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Modifications of the G6G timed-AI protocol improved pregnancy per AI and reduced pregnancy loss in lactating dairy cows

Published online by Cambridge University Press:  15 March 2017

F. Heidari ,
E. Dirandeh ,
Z. Ansari Pirsaraei  and
M. G. Colazo
F. Heidari
Affiliation:
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, PO Box 578, Sari, Mazandaran, Iran
E. Dirandeh*
Affiliation:
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, PO Box 578, Sari, Mazandaran, Iran
Z. Ansari Pirsaraei
Affiliation:
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, PO Box 578, Sari, Mazandaran, Iran
M. G. Colazo
Affiliation:
Livestock Research Section, Alberta Agriculture and Forestry, Edmonton, AB, Canada, T6H 5T6
*
†E-mail: [email protected]
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Abstract

In dairy cows, subjected to a G6G protocol, objectives were to determine effects of (1) extending the interval from prostaglandin F2α (PGF2α) to gonadotropin-releasing hormone (GnRH) during presynchronization; and (2) adding a second PGF2α treatment before artificial insemination (AI), on ovarian response, plasma progesterone (P4) concentrations and pregnancy per AI (P/AI). In a 2×2 factorial design, lactating cows were randomly assigned to one of four timed AI (TAI) protocols: (1) G6G (n=149), one injection of PGF2α, GnRH 2 days later and a 7-day Ovsynch (GnRH, 7 days, PGF2α, 56 h, GnRH, 16 h, TAI) was initiated 6 days later; (2) G6GP (n=144), an additional PGF2α treatment (24 h after the first) during Ovsynch of the G6G protocol; (3) MG6G, one injection of PGF2α, GnRH 4 days later before initiation of the G6G protocol; and (4) MG6GP, an additional PGF2α treatment (24 h after the first) during Ovsynch of the MG6G protocol. Blood samples were collected (subset of 200 cows) at first GnRH and PGF2α of the Ovsynch, and at TAI to measure P4. Ultrasound examinations were performed in a subset of 406 cows to evaluate ovarian response at various times of Ovsynch, and in all cattle to determine pregnancy status at 32 and 60 days after TAI. Extending the interval by 2 days between PGF2α and GnRH during presynchronization increased (P<0.01) ovulatory response to first GnRH of Ovsynch, circulating P4 during Ovsynch, and P/AI at 32 and 60 days after TAI. Adding a second PGF2α treatment before AI increased the proportion of cows with luteal regression (P=0.04), improved P/AI at 60 days after TAI (P=0.05), and reduced pregnancy loss between 30 and 60 days after TAI (P=0.04). In summary, extending the interval from PGF2α to GnRH during presynchronization increased response to first GnRH of Ovsynch and P4 concentrations during Ovsynch, whereas adding a second PGF2α treatment before AI enhanced luteal regression. Both modifications of the G6G protocol improved fertility in lactating dairy cows.

Keywords

timed AIpregnancypregnancy lossPGF2αdairy cow
Type
Research Article
Information
animal , Volume 11 , Issue 11 , November 2017 , pp. 2002 - 2009
Copyright
© The Animal Consortium and Her Majesty the Queen in Right of Canada, represented by the Minister of Agriculture and Agri-Food Canada and the Minister of Health Canada 2017 

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References

Behrouzi, A, López-Helguera, I, Mapletoft, RJ, Ambrose, DJ and Colazo, MG 2016. Plasma progesterone concentration at TAI in dairy cows: what is low enough? Proceedings of the 18th International Congress on Animal Reproduction, 26–30 June 2016, Tours, France.Google Scholar
Bello, NM, Steibel, JP and Pursley, JR 2006. Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of Ovsynch in lactating dairy cows. Journal of Dairy Science 89, 3413–3424.Google Scholar
Bridges, PJ and Fortune, JE 2003. Characteristics of developing prolonged dominant follicles in cattle. Domestic Animal Endocrinology 25, 199–214.Google Scholar
Brusveen, DJ, Cunha, AP, Silva, CD, Cunha, PM, Sterry, RA, Silva, EP, Guenther, JN and Wiltbank, MC 2008. Altering the time of the second gonadotropin-releasing hormone injection and artificial insemination (AI) during Ovsynch affects pregnancies per AI in lactating dairy cows. Journal of Dairy Science 91, 1044–1052.CrossRefGoogle ScholarPubMed
Brusveen, DJ, Souza, AH and Wiltbank, MC 2009. Effects of additional prostaglandin F2α and estradiol-17 β during Ovsynch in lactating dairy cows. Journal of Dairy Science 92, 1412–1422.Google Scholar
Carvalho, PD, Fuenzalida, MJ, Ricci, A, Souza, AH, Barletta, RV, Wiltbank, MC and Fricke, PM 2015. Modifications to Ovsynch improve fertility during resynchronization: evaluation of presynchronization with gonadotropin-releasing hormone 6 d before initiation of Ovsynch and addition of a second prostaglandin F2α treatment. Journal of Dairy Science 98, 8741–8752.Google Scholar
Chebel, RC, Santos, JE, Cerri, RL, Rutigliano, HM and Bruno, RG 2006. Reproduction in dairy cows following progesterone insert presynchronization and resynchronization protocols. Journal of Dairy Science 89, 4205–4219.Google Scholar
Colazo, MG, Behrouzi, A, Ambrose, DJ and Mapletoft, RJ 2015. Diameter of the ovulatory follicle at timed artificial insemination as a predictor of pregnancy status in lactating dairy cows subjected to GnRH-based protocols. Theriogenology 84, 377–383.Google Scholar
Colazo, MG, Dourey, A, Rajamahendran, R and Ambrose, DJ 2013a. Progesterone supplementation before timed AI increased ovulation synchrony and pregnancy per AI, and supplementation after timed AI reduced pregnancy losses in lactating dairy cows. Theriogenology 79, 833–841.Google Scholar
Colazo, MG and Mapletoft, RJ 2014. A review of current timed-AI (TAI) programs for beef and dairy cattle. Canadian Veterinary Journal 55, 772–780.Google Scholar
Colazo, MG, Ponce-Barajas, P and Ambrose, DJ 2013b. Pregnancy per AI in lactating dairy cows subjected to two different intervals from presynchronization to initiation of Ovsynch protocol. Journal of Dairy Science 96, 7640–7648.Google Scholar
Dirandeh, E 2014. Starting Ovsynch protocol on day 6 of first postpartum estrous cycle increased fertility in dairy cows by affecting ovarian response during heat stress. Animal Reproduction Science 149, 135–140.CrossRefGoogle ScholarPubMed
Dirandeh, E, Kohram, H and Zare Shahneh, A 2009. GnRH injection before artificial insemination (AI) alters follicle dynamics in Iranian Holstein cows. African Journal of Biotechnology 8, 3672–3676.Google Scholar
Dirandeh, E, Rezaei Roodbari, A, Gholizadeh, M, Deldar, H, Masoumi, R, Kazemifard, M and Colazo, MG 2015a. Administration of prostaglandin F2α 14 d before initiating a G6G or a G7G timed-AI protocol increased circulating progesterone prior to AI and reduced pregnancy loss in multiparous Holstein cows. Journal of Dairy Science 98, 5414–5421.Google Scholar
Dirandeh, E, Rezaei Roodbari, A and Colazo, MG 2015b. Double-Ovsynch, compared with presynch with or without GnRH, improves fertility in heat-stressed lactating dairy cows. Theriogenology 83, 438–443.Google Scholar
Ghanem, ME, Nakao, T, Nakatani, K, Akita, M and Suzuki, T 2006. Milk progesterone profile at and after artificial insemination in repeat-breeding cows: effects on conception rate and embryonic death. Reproduction in Domestic Animal 41, 180–183.Google Scholar
Ginther, OJ, Wiltbank, MC, Fricke, PM, Gibbons, JR and Kot, K 1996. Selection of the dominant follicle in cattle. Biology of Reproduction 55, 1187–1194.Google Scholar
Graham, JD and Clarke, CL 1997. Physiological action of progesterone in target tissues. Endocrinology Review 18, 502–519.Google Scholar
Gümen, A, Souza, AH, Cunha, AP, Silva, EBPC, Guenther, JN and Wiltbank, MC 2005. Effect of GnRH between presynch injections and estradiol-17β during the Ovsynch protocol on conception rates in lactating dairy cows. Journal of Animal Science 83, 171.Google Scholar
Howard, HJ and Britt, JH 1990. Prostaglandin F-2α causes regression of an hCG-induced corpus luteum before day 5 of its lifespan in cattle. Journal of Reproduction and Fertility 90, 245–253.CrossRefGoogle Scholar
Kastelic, JP and Ginther, OJ 1991. Factors affecting the origin of the ovulatory follicle in heifers with induced luteolysis. Animal Reproduction Science 26, 13–24.Google Scholar
Kinder, JE, Kojima, FN, Bergfeld, EGM, Wehrman, ME and Fike, KE 1996. Progestin and estrogen regulation of pulsatile LH release and development of persistent ovarian follicles in cattle. Journal of Animal Science 74, 1424–1440.CrossRefGoogle ScholarPubMed
Martinez, MF, Adams, GP, Bergfelt, DR, Kastelic, JP and Mapletoft, RJ 1999. Effect of LH or GnRH on the dominant follicle of the first follicular wave in beef heifers. Animal Reproduction Science 57, 23–33.Google Scholar
Martins, JPN, Policelli, RK, Neuder, LM, Raphael, W and Pursley, JR 2011. Effects of cloprostenol sodium at final prostaglandin F2a of Ovsynch on complete luteolysis and pregnancy per artificial insemination in lactating dairy cows. Journal of Dairy Science 94, 2815–2824.Google Scholar
Momont, HW and Seguin, BE 1982. Temporal factors affecting the response to prostaglandin F2a products in dairy cattle. Proceedings of the Annual Conference of the Society for Theriogenology 1, 166–167.Google Scholar
Moreira, F, de la Sota, RL, Diaz, T and Thatcher, WW 2000. Effect of day of the estrous cycle at the initiation of a timed artificial insemination protocol on reproductive responses in dairy heifers. Journal of Animal Science 78, 1568–1576.Google Scholar
Moreira, F, Orlandi, C, Risco, CA, Mattos, R, Lopes, F and Thatcher, WW 2001. Effects of presynchronization and bovine somatotropin on pregnancy rates to a timed artificial insemination protocol in lactating dairy cows. Journal of Dairy Science 84, 1646–1659.CrossRefGoogle ScholarPubMed
Motavalli, T, Dirandeh, E, Deldar, H and Colazo, MG 2017. Evaluation of shortened timed-AI protocols for resynchronization of ovulation in multiparous Holstein dairycows. Theriogenology (in press).Google Scholar
National Research Council (NRC) 2001. Nutrient requirements of dairy cattle, 7th edition. National Academy of Science, Washington, DC, USA.Google Scholar
Pursley, JR, Mee, MO and Wiltbank, MC 1995. Synchronization of ovulation in dairy cows using PGF2alpha and GnRH. Theriogenology 44, 915–923.CrossRefGoogle ScholarPubMed
Revah, I and Butler, WR 1996. Prolonged dominance of follicles and reduced viability of bovine oocytes. Journal of Reproduction and Fertility 106, 39–47.Google Scholar
Sartori, R, Fricke, PM, Ferreira, JCP, Ginther, OJ and Wiltbank, MC 2001. Follicular deviation and acquisition of ovulatory capacity in bovine follicles. Biology of Reproduction 65, 1403–1409.Google Scholar
Savio, JD, Thatcher, WW, Morris, GR, Entwistle, K, Drost, M and Mattiacci, MR 1993. Effects of induction of low plasma progesterone concentrations with a progesterone-releasing intravaginal device on follicular turnover and fertility in cattle. Journal of Reproduction and Fertility 98, 77–84.CrossRefGoogle ScholarPubMed
Souza, AH, Ayres, H, Ferreira, RM and Wiltbank, MC 2008. A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenology 70, 208–215.Google Scholar
Souza, AH, Gumen, A, Silva, EPB, Cunha, AP, Guenther, JN, Peto, CM, Caraviello, DZ and Wiltbank, MC 2007. Supplementation with estradiol-17beta before the last gonadotropin-releasing hormone injection of the ovsynch protocol in lactating dairy cows. Journal of Dairy Science 90, 4623–4634.Google Scholar
Wade, DE and Lewis, GS 1996. Exogenous prostaglandin F2α stimulates utero-ovarian release of prostaglandin F2α in sheep: a possible component of the luteolytic mechanism of action of exogenous prostaglandin F2α. Domestic Animal Endocrinology 13, 383–398.Google Scholar
Wiltbank, MC, Baez, GM, Cochrane, F, Barletta, RV, Trayford, CR and Joseph, RT 2015. Effect of a second treatment with prostaglandin F2α during the Ovsynch protocol on luteolysis and pregnancy in dairy cows. Journal of Dairy Science 98, 8644–8654.Google Scholar
Xu, ZZ, Garverick, HA, Smith, GW, Smith, MF, Hamilton, SA and Youngquist, RS 1995. Expression of follicle-stimulating hormone and luteinizing hormone receptor messenger ribonucleic acids in bovine follicles during the first follicular wave. Biology of Reproduction 53, 951–957.Google Scholar