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MicroRNA-499-5p regulates porcine myofiber specification by controlling Sox6 expression

Published online by Cambridge University Press:  11 May 2017

X. Y. Wang ,
X. L. Chen ,
Z. Q. Huang ,
D. W. Chen ,
B. Yu ,
J. He ,
J. Q. Luo ,
Y. H. Luo ,
H. Chen  and
P. Zheng
...Show all authors
X. Y. Wang
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
X. L. Chen
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
Z. Q. Huang*
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
D. W. Chen
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
B. Yu
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
J. He
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
J. Q. Luo
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
Y. H. Luo
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
H. Chen
Affiliation:
College of Food Science, Sichuan Agricultural University, Yaan, Sichuan 625014, China
P. Zheng
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
J. Yu
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
*
†E-mail: [email protected]
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Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression of target messenger RNAs (mRNAs) and miRNAs have been proven to play vital roles in skeletal muscle development. The miRNA-499-5p has been reported to be negatively related with the expression of Sox6, a critical transcription factor for the maintenance of fast-twitch skeletal muscle. In this study, we amplified a length of 2012-bp mRNA that contains a 1512-bp porcine Sox6 (pSox6) 3'UTR from skeletal muscle of a Duroc×Landrace×Yorkshire pig. By luciferase reporter assay we verified that pSox6 is a target of miR-499-5p. In extensor digitorum longus and Soleus muscles of pigs, the expression levels of miR-499-5p and pSox6 mRNA were also inversely correlated. Besides, overexpression of miR-499-5p in porcine satellite cells promoted the expression of MyHC I and MyHC IIa mRNA, along with a reduction of pSox6 mRNA. Taken together, these results indicate that miR-499-5p may facilitate the oxidative myofibers formation by downregulating pSox6 expression.

Keywords

skeletal musclemiR-499-5pporcine Sox6porcine satellite cellsoxidative myofibers formation
Type
Research Article
Information
animal , Volume 11 , Issue 12 , December 2017 , pp. 2268 - 2274
Copyright
© The Animal Consortium 2017 

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Footnotes

a

These authors contributed equally to this work.

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