vaccines: principles, progress and prospects. Vaccine 18, 765–777.CrossRefGoogle ScholarPubMed
Li, GX, Qiu, HJ, Han, CG, Han, LX, Zhou, YJ, Tong, GZ 2006. Vaccination of plasmid DNA encoding somatostatin gene fused with GP5 gene of porcine reproductive and respiratory syndrome virus induces anti-GP5 antibodies and promotes growth performance in immunized pigs. Agricultural Sciences in China 5, 234–240.CrossRefGoogle Scholar
Liang, AX, Yao, YF, Jin, DE, Wu, R, Han, L, Yang, LG 2006. The immune response of mice against pGM-CSF/SS somatostatin DNA vaccine. Journal of Huazhong Agricultural University 25, 540–543.Google Scholar
Liang, AX, Cao, SX, Han, L, Yao, YF, Moaeen-Ud-Din, M, Yang, LG 2008. Construction and evaluation of the eukaryotic expression plasmid encoding two copies of somatostatin genes fused with hepatitis B surface antigen gene S. Vaccine 26, 2935–2941.CrossRefGoogle ScholarPubMed
Redman, TK, Harmon, CC, Michalek, SM 1996. Oral immunization with recombinant Salmonella typhimurium expressing surface protein antigen A (SpaA) of Streptococcus sobrinus: effects of the Salmonella virulence plasmid on the induction of protective and sustained humoral responses in rats. Vaccine 14, 868–878.CrossRefGoogle ScholarPubMed
Roberts, JA, Sockett, PN 1994. The socioeconomic impact of human Salmonella enteritidis infection. International Journal of Food Microbiology 21, 117–129.CrossRefGoogle ScholarPubMed
Schoen, C, Stritzker, J, Goebel, W, Pilgrim, S 2004. Bacteria as DNA vaccine carriers for genetic immunization. International Journal of Medical Microbiology 294, 319–335.CrossRefGoogle ScholarPubMed
Shu, DQ, Mao, DG, Wu, ZM, Cheng, B, Yang, LG 2006. Lymphocyte proliferation and GH, IGF-I secretion of mice immunized with fusion-expression plasmid of GM-CSF and somatostatin delivered by attenuated Salmonella. Acta Veterinaria et Zootechnica Sinica 37, 814–818.Google Scholar
Shu, DQ, Mao, DG, Wu, ZM, Chen, RD, Yang, LG 2008. Construction and immunization of Fusion-expression Plasmid Harboring Porcine GM-CSF and somatostatin. Journal of Agricultural Biotechnology 16, 41–46.Google Scholar
Shulkes, A, Moore, C, Kolivas, S, Whitley, J 1999. Active immunoneutralization of somatostatin in the sheep: effects on gastrointestinal somatostatin expression, storage and secretion. Regulatory Peptides 82, 59–64.CrossRefGoogle ScholarPubMed
Sirard, JC, Niedergang, F, Kraehenbuhl, JP 1999. Live attenuated salmonella: a paradigm of mucosal vaccines. Immunological Reviews 171, 5–26.CrossRefGoogle ScholarPubMed
Smith, BP, Dilling, GW, Da Roden, L, Stocker, BA 1993. Vaccination of calves with orally administered aromatic-dependent Salmonella dublin. American Journal of Veterinary Research 54, 1249–1255.CrossRefGoogle ScholarPubMed
Spencer, GSG 1986. Immuno-neutralization of somatostatin (SS) and its effect on animal production. Domestic Animal Endocrinology 3, 55–68.CrossRefGoogle Scholar
Spreng, S, Dietrich, G, Weidinger, G 2006. Rational design of Salmonella-based vaccination strategies. Methods 38, 133–143.CrossRefGoogle ScholarPubMed
Weiss, S 2003. Transfer of eukaryotic expression plasmids to mammalian hosts by attenuated salmonella spp. International Journal of Medical Microbiology 293, 95–106.CrossRefGoogle ScholarPubMed
Yang, LG, Hu, SC, Wei, PH, Guo, AZ 1998. Enzyme immunoassay technology. Nanjing University Press, Nanjing, China.Google Scholar
Yun, CH, Estrada, A, Gajadhar, AA, Redmond, MJ, Laarveld, B 1996. Passive immunization against somatostatin increases resistance to Eimeria vermiformis infection in susceptible mice. Comparative Immunology Microbiology and Infectious Diseases 19, 39–46.CrossRefGoogle ScholarPubMed