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Metabolomics reveals the mechanism of (−)-hydroxycitric acid promotion of protein synthesis and inhibition of fatty acid synthesis in broiler chickens

Published online by Cambridge University Press:  07 September 2017

M. L. Peng ,
J. Han ,
L. L. Li  and
H. T. Ma
M. L. Peng
Affiliation:
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
J. Han
Affiliation:
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
L. L. Li
Affiliation:
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
H. T. Ma*
Affiliation:
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
*
†E-mail: [email protected]
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Abstract

(−)-Hydroxycitric acid (HCA), a major component of Garcinia cambogia extracts, has been shown to suppress BW gain and fat accumulation in animals and humans. However, the mechanism remains unknown. In this study, gas chromatography-mass spectrometry was used to analyse serum metabolites, and principal component analysis and partial least-squares-discriminant analysis models were generated to analyse serum metabolite changes in broiler chickens after the administration of (−)-HCA at 0, 1000, 2000 and 3000 mg/kg diets for 28 days. Metabolites showing significant changes were screened by ‘variable importance in the projection’ plots. The results showed that 20 metabolites in the 1000 mg/kg (−)-HCA treatment group and 16 metabolites in 3000 mg/kg (−)-HCA treatment group were significantly altered. Metabolites pathway enrichment analysis indicated that these metabolites were mainly associated with metabolism of amino acids, protein synthesis, citric acid cycle, and uric acid and fatty acid synthesis. The data indicated that (−)-HCA promoted protein synthesis by regulating the metabolic directions of amino acids. At the same time, (−)-HCA treatment inhibited fatty acid synthesis by promoting the citric acid cycle, resulting in reduced cytosolic acetyl-CoA content in broiler chickens. The present study identified global changes in metabolites and analysed the main canonical metabolic pathways in broiler chickens supplemented with (−)-HCA. These results will deepen our understanding of the mechanism of (−)-HCA’s effects in animals.

Keywords

(−)-hydroxycitric acidmetabolitesprotein synthesisfatty acid synthesisbroiler chickens
Type
Research Article
Information
animal , Volume 12 , Issue 4 , April 2018 , pp. 774 - 783
Copyright
© The Animal Consortium 2017 

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References

Aggrey, SE, Lee, J, Karnuah, AB and Rekaya, R 2014. Transcriptomic analysis of genes in the nitrogen recycling pathway of meat-type chickens divergently selected for feed efficiency. Animal Genetics 45, 215–222.Google Scholar
Alti̇ner, A, Ates, A, Gursel, FE and Bi̇lal, T 2012. Effect of the antiobesity agent Garcinia cambogia extract on serum lipoprotein (a), apolipoproteins a1 and b, and total cholesterol levels in female rats fed atherogenic diet. Journal of Animal and Plant Sciences 22, 872–877.Google Scholar
Amin, KA, Kamel, HH and Eltawab, MAA 2011. Protective effect of Garcinia against renal oxidative stress and biomarkers induced by high fat and sucrose diet. Lipids in Health and Disease 10, 133–143.Google Scholar
Baker, N and Schotz, MC 1967. Quantitative aspects of free fatty acid metabolism in the fasted rat. Journal of Lipid Research 8, 646–660.Google Scholar
Bei, Y, Jia, H, Zhang, C, Xin, H, Ming, G, Shi, A, Zha, W, Shi, L, Huang, C and Yang, L 2016. Serum metabolomic profiling in patients with systemic lupus erythematosus by GC/MS. Modern Rheumatology 26, 1–25.Google Scholar
Fouad, AM and El-Senousey, HK 2014. Nutritional factors affecting abdominal fat deposition in poultry: a review. Asian Australasian Journal of Animal Sciences 27, 1057–1068.Google Scholar
Han, N, Li, L, Peng, M and Ma, H 2016a. (-)-Hydroxycitric acid nourishes protein synthesis via altering metabolic directions of amino acids in male rats. Phytotherapy Research 30, 1316–1329.Google Scholar
Han, J, Li, LL, Wang, D and Ma, HT 2016b. (-)-Hydroxycitric acid reduced fat deposition via regulating lipid metabolism-related gene expression in broiler chickens. Lipids in Health and Disease 15, 37–50.CrossRefGoogle ScholarPubMed
Hunter, JE, Jun, Z and Kris-Etherton, PM 2009. Cardiovascular disease risk of dietary stearic acid compared with trans, other saturated, and unsaturated fatty acids: a systematic review. American Journal of Clinical Nutrition 91, 46–63.Google Scholar
Jiye, A, Johan, T, Jonas, G, Johansson, AI, Jonsson, P, Henrik, A, Marklund, SL and Thomas, M 2005. Extraction and GC/MS analysis of the human blood plasma metabolome. Analytical Chemistry 77, 8086–8094.Google Scholar
Leonhardt, M, Hrupka, B and Langhans, W 2001. Effect of hydroxycitrate on food intake and body weight regain after a period of restrictive feeding in male rats. Physiology and Behavior 74, 191–196.Google Scholar
Lossow, WJ and Chaikoff, IL 1955. Carbohydrate sparing of fatty acid oxidation. I. The relation of fatty acid chain length to the degree of sparing. II. The mechanism by which carbohydrate spares the oxidation of palmitic acid. Archives of Biochemistry and Biophysics 57, 23–40.Google Scholar
Malley, TJ, Butts, J and Wiedmann, M 2015. Seek and destroy process: listeria monocytogenes process controls in the ready-to-eat meat and poultry industry. Journal of Food Protection 78, 436–445.CrossRefGoogle ScholarPubMed
Márquez, F, Babio, N, Bulló, M and Salassalvadó, J 2012. Evaluation of the safety and efficacy of hydroxycitric acid or Garcinia cambogia extracts in humans. Critical Reviews in Food Science and Nutrition 52, 585–594.Google Scholar
Nicholson, JK, John, C, Lindon, JC and Elaine, H 2002. Metabonomics: a platform for studying drug toxicity and gene function. Nature Reviews Drug Discovery 1, 153–161.CrossRefGoogle ScholarPubMed
Oon, CL, Yong, HW, Boon Kee, B and Swee Keong, Y 2013. Updates on antiobesity effect of garcinia origin (-)-HCA. Evidence-Based Complementary and Alternative Medicine: eCAM 2013, 751658.Google Scholar
Özdoǧan, M and Akşit, M 2003. Effects of feeds containing different fats on carcass and blood parameters of broilers. Journal of Applied Poultry Research 12, 251–256.CrossRefGoogle Scholar
Pei-Pei, H, Jian, Z and Ying-Jin, Y 2009. Analysis of phospholipids, sterols, and fatty acids in Taxus chinensis var. mairei cells in response to shear stress. Biotechnology and Applied Biochemistry 54, 105–112.Google Scholar
Preuss, HG, Rao, CV, Garis, R, Bramble, JD, Ohia, SE, Bagchi, M and Bagchi, D 2004. An overview of the safety and efficacy of a novel, natural(-)-hydroxycitric acid extract (HCA-SX) for weight management. Journal of Medicine 35, 33–48.Google Scholar
Ramalingam, S and Sridhar Gopalakrishna, M 2015. Efficacy of Garcinia cambogia on body weight, inflammation and glucose tolerance in high fat fed male wistar rats. Journal of Clinical and Diagnostic Research Jcdr 9, 1–4.Google Scholar
Smith, GI, Atherton, P, Reeds, DN, Mohammed, BS, Jaffery, H, Rankin, D, Rennie, MJ and Mittendorfer, B 2009. No major sex differences in muscle protein synthesis rates in the postabsorptive state and during hyperinsulinemia-hyperaminoacidemia in middle-aged adults. Journal of Applied Physiology 107, 1308–1315.Google Scholar
Wu, GQ, Deng, XM, Li, JY, Li, N and Yang, N 2006. A potential molecular marker for selection against abdominal fatness in chickens. Poultry Science 85, 1896–1899.Google Scholar
Yang, CM, Chen, AG, Hong, QH, Liu, JX and Liu, JS 2006. Effects of cysteamine on growth performance, digestive enzyme activities, and metabolic hormones in broilers. Poultry Science 85, 1912–1916.Google Scholar
Yi, L, Shi, S, Wang, Y, Huang, W, Xia, ZA, Xing, Z, Peng, W and Wang, Z 2016. Serum metabolic profiling reveals altered metabolic pathways in patients with post-traumatic cognitive impairments. Scientific Reports 6, 21320.CrossRefGoogle ScholarPubMed
Yun, JK, Kim, KY, Min, SK, Jin, HL, Kang, PL and Park, T 2008. A mixture of the aqueous extract of Garcinia cambogia, soy peptide and L: carnitine reduces the accumulation of visceral fat mass in rats rendered obese by a high fat diet. Genes and Nutrition 2, 353–358.Google Scholar
Zhou, CX, Zhou, DH, Elsheikha, HM, Zhao, Y, Suo, X and Zhu, XQ 2016. Metabolomic profiling of mice serum during toxoplasmosis progression using liquid chromatography-mass spectrometry. Scientific Reports 6, 19557.CrossRefGoogle ScholarPubMed
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